##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779818_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219164
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.244656969210272	34.0	23.0	34.0	12.0	34.0
2	28.96982168604333	34.0	24.0	34.0	12.0	34.0
3	28.617126900403353	34.0	23.0	34.0	11.0	34.0
4	28.958975926703292	34.0	24.0	34.0	12.0	34.0
5	29.574318774981293	34.0	31.0	34.0	11.0	34.0
6	31.970547170155683	37.0	28.0	38.0	12.0	38.0
7	31.8244191564308	37.0	27.0	38.0	12.0	38.0
8	31.250529284006497	37.0	27.0	38.0	11.0	38.0
9	32.29732529064992	37.0	31.0	38.0	12.0	38.0
10-11	30.840776769907468	36.5	25.5	38.0	11.0	38.0
12-13	31.6329803252359	37.0	27.5	38.0	11.0	38.0
14-15	30.45534622474494	36.5	24.5	38.0	11.0	38.0
16-17	31.08158958588089	36.5	26.0	38.0	11.0	38.0
18-19	32.01020696829771	37.0	31.0	38.0	11.0	38.0
20-21	30.644905185158148	36.5	25.5	38.0	11.0	38.0
22-23	31.55142039751054	37.0	28.5	38.0	11.0	38.0
24-25	31.90690533116753	37.0	29.0	38.0	11.0	38.0
26-27	30.76574163640014	37.0	25.0	38.0	11.0	38.0
28-29	31.978486430253145	37.0	29.0	38.0	11.0	38.0
30-31	32.43563039550291	37.5	31.0	38.0	11.0	38.0
32-33	32.57030123560439	38.0	31.5	38.0	11.0	38.0
34-35	32.459350075742364	37.5	31.5	38.0	11.0	38.0
36-37	31.904391232136664	37.0	29.0	38.0	11.0	38.0
38-39	31.817876111040135	37.0	29.0	38.0	11.0	38.0
40-41	31.812211403332668	37.0	29.0	38.0	11.0	38.0
42-43	32.25271029913672	37.0	30.0	38.0	11.0	38.0
44-45	32.32765873957402	37.0	31.0	38.0	11.0	38.0
46-47	32.22417915351062	37.0	31.0	38.0	11.0	38.0
48-49	31.865046266722636	37.0	29.0	38.0	11.0	38.0
50-51	31.207915077293716	37.0	26.0	38.0	11.0	38.0
52-53	31.82314613713931	37.0	29.0	38.0	11.0	38.0
54-55	31.982303206731032	37.0	29.0	38.0	11.0	38.0
56-57	32.096245277509084	37.0	30.5	38.0	11.0	38.0
58-59	32.361852311511015	37.0	31.0	38.0	11.0	38.0
60-61	32.20680859995255	37.0	31.0	38.0	11.0	38.0
62-63	32.22480425617346	37.0	31.0	38.0	11.0	38.0
64-65	32.23213666478071	37.0	31.0	38.0	11.0	38.0
66-67	32.092750177948936	37.0	31.0	38.0	11.0	38.0
68-69	31.39894781989743	37.0	27.0	38.0	11.0	38.0
70-71	31.369524648208646	37.0	26.5	38.0	11.0	38.0
72-73	31.540161705389572	37.0	27.0	38.0	11.0	38.0
74-75	31.588509061707214	37.0	28.0	38.0	11.0	38.0
76	30.43472924385392	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	50.0
13	639.0
14	2005.0
15	3544.0
16	5013.0
17	5955.0
18	6262.0
19	5553.0
20	4715.0
21	3607.0
22	2987.0
23	2588.0
24	2608.0
25	2729.0
26	2992.0
27	3480.0
28	4089.0
29	4766.0
30	5744.0
31	6990.0
32	8729.0
33	10774.0
34	14454.0
35	20605.0
36	33079.0
37	55206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.625248672227194	22.49502655545619	9.516617692686754	30.363107079629863
2	19.29924622657006	24.777791973134274	30.505922505520978	25.41703929477469
3	17.340895402529615	22.072968188206094	32.82519026847475	27.760946140789542
4	12.976127466189702	22.254111076636672	30.713073314960486	34.05668814221314
5	13.63453851909985	33.272343998101874	34.8232373930025	18.26988008979577
6	26.348906501672303	40.636250393549886	16.36209327474573	16.652749830032075
7	25.980953597780506	28.65767126475594	18.80638287192732	26.55499226553623
