##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779819_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	47106
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.46363520570628	23.0	12.0	32.0	12.0	34.0
2	21.832696471787035	23.0	12.0	32.0	12.0	34.0
3	21.444953933681486	23.0	11.0	32.0	11.0	34.0
4	21.77402029465461	23.0	12.0	32.0	12.0	34.0
5	21.017407548932194	21.0	11.0	32.0	11.0	34.0
6	23.816074385428607	26.0	12.0	34.0	11.0	38.0
7	23.803549441684712	25.0	12.0	34.0	11.0	38.0
8	23.31131915254957	24.0	11.0	34.0	11.0	38.0
9	24.18551776843714	26.0	11.0	36.0	11.0	38.0
10-11	23.223124442746148	24.0	11.5	34.0	11.0	38.0
12-13	23.267004203286206	24.0	11.0	34.0	11.0	38.0
14-15	22.62406062921921	23.0	11.0	34.0	11.0	37.5
16-17	22.67559334267397	22.5	11.0	33.5	11.0	38.0
18-19	22.53095147115017	22.0	11.0	34.0	10.5	38.0
20-21	22.619761813781682	22.5	11.0	33.5	10.5	38.0
22-23	22.80004670318006	22.5	11.0	34.0	10.5	38.0
24-25	23.018575128433746	23.0	11.0	34.0	10.0	38.0
26-27	22.231700844902985	22.0	11.0	34.0	10.0	38.0
28-29	22.304324289899377	22.0	11.0	34.0	10.5	38.0
30-31	22.42642126268416	21.0	11.0	34.0	11.0	38.0
32-33	22.62226680253046	21.5	11.0	34.0	11.0	38.0
34-35	22.70426272661657	22.5	11.0	34.0	11.0	38.0
36-37	22.510338385768264	21.5	11.0	34.0	11.0	38.0
38-39	22.34619793656859	21.0	11.0	34.0	11.0	38.0
40-41	22.270708614613852	21.0	11.0	34.0	11.0	38.0
42-43	22.49038339065087	21.5	11.0	34.0	11.0	38.0
44-45	22.67718549653972	22.5	11.0	34.0	11.0	38.0
46-47	22.516558400203795	22.0	11.0	34.0	11.0	38.0
48-49	22.137413492973295	21.0	11.0	34.0	11.0	38.0
50-51	21.897613892073196	21.0	11.0	33.5	11.0	38.0
52-53	22.32642338555598	21.5	11.0	34.0	11.0	38.0
54-55	22.284040249649728	21.5	11.0	34.0	11.0	38.0
56-57	22.208826901031713	21.5	11.0	34.0	11.0	38.0
58-59	22.149418333121048	21.0	11.0	34.0	11.0	38.0
60-61	22.37009085891394	22.0	11.0	34.0	11.0	38.0
62-63	22.070288285993293	21.0	11.0	34.0	11.0	38.0
64-65	22.034411752218404	21.0	11.0	34.0	11.0	38.0
66-67	22.457107374856704	22.5	11.0	34.0	11.0	38.0
68-69	22.22070436887021	22.0	11.0	33.0	11.0	38.0
70-71	22.382297371884686	22.5	11.0	34.0	11.0	38.0
72-73	22.14962000594404	21.5	11.0	33.0	11.0	38.0
74-75	22.263480236063344	22.0	11.0	33.0	11.0	38.0
76	21.941111535685476	22.0	11.0	33.0	11.0	37.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
11	3.0
12	59.0
13	565.0
14	1866.0
15	3290.0
16	4314.0
17	5146.0
18	5236.0
19	4632.0
20	3511.0
21	2564.0
22	1704.0
23	1208.0
24	836.0
25	693.0
26	523.0
27	467.0
28	480.0
29	483.0
30	458.0
31	582.0
32	646.0
33	712.0
34	961.0
35	1295.0
36	1936.0
37	2936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.133188978049507	20.891181590455567	9.090137137519637	48.88549229397529
2	10.977370186388145	21.262684159130472	28.36581327219462	39.394132382286756
3	18.56875981828217	20.03990999023479	33.62416677281026	27.76716341867278
4	19.07824905532204	33.859805544941196	18.169659915934275	28.89228548380249
