FastQCFastQC Report
Wed 25 May 2016
SRR1779817_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779817_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92161
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGAC150.002218875869.996248
ATAAGTT150.002218875869.996245
CGCAAGT150.002218875869.996253
AATCGGA207.892163E-569.996210
ACGAATA252.8212053E-669.996224
ATCTCGG950.055.2601642
TCGGACG452.6684575E-854.4414912
ATCGGAC452.6684575E-854.4414911
ATCTAGT200.00691847552.5256531
GGCAGAT200.00693335252.4971512
GTGGCCG200.00693335252.4971548
TGGCCGC200.00693335252.4971549
TATGACT200.00693335252.4971549
GTGGACT200.00693335252.4971520
CTCGGTG800.052.4971544
TCTCGGT800.052.4971543
CGGTGGA200.00693335252.4971546
TATAGAG200.00693335252.497153
GTAAGCG200.00693335252.4971548
GACGAAT407.411563E-752.4971523
Produced by FastQC (version 0.11.5)