8	23.002546154758754	36.38582914297708	18.821468008797467	21.790156693466695
9	22.876282261891724	18.522524002482385	19.10113897711094	39.50005475851495
10-11	22.831088773245057	27.083014881855455	23.941923576208197	26.1439727686913
12-13	23.016325673924552	23.47625522439817	25.2258582613933	28.281560840283987
14-15	24.46267039297325	25.22169622996635	21.792277419722808	28.523355957337593
16-17	25.10340453588303	26.09262034964764	22.861014721885716	25.94296039258362
18-19	21.878685973849976	25.88514705110685	25.218734386300323	27.01743258874285
20-21	24.329725684875253	25.301372488182366	24.46318738478947	25.90571444215291
22-23	25.220390954388655	25.453330048002336	23.48966033327858	25.83661866433043
24-25	21.672541437094328	28.249090266132804	23.47923297172125	26.59913532505162
26-27	23.605718118478027	27.920315016677083	23.988538211285665	24.485428653559225
28-29	22.132177968209206	27.49125604889768	25.468454470993812	24.908111511899303
30-31	22.38247875434894	27.04271944333542	25.067472765641934	25.507329036673703
32-33	21.539626808296088	27.745495942526926	24.860663386010902	25.85421386316608
34-35	21.723338626366605	27.969802332585008	25.22563835806457	25.081220682983812
36-37	23.926069594980035	25.766343411294923	25.05693097547062	25.250656018254418
38-39	23.281335916092278	25.353703361465328	26.237591006888117	25.127369715554277
40-41	22.08794844008441	26.580505332801003	25.665430901728172	25.666115325386414
42-43	20.443413250837843	26.318707266492826	26.519470623529923	26.71840885913941
44-45	19.82163268379157	24.801681005865724	27.954251452742245	27.422434857600457
46-47	20.927643361509414	24.76711953731905	27.289962698059618	27.015274403111917
48-49	20.514136706157732	25.594134059731662	27.75895621305555	26.132773021055055
50-51	22.891214429607526	25.45075412768268	25.91411317276608	25.743918269943716
52-53	18.422505646946085	26.654726322754343	27.21211982933674	27.710648200962833
54-55	18.669973442362625	26.093335036916027	26.877743604720138	28.358947916001203
56-57	19.208128174355092	26.659106776977172	26.262326651120976	27.870438397546764
58-59	19.194631791258637	26.797920982741786	26.304861687855364	27.702585538144213
60-61	18.725740534101877	25.79415878840155	26.172726256243855	29.307374421252717
62-63	19.33982390132268	25.701782206230305	26.28931018511124	28.669083707335773
64-65	20.00739324294105	25.187683404907833	25.561681095660347	29.243242256490763
66-67	19.52986489662458	27.34057870153814	24.012622730498315	29.116933671338963
68-69	19.68512020733427	28.294465762925313	23.092629686580306	28.927784343160113
70-71	19.836560376157525	27.619562564021837	23.429540046586226	29.114337013234408
72-73	19.236104456703764	27.501790986205982	23.87875137461043	29.383353182479823
74-75	18.879810533376837	26.887878379950852	24.09196797502983	30.140343111642476
76	18.37258963852563	26.112211281358654	23.83485886894387	31.68034021117185
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.0
11	1.0
12	0.0
13	2.0
14	11.5
15	14.0
16	15.0
17	21.0
18	27.0
19	41.5
20	54.5
21	59.0
22	102.0
23	197.0
24	307.0
25	365.0
26	470.0
27	652.5
28	908.0
29	1086.0
30	1341.0
31	1813.0
32	2391.5
