##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779818_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219164
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.493977113029516	34.0	34.0	34.0	34.0	34.0
2	33.59422624153602	34.0	34.0	34.0	34.0	34.0
3	33.72587651256593	34.0	34.0	34.0	34.0	34.0
4	33.50360916938914	34.0	34.0	34.0	34.0	34.0
5	33.565749849427824	34.0	34.0	34.0	34.0	34.0
6	37.21407712945557	38.0	38.0	38.0	36.0	38.0
7	37.27749995437207	38.0	38.0	38.0	36.0	38.0
8	37.16656020149295	38.0	38.0	38.0	36.0	38.0
9	37.08076143892245	38.0	38.0	38.0	36.0	38.0
10-11	37.0116830318848	38.0	37.5	38.0	35.0	38.0
12-13	37.20713255826687	38.0	38.0	38.0	36.0	38.0
14-15	37.228078060265375	38.0	38.0	38.0	36.0	38.0
16-17	37.13381987917724	38.0	38.0	38.0	36.0	38.0
18-19	36.98426748918618	38.0	37.5	38.0	35.5	38.0
20-21	36.994463050501	38.0	37.5	38.0	35.5	38.0
22-23	36.99632238871348	38.0	37.5	38.0	35.5	38.0
24-25	37.20666943476118	38.0	38.0	38.0	36.0	38.0
26-27	37.09773274807907	38.0	38.0	38.0	36.0	38.0
28-29	36.55976118340604	38.0	37.0	38.0	34.0	38.0
30-31	36.88939333102152	38.0	37.0	38.0	35.0	38.0
32-33	36.99434898067201	38.0	37.5	38.0	36.0	38.0
34-35	36.924656877954405	38.0	38.0	38.0	35.5	38.0
36-37	36.88640470150207	38.0	37.5	38.0	35.0	38.0
38-39	36.39506716431531	38.0	37.0	38.0	34.0	38.0
40-41	36.75295440857075	38.0	37.0	38.0	34.5	38.0
42-43	37.097461261886075	38.0	38.0	38.0	36.0	38.0
44-45	36.83407402675622	38.0	37.0	38.0	35.0	38.0
46-47	36.98429714734172	38.0	38.0	38.0	36.0	38.0
48-49	36.57926484276615	38.0	37.0	38.0	34.0	38.0
50-51	36.75423427205198	38.0	37.0	38.0	34.5	38.0
52-53	36.69479704696027	38.0	37.0	38.0	34.5	38.0
54-55	36.70205188808381	38.0	37.0	38.0	34.5	38.0
56-57	36.54632375755142	38.0	37.0	38.0	34.0	38.0
58-59	36.656896205581205	38.0	37.0	38.0	34.5	38.0
60-61	36.285742640214636	38.0	37.0	38.0	34.0	38.0
62-63	36.114302531437644	38.0	37.0	38.0	34.0	38.0
64-65	36.52549004398533	38.0	37.0	38.0	34.0	38.0
66-67	36.273133361318465	38.0	37.0	38.0	33.0	38.0
68-69	36.36882425945867	38.0	37.0	38.0	34.0	38.0
70-71	35.401596521326496	38.0	36.5	38.0	28.5	38.0
72-73	33.64221541859064	38.0	34.5	38.0	21.5	38.0
74-75	33.36592232300926	38.0	34.0	38.0	19.0	38.0
76	33.132927853114566	38.0	34.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	10.0
23	19.0
24	60.0
25	108.0
26	228.0
27	438.0
28	756.0
29	1323.0
30	2274.0
31	3417.0
32	5454.0
33	8578.0
34	14216.0
35	28175.0
36	39016.0
37	115090.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.72185091852014	16.09099375479347	11.100306782075162	37.08684854461123
2	18.22014564435765	16.16917011918016	41.77237137486083	23.838312861601356
3	16.693891332518117	18.482962530342576	39.59546275848223	25.22768337865708
4	12.105090252048695	27.449307367998394	33.47721341096165	26.96838896899126
5	11.983263674691099	30.49999087441368	43.90273950101294	13.61400594988228
6	25.65932361154204	45.04161267361428	16.768721140333266	12.530342574510412
7	24.46159040718366	26.622072968188203	19.007227464364583	29.909109160263547
