##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779817_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92161
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.749601241305974	32.0	21.0	34.0	12.0	34.0
2	26.993250941287528	32.0	21.0	34.0	12.0	34.0
3	26.90945193737047	33.0	21.0	34.0	11.0	34.0
4	27.22328316750035	32.0	21.0	34.0	12.0	34.0
5	27.57629583012337	34.0	23.0	34.0	11.0	34.0
6	29.635214461648637	35.0	23.0	38.0	11.0	38.0
7	29.952724037282582	36.0	23.0	38.0	12.0	38.0
8	29.225789650719936	34.0	21.0	38.0	11.0	38.0
9	30.169768123175746	36.0	24.0	38.0	11.0	38.0
10-11	28.78068814357483	34.0	21.0	38.0	11.0	38.0
12-13	29.377572943001923	35.0	22.0	38.0	11.0	38.0
14-15	28.36600622823103	34.0	21.0	38.0	11.0	38.0
16-17	28.844386454140036	35.0	21.5	38.0	11.0	38.0
18-19	29.43579171232951	36.0	21.0	38.0	11.0	38.0
20-21	28.51054133527197	33.5	21.5	38.0	11.0	38.0
22-23	29.202140818784518	35.0	22.0	38.0	11.0	38.0
24-25	29.498459218107442	35.5	22.0	38.0	11.0	38.0
26-27	28.502246069378586	34.0	21.0	38.0	11.0	38.0
28-29	29.422917503065285	35.5	21.0	38.0	11.0	38.0
30-31	29.773635268714532	37.0	22.0	38.0	11.0	38.0
32-33	29.97316652380074	37.0	23.0	38.0	11.0	38.0
34-35	29.93327437853322	37.0	23.0	38.0	11.0	38.0
36-37	29.447054610952573	36.0	21.5	38.0	11.0	38.0
38-39	29.382054231182387	36.0	21.5	38.0	11.0	38.0
40-41	29.34180944217185	35.5	21.0	38.0	11.0	38.0
42-43	29.69527782901661	36.5	22.0	38.0	11.0	38.0
44-45	29.76701099163421	36.0	22.5	38.0	11.0	38.0
46-47	29.68134026323499	36.0	22.0	38.0	11.0	38.0
48-49	29.332581026681567	35.5	21.5	38.0	11.0	38.0
50-51	28.75682230010525	35.0	21.0	38.0	11.0	38.0
52-53	29.287681340263234	35.0	21.5	38.0	11.0	38.0
54-55	29.41237616779332	35.5	21.5	38.0	11.0	38.0
56-57	29.44945801369343	36.0	21.5	38.0	11.0	38.0
58-59	29.642023198533003	36.0	21.5	38.0	11.0	38.0
60-61	29.604398823797485	36.0	22.0	38.0	11.0	38.0
62-63	29.532345569167003	36.0	21.5	38.0	11.0	38.0
64-65	29.547162031661983	36.5	21.5	38.0	11.0	38.0
66-67	29.540212237280414	36.0	22.0	38.0	11.0	38.0
68-69	28.970589511832554	34.5	21.0	38.0	11.0	38.0
70-71	29.0539056650861	34.5	21.5	38.0	11.0	38.0
72-73	29.10489795032606	35.0	21.0	38.0	11.0	38.0
74-75	29.255661288397476	35.5	21.5	38.0	11.0	38.0
76	28.417671249226895	34.0	21.0	37.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	32.0
13	470.0
14	1511.0
15	2599.0
16	3536.0
17	4380.0
18	4524.0
19	4215.0
20	3333.0
21	2483.0
22	1903.0
23	1578.0
24	1372.0
25	1287.0
26	1334.0
27	1460.0
28	1591.0
29	1832.0
30	2074.0
31	2541.0
32	2940.0
33	3853.0
34	4925.0
35	6940.0
36	11371.0
37	18073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.6447521185751	18.727010340599605	9.377068391184991	32.251169149640305
2	18.451405692212543	21.92901552717527	29.117522596325994	30.502056184286197
3	16.80971343626914	20.063801391043935	35.14067772702119	27.98580744566574
4	12.358806870585171	29.714304315274358	26.565466954568635	31.361421859571838
5	13.217087488199997	32.02113692342748	34.82492594481397	19.936849643558556
6	22.693634844506175	38.309208099134096	16.546583042166713	22.450574014193016
