FastQCFastQC Report
Wed 25 May 2016
SRR1779818_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779818_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219164
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2784212.703728714569912No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG36501.665419503203081No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC20580.9390228322169699No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT6660.303882024420069Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC5980.2728550309357376No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT3670.16745450895220018No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT3150.1437279845230056No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC2720.12410797393732546Illumina PCR Primer Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC207.914449E-569.9997745
TCGGATT150.002226550869.96783432
CCGAGTT150.002226550869.96783444
TAATACG207.9323676E-569.967834
GCTTGAC501.4551915E-1163.10061656
TCGTTGT252.3921816E-455.97426234
TATATCG252.3921816E-455.97426236
GCGTCTA252.3921816E-455.97426244
TCGTATG42100.052.68361340
CGTATGC42000.052.64246441
CTCGTAT41900.052.6011139
CTTGACA608.367351E-1152.5478157
TATGCCG41950.052.53841843
CCTTTGG200.006925862352.53580564
GTCTTCT41750.052.5148349
CAACGCG200.00695720452.4758729
AACGCGA200.00695720452.47587210
AACTCGT800.052.47587237
GCCGTCT41650.052.47273346
CCGTCTT41650.052.3886447