8	21.992663028599587	43.11018232921465	16.05190633498202	18.84524830720374
9	20.883448011534743	16.092971473417165	16.99366684309467	46.02991367195342
10-11	21.81106386085306	28.980580752313333	28.542324469347157	20.66603091748645
12-13	21.407256666240805	19.522138672409703	29.70127393185012	29.36933072949937
14-15	19.39666186052454	19.87643956124181	20.70139256447227	40.02550601376139
16-17	27.621780949426	29.062482889525654	20.63660090160793	22.679135259440418
18-19	28.004599295504732	23.310853972367724	21.59752514099031	27.08702159113723
20-21	19.901078644302896	21.845512949206984	21.234554945155224	37.018853461334885
22-23	19.810507200087606	30.047133653337227	21.15653118212845	28.985827964446713
24-25	27.44362617607067	29.602531460745933	21.566238672762612	21.387603690420782
26-27	27.609917687211404	30.777180558850908	21.817679910934277	19.795221843003414
28-29	19.89925352703911	30.672464455841286	22.252742238688835	27.175539778430764
30-31	27.451132485262182	23.40416309247869	28.952291434724682	20.19241298753445
32-33	26.549980836268734	29.811693526309064	23.7872095782154	19.851116059206806
34-35	21.250752860871312	29.898614735996787	29.422259130149115	19.42837327298279
36-37	19.438639557591575	29.205754594732714	31.6212972933511	19.734308554324613
38-39	19.015440492051614	23.313819787921375	30.837181288897813	26.8335584311292
40-41	18.830647369093466	20.576828311218996	31.553996094249054	29.038528225438487
42-43	26.6609783585733	20.118587535304773	30.947970232201605	22.272463873920323
44-45	19.007911883338505	28.007793250716357	32.12845175302513	20.855843112920006
46-47	26.240395320399333	20.469830811629645	25.481374678323082	27.808399189647936
48-49	25.169507765873956	19.80092533445274	24.68722052891898	30.34234637075432
50-51	18.87582456948973	20.372428176032894	32.15267684177621	28.599070412701167
52-53	16.636200778192357	20.3156129120597	40.12985086548226	22.91833544426568
54-55	16.106060571478135	20.876366898182493	33.61901914285127	29.398553387488104
56-57	24.021456288518603	20.762383017575896	25.661492809860764	29.55466788404474
58-59	16.828781785898496	20.80604154589813	38.40005842534565	23.96511824285773
60-61	24.370578301154776	28.148934227942856	24.907115797160984	22.573371673741384
62-63	16.83329415818696	37.82333758554825	22.036133937923747	23.307234318341045
64-65	16.356770447103923	38.89975243288878	21.265444422894824	23.47803269711248
66-67	16.419886073176077	38.630595924925146	20.66703790257796	24.28248009932082
68-69	16.87319322535086	39.26407943762107	19.91165485156783	23.95107248546024
70-71	17.323944947429908	38.069509345794394	20.08729738609813	24.51924832067757
72-73	17.952316405839962	34.68652874570423	20.246815540981512	27.114339307474296
74-75	18.477152264238335	33.84230304993942	20.697468004955837	26.983076680866404
76	18.884594146880175	34.71485093391624	20.45945119586001	25.941103723343574
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.5
12	3.0
13	2.5
14	1.5
15	1.5
16	13.5
17	25.0
18	30.0
19	57.5
20	89.0