7	22.584285512766257	32.61282376813482	16.933059886932085	27.869830832166848
8	19.983723958333332	37.80381944444444	15.520833333333334	26.691623263888886
9	21.39113450165482	19.370625576474417	17.061472519125388	42.17676740274538
10-11	20.363287162877	29.294639127155932	22.36364524162177	27.978428468345296
12-13	20.495654344028384	23.265264048784193	25.67137943381691	30.567702173370513
14-15	25.076361347865948	25.993240054036164	20.480032117881304	28.450366480216577
16-17	26.04206791412807	25.753441007807034	20.526689850858016	27.67780122720688
18-19	20.9148270118653	25.770539119678382	24.16300001627613	29.151633852180186
20-21	24.74799535595317	24.7859723744317	22.55346621672942	27.912566052885712
22-23	25.60112847222222	24.56488715277778	21.4501953125	28.383789062499996
24-25	20.75825886923073	29.2957242142613	21.093008241236568	28.853008675271408
26-27	23.601217441311626	29.329810493649667	22.168391013503616	24.900581051535095
28-29	21.163340820556225	28.21652180518029	24.02964506223076	26.590492312032726
30-31	22.186174195158472	27.133494645240397	24.154468810017253	26.52586234958388
32-33	19.940647345406408	28.692179989366434	24.077972244224778	27.289200421002374
34-35	19.673065028917414	30.03233542030621	23.66724899358717	26.627350557189207
36-37	23.737587497965162	26.048619024363774	23.615497314015954	26.59829616365511
38-39	23.735269223256722	24.54207088135078	26.001584304533715	25.721075590858778
40-41	20.893983865104897	28.14600615231037	24.213735819575632	26.746274163009097
42-43	18.591478995882184	27.436374585641353	25.57006526657299	28.402081151903474
44-45	18.221079245201125	24.91997352344369	27.9571817658995	28.901765465455693
46-47	20.31478035362605	26.23549389916395	25.57034272104341	27.879383026166593
48-49	20.753355540846997	27.05374290643548	25.01274942763208	27.18015212508545
50-51	24.664438633231697	26.109766816046182	22.84801267374863	26.377781876973494
52-53	17.4401128564065	28.377417867122436	25.077453134749465	29.105016141721602
54-55	19.12525908000825	26.461970852820855	24.79300728136903	29.619762785801875
56-57	20.346616312887964	27.321265789816383	23.497525719494725	28.83459217780093
58-59	19.112859468258275	27.87194791101465	24.244167118827995	28.77102550189908
60-61	17.982429961311187	26.46900825325165	24.553016696420336	30.99554508901683
62-63	19.254058512023615	26.849118847122146	24.539890615504817	29.356932025349426
64-65	19.46180894705997	26.03318790562393	23.883245425538842	30.621757721777254
66-67	18.09001871998698	30.422420575707427	21.816652649285114	29.670908055020483
68-69	18.517252604166668	31.993815104166668	21.03732638888889	28.45160590277778
70-71	17.66429218591479	30.806039463761593	21.910688418574118	29.6189799317495
72-73	17.844993625044083	30.199386919133005	22.836448471366953	29.119170984455963
74-75	17.896672960479197	28.966187034746188	22.379386678820236	30.75775332595438
76	16.909905706442128	28.324960123265225	21.636519493484087	33.12861467680856
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	0.5
12	0.0
13	0.5