5	10.368105973761304	26.981700844902985	26.334224939498153	36.31596824183756
6	15.628582346197936	48.0851696174585	10.376597461045302	25.909650575298265
7	14.986942952378932	37.79112970000637	11.460478546103054	35.76144880151165
8	13.4632530887785	43.65473612703265	16.81314482231563	26.06886596187322
9	18.603367375103506	22.155459776216055	12.418522686256608	46.82265016242383
10-11	14.230213994565217	34.78367017663043	20.963187839673914	30.022927989130434
12-13	14.118308902357473	24.33792232329558	24.712613176805256	36.83115559754169
14-15	26.925444741646498	28.22570373200866	16.765380206343142	28.083471320001696
16-17	28.9520332020677	24.3708271857851	15.382492490261221	31.294647121885976
18-19	19.085022821356542	23.964547287973677	21.307716802887168	35.64271308778261
20-21	26.409586889143633	23.238016388570458	17.404364624463973	32.94803209782194
22-23	28.680607154229914	22.41481795987687	15.966457913172698	32.938116972720515
24-25	18.097189198369566	32.918648097826086	14.734417459239129	34.24974524456522
26-27	24.58285568717361	32.88859168683395	16.562645947437694	25.965906678554752
28-29	19.482421875	30.0239894701087	21.493928328804348	28.999660326086957
30-31	21.473304320135867	27.71680288716697	22.083642925379472	28.726249867317694
32-33	16.645437948456674	30.784825712223494	22.368700377871185	30.201035961448646
34-35	15.671705163043478	35.028872282608695	20.368758491847828	28.9306640625
36-37	23.9192288092407	25.36945812807882	21.20349923560387	29.50781382707661
38-39	25.01857710027388	22.344536209422305	24.8572217150379	27.779664975265916
40-41	19.242975873305664	29.986944199721897	21.517657173790745	29.25242275318169
42-43	13.667338923681138	29.20709054240527	24.10465980256873	33.02091073134486
44-45	13.405727872959257	24.10143727575738	29.068212215782435	33.424622635500924
46-47	17.337536887247097	27.553446701908584	24.245801753603804	30.86321465724052
48-49	18.95830679743557	28.602513480236063	22.331550121003694	30.10762960132467
50-51	26.96239212814062	27.459159952870742	16.903905147066627	28.674542771922003
52-53	12.258763083586336	32.586356977558864	21.509097471391268	33.64578246746353
54-55	16.161015689688117	26.265896690091505	21.03246215579287	36.54062546442751
56-57	18.545176597980827	29.142117052560064	18.672569190101594	33.64013715935752
58-59	16.70187805887911	30.251717218901618	19.452613251515505	33.59379147070376
60-61	13.205143178705287	27.23633776796237	21.21720480341463	38.341314249917716
62-63	16.477357168637607	28.51425659752447	19.869004904354473	35.13938132948345
64-65	17.39375086262727	26.59015383962034	19.614817016849102	36.401278280903284
66-67	13.716039027911965	36.867362430857106	15.15038911125503	34.266209429975895
68-69	14.986572693209924	39.620638778911164	13.745740943201962	31.647047584676947
70-71	12.799855644716171	37.19589861164183	15.606292192077442	34.397953551564555