21	98.0
22	125.5
23	210.5
24	302.0
25	336.0
26	468.0
27	671.5
28	913.5
29	1084.0
30	1240.0
31	1601.0
32	2305.0
33	2804.0
34	3092.0
35	3677.0
36	4685.0
37	5396.0
38	10136.5
39	17869.0
40	19465.0
41	16443.5
42	14818.0
43	13541.0
44	12143.0
45	11616.5
46	11211.0
47	11339.5
48	11117.0
49	10447.0
50	10128.0
51	9778.5
52	8507.0
53	6972.5
54	6360.0
55	5867.5
56	4853.0
57	3848.5
58	3366.0
59	3118.0
60	2701.0
61	2351.5
62	2171.0
63	2067.5
64	1875.5
65	1718.5
66	1621.5
67	1593.0
68	1442.0
69	1237.0
70	1107.5
71	1032.0
72	979.0
73	909.5
74	820.5
75	748.0
76	756.0
77	674.5
78	539.0
79	493.0
80	476.0
81	410.0
82	311.5
83	262.0
84	229.0
85	150.5
86	91.0
87	77.0
88	60.0
89	38.0
90	24.0
91	13.0
92	11.0
93	6.5
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05292840064974175
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.125586318920991E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	4.5627931594604957E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.01574163640013871
52-53	0.030342574510412296
54-55	0.04585607125257798
56-57	0.052015842017849645
58-59	0.03695862459163001
60-61	0.03376466938000767
62-63	0.028517457246628092
64-65	0.01437279845230056
66-67	0.03467722801189976
68-69	0.010722563924732163
70-71	0.012775820846489387
72-73	0.024182803745140626
74-75	0.013003960504462411
76	0.015057217426219635
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	219164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.10689711813984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	38.752418940800126	8.954481575441223
2	13.186682990403224	6.094066543775437
3	12.061135026262786	8.36086218539541
4	10.426128509932466	9.636619152780566
5	7.770230243671261	8.977295541238526
6	5.455945657754433	7.564198499753609
7	3.378618537972434	5.464857367085836
8	2.2195015994628964	4.102863608986877
9	1.3802772402353776	2.8704531766165977
>10	5.26637968484657	20.128762022959975
>50	0.06911259428932506	1.0033582157653629
>100	0.02369574661348288	0.9572740048548118
>500	0.003949291102247146	0.5767370553558067
>1k	0.003949291102247146	2.6044423354200505
>5k	0.0	0.0
>10k+	0.001974645551123573	12.703728714569912
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT	27842	12.703728714569912	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG	3650	1.665419503203081	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	2058	0.9390228322169699	No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT	666	0.303882024420069	Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	598	0.2728550309357376	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT	367	0.16745450895220018	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT	315	0.1437279845230056	No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	272	0.12410797393732546	Illumina PCR Primer Index 10 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0013688379478381486	0.0
2	0.0	0.0	0.0	0.0013688379478381486	0.0
3	0.0	0.0	0.0	0.0013688379478381486	0.0
4	0.0	0.0	0.0	0.003193955211622347	0.0
5	0.0	0.0	0.0	0.005475351791352594	0.0