14	4.5
15	10.0
16	13.5
17	15.0
18	20.0
19	26.0
20	34.0
21	41.0
22	68.0
23	116.0
24	183.5
25	230.0
26	271.0
27	348.0
28	482.0
29	580.0
30	615.5
31	762.0
32	1067.0
33	1261.0
34	1443.0
35	1814.5
36	2167.5
37	2331.0
38	2556.5
39	3003.0
40	3443.5
41	3961.5
42	4260.0
43	4442.0
44	4786.5
45	5084.5
46	5220.0
47	5257.0
48	5253.5
49	5119.5
50	5026.0
51	4833.5
52	4508.0
53	4194.5
54	4014.0
55	3761.5
56	3300.0
57	2914.0
58	2737.0
59	2522.0
60	2155.5
61	1786.0
62	1568.0
63	1475.5
64	1296.0
65	1106.5
66	953.0
67	902.0
68	793.0
69	657.5
70	577.0
71	523.0
72	495.0
73	438.0
74	359.5
75	310.0
76	323.0
77	307.5
78	246.0
79	213.0
80	209.5
81	162.5
82	101.0
83	83.0
84	79.5
85	61.5
86	49.5
87	52.0
88	40.5
89	25.0
90	19.5
91	19.5
92	21.0
93	13.0
94	5.5
95	4.5
96	3.0
97	2.5
98	3.5
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0032551730124456115
7	0.004340230683260815
8	0.0010850576708152037
9	0.006510346024891223
10-11	0.0027126441770380094
12-13	0.0
14-15	5.425288354076018E-4
16-17	5.425288354076018E-4
18-19	0.0016275865062228057
20-21	0.0
22-23	0.0010850576708152037
24-25	0.0027126441770380094
26-27	5.425288354076018E-4
28-29	0.004340230683260815
30-31	0.0
32-33	0.0
34-35	0.0021701153416304074
36-37	0.01736092273304326
38-39	0.007595403695706426
40-41	5.425288354076018E-4
42-43	5.425288354076018E-4
44-45	0.004340230683260815
46-47	0.0016275865062228057
48-49	0.0
50-51	0.0021701153416304074
52-53	0.009222990201929232
54-55	0.00868046136652163
56-57	0.01410574972059765
58-59	0.011935634378967242
60-61	0.01681839389763566
62-63	0.009765519037336834
64-65	0.020616095745488873
66-67	0.014648278556005253
68-69	0.0010850576708152037
70-71	5.425288354076018E-4
72-73	0.003797701847853213
74-75	0.007595403695706426
76	0.0021701153416304074
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	92161.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.27256648690879
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.4668829413355	70.21191176311021
2	6.382806163828062	10.247284643178785
3	3.13463098134631	7.548746215861373
4	1.6031359826980265	5.147513590347327
5	0.7366855907001892	2.9567821529714307
6	0.3433360367666937	1.6536278903223707
7	0.17166801838334686	0.9646162693547162
8	0.06217896728845634	0.39930122285999503
9	0.0364963503649635	0.2636690140080945
>10	0.06217896728845634	0.606547237985699
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004340230683260816	0.0
2	0.0	0.0	0.0	0.004340230683260816	0.0
3	0.0	0.0	0.0	0.004340230683260816	0.0
4	0.0	0.0	0.0	0.005425288354076019	0.0
5	0.0	0.0	0.0	0.009765519037336835	0.0
6	0.0	0.0	0.0	0.009765519037336835	0.0
7	0.0	0.0	0.0	0.009765519037336835	0.0
8	0.0	0.0	0.0	0.010850576708152038	0.0
9	0.0	0.0	0.0	0.017360922733043262	0.0
10	0.0	0.0	0.0	0.020616095745488873	0.0
11	0.0	0.0	0.0	0.021701153416304075	0.0
12	0.0	0.0	0.0	0.02278621108711928	0.0
13	0.0	0.0	0.0	0.027126441770380094	0.0
14	0.0	0.0	0.0	0.027126441770380094	0.0
15	0.0	0.0	0.0	0.029296557112010502	0.0
16	0.0	0.0	0.0	0.030381614782825708	0.0
17	0.0	0.0	0.0	0.030381614782825708	0.0
