SRR1779819_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1779819_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	47106
Sequences flagged as poor quality	0
Sequence length	76
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	23595	50.089160616481976	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	3257	6.914193520995203	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	1529	3.245871014308156	No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	557	1.1824396042966927	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT	539	1.1442279115187024	No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT	520	1.103893346919713	RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT	475	1.0083641149747378	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT	256	0.5434551861758588	No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	248	0.5264722116078632	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT	235	0.4988748779348703	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG	148	0.3141850295079183	No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	137	0.2908334394769244	No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	130	0.27597333672992824	No Hit
CCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	100	0.21228718209994482	No Hit
TTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC	96	0.20379569481594703	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCG	91	0.19318133571094978	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC	90	0.19105846388995032	No Hit
TATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCT	74	0.15709251475395916	RNA PCR Primer, Index 30 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG	73	0.1549696429329597	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCG	67	0.14223241200696302	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCAGT	67	0.14223241200696302	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCC	59	0.12524943743896744	No Hit
CTTATACACATCTCCGAGCCCACGAGAAATTGCCGGATCTCGTATGCCGT	58	0.12312656561796799	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTCTGCCGT	56	0.11888082197596908	No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGACGGATCTCGTATGCCGT	54	0.11463507833397019	No Hit
CTTATACACCTCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT	48	0.10189784740797352	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTACA	20	7.80938E-5	70.073326	1
CTCTATA	15	0.002202466	70.073326	1
AACGAAA	15	0.0022117777	69.99894	70
ACCATCA	15	0.0022117777	69.99894	43
ACCACAA	15	0.0022117777	69.99894	70
TGATTGA	20	7.850656E-5	69.99894	55
TTCTGAT	20	7.850656E-5	69.99894	52
CTGATTG	20	7.850656E-5	69.99894	54
CTTAGCT	15	0.0022117777	69.99894	14
TCTATAC	15	0.0022117777	69.99894	2
GACAAAA	25	2.7991427E-6	69.99894	60
TCTGATT	20	7.850656E-5	69.99894	53
GCTTGAC	25	2.7991427E-6	69.99894	56
CTTGACA	25	2.7991427E-6	69.99894	57
CGATCTC	20	7.850656E-5	69.99894	35
TCTTTAT	85	0.0	65.95136	1
TTGCAAA	40	1.03809725E-8	61.24907	58
TGCAAAA	40	1.03809725E-8	61.24907	59
GCAAAAA	40	1.03809725E-8	61.24907	60
GTCTGCC	30	8.257253E-6	58.33245	42

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content