33	2753.0
34	3235.0
35	4193.5
36	5186.0
37	5702.0
38	6330.5
39	7811.5
40	9299.0
41	10729.5
42	11525.0
43	12010.0
44	13075.0
45	13661.5
46	13668.0
47	13772.0
48	13843.5
49	13402.5
50	12994.0
51	12303.0
52	10620.0
53	9113.0
54	8598.0
55	7866.5
56	6645.5
57	5591.0
58	5026.0
59	4590.5
60	3793.0
61	3183.0
62	2935.0
63	2702.5
64	2325.0
65	2076.0
66	1848.0
67	1724.0
68	1610.0
69	1450.5
70	1313.5
71	1222.0
72	1132.5
73	981.5
74	870.0
75	820.0
76	765.5
77	662.0
78	578.0
79	543.0
80	495.0
81	415.0
82	334.0
83	285.0
84	261.5
85	199.5
86	158.0
87	155.0
88	123.5
89	82.0
90	57.5
91	32.0
92	21.0
93	22.0
94	19.5
95	12.5
96	9.0
97	9.5
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0022813965797302475
7	0.006844189739190742
8	0.004562793159460495
9	0.00912558631892099
10-11	0.002053256921757223
12-13	0.0
14-15	6.844189739190743E-4
16-17	2.2813965797302478E-4
18-19	0.0025095362377032726
20-21	0.0
22-23	0.0036502345275683966
24-25	0.002965815553649322
26-27	4.5627931594604957E-4
28-29	0.005703491449325619
30-31	6.844189739190743E-4
32-33	2.2813965797302478E-4
34-35	0.0036502345275683966
36-37	0.017794893321895933
38-39	0.008441167345001917
40-41	6.844189739190743E-4
42-43	2.2813965797302478E-4
44-45	0.00433465350148747
46-47	0.002965815553649322
48-49	2.2813965797302478E-4
50-51	0.0015969776058111734
52-53	0.00866930700297494
54-55	0.008213027687028892
56-57	0.011406982898651238
58-59	0.01003814495081309
60-61	0.022129546823383403
62-63	0.011635122556624264
64-65	0.02053256921757223
66-67	0.01619791571608476
68-69	4.5627931594604957E-4
70-71	2.2813965797302478E-4
72-73	0.005019072475406545
74-75	0.010266284608786116
76	0.002737675895676297
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	219164.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.29103001216279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.9763536517812	54.34296920217899
2	7.2465632003575084	9.607642771998682
3	4.263054657574372	8.478068527462595
4	2.5497909017033646	6.761130607582297
5	1.4252954700875613	4.724215239188711
6	0.7644359349690102	3.0405147304443827
7	0.4584013459597079	2.1271528167841574
8	0.3036009024397856	1.6100813228284432
9	0.2101009534942309	1.2535027752303074
>10	0.8017003570824284	8.023980720876272
>50	7.026245508803643E-4	0.03074128542519714
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.562793159460495E-4	0.0
2	0.0	0.0	0.0	4.562793159460495E-4	0.0
3	0.0	0.0	0.0	4.562793159460495E-4	0.0
4	0.0	0.0	0.0	9.12558631892099E-4	0.0
5	0.0	0.0	0.0	0.002737675895676297	0.0
6	0.0	0.0	0.0	0.002737675895676297	0.0
7	0.0	0.0	0.0	0.002737675895676297	0.0
8	0.0	0.0	0.0	0.002737675895676297	0.0
9	0.0	0.0	0.0	0.002737675895676297	0.0
10	0.0	0.0	0.0	0.002737675895676297	0.0
11	0.0	0.0	0.0	0.002737675895676297	0.0
12	0.0	0.0	0.0	0.003650234527568396	0.0
13	0.0	0.0	0.0	0.003650234527568396	0.0
14	0.0	0.0	0.0	0.005931631107298644	0.0
15	0.0	0.0	0.0	0.01003814495081309	0.0
16	0.0	0.0	0.0	0.01003814495081309	0.0
17	4.562793159460495E-4	0.0	0.0	0.01049442426675914	0.0
18	4.562793159460495E-4	0.0	0.0	0.01049442426675914	0.0
19	4.562793159460495E-4	0.0	0.0	0.015513496742165685	0.0