6	0.0	0.0	0.0	0.005475351791352594	0.0
7	0.0	0.0	0.0	0.005931631107298644	0.0
8	0.0	0.0	0.0	0.005931631107298644	0.0
9	0.0	0.0	0.0	0.005931631107298644	0.0
10	0.0	0.0	0.0	0.005931631107298644	0.0
11	0.0	0.0	0.0	0.006387910423244694	0.0
12	0.0	0.0	0.0	0.007300469055136792	0.0
13	0.0	0.0	0.0	0.007300469055136792	0.0
14	0.0	0.0	0.0	0.01049442426675914	0.0
15	0.0	0.0	0.0	0.015057217426219635	0.0
16	0.0	0.0	0.0	0.015057217426219635	0.0
17	0.0	0.0	0.0	0.015513496742165685	0.0
18	0.0	0.0	0.0	0.015513496742165685	0.0
19	0.0	0.0	0.0	0.02235768648135643	0.0
20	0.0	0.0	0.0	0.023726524429194575	0.0
21	0.0	0.0	0.0	0.025095362377032724	0.0
22	0.0	0.0	0.0	0.02692047964081692	0.0
23	0.0	0.0	0.0	0.03330839006406162	0.0
24	0.0	0.0	0.0	0.03422094869595371	0.0
25	0.0	0.0	0.0	0.04015257980325236	0.0
26	0.0	0.0	0.0	0.04562793159460495	0.0
27	0.0	0.0	0.0	0.09262470113704806	0.0
28	0.0	0.0	0.0	0.13505867752003067	0.0
29	0.0	0.0	0.0	0.18935591611761055	0.0
30	0.0	0.0	0.0	0.24091547881951417	0.0
31	0.0	0.0	0.0	0.29521271741709404	0.0
32	0.0	0.0	0.0	0.3563541457538647	0.0
33	0.0	0.0	0.0	0.44761000894307457	0.0
34	0.0	0.0	0.0	0.5689803069847238	0.0
35	0.0	0.0	0.0	0.7113394535598913	0.0
36	0.0	0.0	0.0	0.9901261156029275	0.0
37	0.0	0.0	0.0	1.4523370626562757	0.0
38	0.0	0.0	0.0	2.090215546348853	0.0
39	0.0	0.0	0.0	2.872734573196328	0.0
40	0.0	0.0	0.0	3.757916446131664	0.0
41	0.0	0.0	0.0	4.754886751473782	0.0
42	0.0	0.0	0.0	5.886003175704039	0.0
43	0.0	0.0	0.0	6.935901881695899	0.0
44	0.0	0.0	0.0	7.96024894599478	0.0
45	0.0	0.0	0.0	9.016991841725831	0.0
46	0.0	0.0	0.0	10.121187786315271	0.0
47	0.0	0.0	0.0	11.193900458104434	0.0
48	0.0	0.0	0.0	12.225091712142506	0.0
49	0.0	0.0	0.0	13.19924805168732	0.0
50	0.0	0.0	0.0	14.05750944498184	0.0
51	0.0	0.0	0.0	14.982387618404482	0.0
52	0.0	0.0	0.0	15.922323009253345	0.0
53	0.0	0.0	0.0	16.77739044733624	0.0
54	0.0	0.0	0.0	17.687211403332665	0.0
55	0.0	0.0	0.0	18.525852786041504	0.0
56	0.0	0.0	0.0	19.25726852950302	0.0
57	0.0	0.0	0.0	20.05301965651293	0.0
58	0.0	0.0	0.0	20.820481465934186	0.0
59	0.0	0.0	0.0	21.551897209395705	0.0
60	0.0	0.0	0.0	22.242704093738023	0.0
61	0.0	0.0	0.0	22.944917960978994	0.0
62	0.0	0.0	0.0	23.679071380336186	0.0
63	0.0	0.0	0.0	24.384022923472834	0.0
64	0.0	0.0	0.0	25.027376758956763	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAC	20	7.914449E-5	69.99977	45
TCGGATT	15	0.0022265508	69.967834	32
CCGAGTT	15	0.0022265508	69.967834	44
TAATACG	20	7.9323676E-5	69.96783	4
GCTTGAC	50	1.4551915E-11	63.100616	56
TCGTTGT	25	2.3921816E-4	55.974262	34
TATATCG	25	2.3921816E-4	55.974262	36
GCGTCTA	25	2.3921816E-4	55.974262	44
TCGTATG	4210	0.0	52.683613	40
CGTATGC	4200	0.0	52.642464	41
CTCGTAT	4190	0.0	52.60111	39
CTTGACA	60	8.367351E-11	52.54781	57
TATGCCG	4195	0.0	52.538418	43
CCTTTGG	20	0.0069258623	52.535805	64
GTCTTCT	4175	0.0	52.51483	49
CAACGCG	20	0.006957204	52.475872	9
AACGCGA	20	0.006957204	52.475872	10
AACTCGT	80	0.0	52.475872	37
GCCGTCT	4165	0.0	52.472733	46
CCGTCTT	4165	0.0	52.38864	47
>>END_MODULE