72-73	12.518045176630435	37.00534986413043	18.086574388586957	32.39003057065217
74-75	12.742308753901357	33.650395974436826	17.217987643049746	36.38930762861207
76	10.557265683048508	31.62509287761384	15.327459929943743	42.490181509393906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.5
14	1.0
15	1.0
16	4.0
17	7.0
18	6.0
19	4.0
20	6.5
21	10.0
22	12.5
23	20.0
24	28.5
25	32.0
26	34.0
27	45.5
28	90.0
29	125.0
30	132.0
31	178.5
32	252.0
33	286.0
34	342.0
35	442.0
36	595.5
37	705.0
38	822.0
39	1085.5
40	1359.5
41	1650.5
42	1814.0
43	1983.0
44	2319.0
45	2616.5
46	2747.0
47	2858.0
48	2978.0
49	2976.0
50	2965.0
51	2951.0
52	2881.5
53	2691.0
54	2556.0
55	2431.5
56	2234.5
57	1967.5
58	1773.0
59	1659.5
60	1400.0
61	1155.5
62	1057.0
63	970.0
64	802.5
65	664.0
66	516.0
67	426.0
68	403.0
69	314.5
70	242.5
71	236.0
72	213.5
73	165.0
74	125.5
75	112.0
76	101.0
77	82.5
78	65.0
79	55.0
80	47.5
81	38.5
82	32.5
83	28.0
84	25.0
85	17.0
86	12.5
87	13.0
88	10.0
89	7.0
90	7.5
91	5.0
92	2.0
93	5.5
94	5.5
95	2.0
96	2.0
97	1.5
98	2.5
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.01061435910499724
8	0.0
9	0.014860102746996135
10-11	0.0042457436419988965
12-13	0.0010614359104997241
14-15	0.0
16-17	0.0010614359104997241
18-19	0.0021228718209994482
20-21	0.0
22-23	0.0021228718209994482
24-25	0.0042457436419988965
26-27	0.0
28-29	0.0042457436419988965
30-31	0.0021228718209994482
32-33	0.0
34-35	0.0042457436419988965
36-37	0.02122871820999448
38-39	0.01061435910499724
40-41	0.0010614359104997241
42-43	0.0021228718209994482
44-45	0.006368615462998345
46-47	0.006368615462998345
48-49	0.0
50-51	0.0031843077314991724
52-53	0.01061435910499724
54-55	0.01061435910499724
56-57	0.015921538657495862
58-59	0.020167282299494757
60-61	0.030781641404491997
62-63	0.01061435910499724
64-65	0.024413025941493652
66-67	0.024413025941493652
68-69	0.0010614359104997241
70-71	0.0
72-73	0.0042457436419988965
74-75	0.014860102746996135
76	0.0021228718209994482
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	47106.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.55271090731542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.08352043107318	91.79510041183714
2	1.493039964077234	2.823419521929266
3	0.7431522227211496	2.108011718252452
4	0.35473731477323756	1.341654990871651
5	0.1571621014818141	0.7430051373498069
6	0.07633587786259542	0.43306585148388743
7	0.042658284687920975	0.2823419521929266
8	0.024696901661427927	0.18681272024795142
9	0.011225864391558149	0.09552923194497516
>10	0.01347103726986978	0.19105846388995032
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014860102746996137	0.0
2	0.0	0.0	0.0	0.014860102746996137	0.0
3	0.0	0.0	0.0	0.014860102746996137	0.0
4	0.0	0.0	0.0	0.014860102746996137	0.0
5	0.0	0.0	0.0	0.019105846388995033	0.0
6	0.0	0.0	0.0	0.019105846388995033	0.0
7	0.0	0.0	0.0	0.019105846388995033	0.0
8	0.0	0.0	0.0	0.019105846388995033	0.0