20	4.562793159460495E-4	0.0	0.0	0.01825117263784198	0.0
21	4.562793159460495E-4	0.0	0.0	0.019163731269734082	0.0
22	4.562793159460495E-4	0.0	0.0	0.024639083061086677	0.0
23	4.562793159460495E-4	0.0	0.0	0.02965815553649322	0.0
24	4.562793159460495E-4	0.0	0.0	0.03467722801189976	0.0
25	4.562793159460495E-4	0.0	0.0	0.035589786643791865	0.0
26	4.562793159460495E-4	0.0	0.0	0.040608859119198405	0.0
27	4.562793159460495E-4	0.0	0.0	0.07984888029055867	0.0
28	4.562793159460495E-4	0.0	0.0	0.11863262214597288	0.0
29	4.562793159460495E-4	0.0	0.0	0.1701921848478765	0.0
30	4.562793159460495E-4	0.0	0.0	0.21992663028599588	0.0
31	4.562793159460495E-4	0.0	0.0	0.26646712051249294	0.0
32	4.562793159460495E-4	0.0	0.0	0.322133197057911	0.0
33	4.562793159460495E-4	0.0	0.0	0.4042634739281999	0.0
34	4.562793159460495E-4	0.0	0.0	0.5133142304393057	0.0
35	4.562793159460495E-4	0.0	0.0	0.6392473216404154	0.0
36	4.562793159460495E-4	0.0	0.0	0.8815316384077677	0.0
37	4.562793159460495E-4	0.0	0.0	1.2935518607070504	0.0
38	4.562793159460495E-4	0.0	0.0	1.878958223065832	0.0
39	4.562793159460495E-4	0.0	0.0	2.624974904637623	0.0
40	4.562793159460495E-4	0.0	0.0	3.4394334836013214	0.0
41	4.562793159460495E-4	0.0	0.0	4.377543757186399	0.0
42	4.562793159460495E-4	0.0	0.0	5.4041722180650105	0.0
43	4.562793159460495E-4	0.0	0.0	6.385629026664963	0.0
44	4.562793159460495E-4	0.0	0.0	7.339709076308153	0.0
45	4.562793159460495E-4	0.0	0.0	8.320253326276212	0.0
46	4.562793159460495E-4	0.0	0.0	9.337756200835903	0.0
47	4.562793159460495E-4	0.0	0.0	10.337920461389645	0.0
48	4.562793159460495E-4	0.0	0.0	11.29930098008797	0.0
49	4.562793159460495E-4	0.0	0.0	12.197714953185741	0.0
50	4.562793159460495E-4	0.0	0.0	13.00989213556971	0.0
51	4.562793159460495E-4	0.0	0.0	13.890967494661531	0.0
52	4.562793159460495E-4	0.0	0.0	14.800332171342008	0.0
53	4.562793159460495E-4	0.0	0.0	15.632585643627603	0.0
54	4.562793159460495E-4	0.0	0.0	16.512292164771587	0.0
55	4.562793159460495E-4	0.0	0.0	17.33907028526583	0.0
56	4.562793159460495E-4	0.0	0.0	18.055428811301127	0.0
57	4.562793159460495E-4	0.0	0.0	18.835210162252924	0.0
58	4.562793159460495E-4	0.0	0.0	19.583964519720393	0.0
59	4.562793159460495E-4	0.0	0.0	20.28070303517001	0.0
60	4.562793159460495E-4	0.0	0.0	20.945501998503403	0.0
61	4.562793159460495E-4	0.0	0.0	21.615320034312205	0.0
62	4.562793159460495E-4	0.0	0.0	22.30749575660236	0.0
63	4.562793159460495E-4	0.0	0.0	22.97548867514738	0.0
64	4.562793159460495E-4	0.0	0.0	23.570476903141028	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTAT	50	1.4551915E-11	63.017666	54
TGGTCGC	120	0.0	61.23922	49
CGGTGGT	145	0.0	53.0941	46
CGTATTT	20	0.006930609	52.52671	56
TCGCCGT	80	0.0	52.50274	52
TGATACA	20	0.0069494057	52.490757	2
TGCGCGA	20	0.0069494057	52.490757	14
GACGGCA	20	0.0069494057	52.490757	67
CAATACG	20	0.0069494057	52.490757	11
GTAGATC	155	0.0	49.68001	38
AGCGATC	30	5.8674254E-4	46.669098	27
TCGGTGG	150	0.0	46.65845	45
GTCGCCG	145	0.0	45.853996	51
GTGGTCG	170	0.0	45.286148	48
ATCTCGG	135	0.0	44.066315	42
AGATCTC	175	0.0	43.99226	40
TAGATCT	175	0.0	43.99226	39
TCTCGGT	115	0.0	42.601192	43
AGGTGTA	125	0.0	42.00219	34
GATCTCG	150	0.0	41.992607	41
>>END_MODULE