18	0.0	0.0	0.0	0.030381614782825708	0.0
19	0.0	0.0	0.0	0.030381614782825708	0.0
20	0.0	0.0	0.0	0.030381614782825708	0.0
21	0.0	0.0	0.0	0.03580690313690173	0.0
22	0.0	0.0	0.0	0.04991265285749938	0.0
23	0.0	0.0	0.0	0.053167825869944986	0.0
24	0.0	0.0	0.0	0.07269886394461865	0.0
25	0.0	0.0	0.0	0.07378392161543386	0.0
26	0.0	0.0	0.0	0.07378392161543386	0.0
27	0.0	0.0	0.0	0.09548507503173793	0.0
28	0.0	0.0	0.0	0.10959082475233559	0.0
29	0.0	0.0	0.0	0.1421425548767917	0.0
30	0.0	0.0	0.0	0.17686440034287823	0.0
31	0.0	0.0	0.0	0.2213517648463016	0.0
32	0.0	0.0	0.0	0.2756046483870618	0.0
33	0.001085057670815204	0.0	0.0	0.30381614782825705	0.0
34	0.001085057670815204	0.0	0.0	0.39930122285999503	0.0
35	0.001085057670815204	0.0	0.0	0.4763403174878745	0.0
36	0.001085057670815204	0.0	0.0	0.6184828723646661	0.0
37	0.001085057670815204	0.0	0.0	0.8604507329564567	0.0
38	0.001085057670815204	0.0	0.0	1.1696921691387898	0.0
39	0.001085057670815204	0.0	0.0	1.4452968175258516	0.0
40	0.001085057670815204	0.0	0.0	1.8272371176528033	0.0
41	0.001085057670815204	0.0	0.0	2.1907314373758964	0.0
42	0.001085057670815204	0.0	0.0	2.588947602565076	0.0
43	0.001085057670815204	0.0	0.0	3.0056097481581148	0.0
44	0.001085057670815204	0.0	0.0	3.5264374301494126	0.0
45	0.001085057670815204	0.0	0.0	4.04943522748234	0.0
46	0.001085057670815204	0.0	0.0	4.546391640715704	0.0
47	0.001085057670815204	0.0	0.0	5.055283688328035	0.0
48	0.001085057670815204	0.0	0.0	5.48171135295841	0.0
49	0.001085057670815204	0.0	0.0	5.918989594296937	0.0
50	0.001085057670815204	0.0	0.0	6.335651739889975	0.0
51	0.001085057670815204	0.0	0.0	6.715421924675296	0.0
52	0.001085057670815204	0.0	0.0	7.163550742721975	0.0
53	0.001085057670815204	0.0	0.0	7.697399116763056	0.0
54	0.001085057670815204	0.0	0.0	8.167229088226039	0.0
55	0.001085057670815204	0.0	0.0	8.62620848298087	0.0
56	0.001085057670815204	0.0	0.0	9.059146493636137	0.0
57	0.001085057670815204	0.0	0.0	9.462787947179393	0.0
58	0.001085057670815204	0.0	0.0	9.854493766343682	0.0
59	0.001085057670815204	0.0	0.0	10.238604181812264	0.0
60	0.001085057670815204	0.0	0.0	10.581482405789867	0.0
61	0.001085057670815204	0.0	0.0	10.98837903234557	0.0
62	0.001085057670815204	0.0	0.0	11.320406679615022	0.0
63	0.001085057670815204	0.0	0.0	11.683900999338114	0.0
64	0.001085057670815204	0.0	0.0	12.022438992632459	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATGAC	15	0.0022188758	69.9962	48
ATAAGTT	15	0.0022188758	69.9962	45
CGCAAGT	15	0.0022188758	69.9962	53
AATCGGA	20	7.892163E-5	69.9962	10
ACGAATA	25	2.8212053E-6	69.9962	24
ATCTCGG	95	0.0	55.26016	42
TCGGACG	45	2.6684575E-8	54.44149	12
ATCGGAC	45	2.6684575E-8	54.44149	11
ATCTAGT	20	0.006918475	52.52565	31
GGCAGAT	20	0.006933352	52.49715	12
GTGGCCG	20	0.006933352	52.49715	48
TGGCCGC	20	0.006933352	52.49715	49
TATGACT	20	0.006933352	52.49715	49
GTGGACT	20	0.006933352	52.49715	20
CTCGGTG	80	0.0	52.49715	44
TCTCGGT	80	0.0	52.49715	43
CGGTGGA	20	0.006933352	52.49715	46
TATAGAG	20	0.006933352	52.49715	3
GTAAGCG	20	0.006933352	52.49715	48
GACGAAT	40	7.411563E-7	52.49715	23
>>END_MODULE