9	0.0	0.0	0.0	0.019105846388995033	0.0
10	0.0	0.0	0.0	0.02122871820999448	0.0
11	0.0	0.0	0.0	0.02122871820999448	0.0
12	0.0	0.0	0.0	0.025474461851993375	0.0
13	0.0	0.0	0.0	0.029720205493992274	0.0
14	0.0	0.0	0.0	0.03184307731499172	0.0
15	0.0	0.0	0.0	0.03184307731499172	0.0
16	0.0	0.0	0.0	0.03184307731499172	0.0
17	0.0	0.0	0.0	0.03821169277799007	0.0
18	0.0	0.0	0.0	0.040334564598989514	0.0
19	0.0	0.0	0.0	0.04245743641998896	0.0
20	0.0	0.0	0.0	0.0530717955249862	0.0
21	0.0	0.0	0.0	0.0530717955249862	0.0
22	0.0	0.0	0.0	0.06793189827198234	0.0
23	0.0	0.0	0.0	0.06793189827198234	0.0
24	0.0	0.0	0.0	0.07217764191398124	0.0
25	0.0	0.0	0.0	0.08066912919797903	0.0
26	0.0	0.0	0.0	0.08916061648197682	0.0
27	0.0	0.0	0.0	0.09765210376597461	0.0
28	0.0	0.0	0.0	0.11251220651297075	0.0
29	0.0	0.0	0.0	0.14223241200696302	0.0
30	0.0	0.0	0.0	0.1592153865749586	0.0
31	0.0	0.0	0.0	0.17407548932195474	0.0
32	0.0	0.0	0.0	0.20167282299494757	0.0
33	0.0	0.0	0.0	0.25262174669893434	0.0
34	0.0	0.0	0.0	0.2950791831189233	0.0
35	0.0	0.0	0.0	0.3396594913599117	0.0
36	0.0	0.0	0.0	0.4776461597248758	0.0
37	0.0	0.0	0.0	0.6177556999108393	0.0
38	0.0	0.0	0.0	0.7790939583067974	0.0
39	0.0	0.0	0.0	0.9552923194497516	0.0
40	0.0	0.0	0.0	1.1909310915806903	0.0
41	0.0	0.0	0.0	1.4223241200696302	0.0
42	0.0	0.0	0.0	1.5645565320765933	0.0
43	0.0	0.0	0.0	1.7534921241455441	0.0
44	0.0	0.0	0.0	2.018851101770475	0.0
45	0.0	0.0	0.0	2.2056638220184266	0.0
46	0.0	0.0	0.0	2.4137052604763722	0.0
47	0.0	0.0	0.0	2.628115314397317	0.0
48	0.0	0.0	0.0	2.8021908037192715	0.0
49	0.0	0.0	0.0	3.0059864985352185	0.0
50	0.0	0.0	0.0	3.211905065172165	0.0
51	0.0	0.0	0.0	3.4220693754511102	0.0
52	0.0	0.0	0.0	3.6470937884770516	0.0
53	0.0	0.0	0.0	3.823292149620006	0.0
54	0.0	0.0	0.0	4.005859126225959	0.0
55	0.0	0.0	0.0	4.175688871905915	0.0
56	0.0	0.0	0.0	4.366747335795865	0.0
57	0.0	0.0	0.0	4.572665902432811	0.0
58	0.0	0.0	0.0	4.763724366322761	0.0
59	0.0	0.0	0.0	4.910202521971724	0.0
60	0.0	0.0	0.0	5.043943446694689	0.0
61	0.0	0.0	0.0	5.209527448732645	0.0
62	0.0	0.0	0.0	5.3687428353076045	0.0
63	0.0	0.0	0.0	5.566169914660553	0.0
64	0.0	0.0	0.0	5.725385301235511	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGAT	15	0.0022116443	70.00001	37
GCTGCAA	15	0.0022116443	70.00001	60
AGTTGTA	15	0.0022116443	70.00001	34
CTCGGGG	15	0.0022116443	70.00001	44
TCGTCAT	15	0.0022116443	70.00001	8
GAAGTCG	15	0.0022116443	70.00001	14
TGTAGTC	15	0.0022116443	70.00001	18
ATCGGTG	15	0.0022116443	70.00001	44
ATGCATA	15	0.0022116443	70.00001	28
CTGATGC	15	0.0022116443	70.00001	12
CTTACTG	15	0.0022116443	70.00001	42
CGGCCAT	20	7.8500656E-5	70.0	2
CCGGATC	20	7.8500656E-5	70.0	55
CTTAGGT	20	7.8500656E-5	70.0	31
ATCTCGG	80	0.0	65.625	42
GATCTCG	75	0.0	65.333336	41
TCTCGGT	75	0.0	60.666668	43
GCCCCTA	30	8.256509E-6	58.333336	68
ATATGTA	30	8.256509E-6	58.333336	26
ATGCAGT	30	8.256509E-6	58.333336	29
>>END_MODULE