FastQCFastQC Report
Wed 25 May 2016
SRR1779818_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779818_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219164
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT501.4551915E-1163.01766654
TGGTCGC1200.061.2392249
CGGTGGT1450.053.094146
CGTATTT200.00693060952.5267156
TCGCCGT800.052.5027452
TGATACA200.006949405752.4907572
TGCGCGA200.006949405752.49075714
GACGGCA200.006949405752.49075767
CAATACG200.006949405752.49075711
GTAGATC1550.049.6800138
AGCGATC305.8674254E-446.66909827
TCGGTGG1500.046.6584545
GTCGCCG1450.045.85399651
GTGGTCG1700.045.28614848
ATCTCGG1350.044.06631542
AGATCTC1750.043.9922640
TAGATCT1750.043.9922639
TCTCGGT1150.042.60119243
AGGTGTA1250.042.0021934
GATCTCG1500.041.99260741
Produced by FastQC (version 0.11.5)