FastQCFastQC Report
Wed 25 May 2016
SRR1779817_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779817_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92161
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT2018421.900804027734075No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG24902.7017936003298577No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC15061.6340968522476969No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT5500.5967817189483621RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC4530.4915311248792873No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT3450.3743448964312453No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT3090.335282820281898No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC2040.2213517648463016No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT1470.15950347760983496No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1410.15299313158494374No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT1210.13129197816863966No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG1180.12803680515619406No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC1130.12261151680211804RNA PCR Primer, Index 24 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG1100.11935634378967243No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTATG150.00221498670.02714549
AGACTGG150.002219762469.9891536
ACTAGAG150.002219762469.989153
TAGGGAA150.002219762469.989155
TCCGGGC150.002219762469.989158
TCTTTAT509.622454E-1056.0217171
CCGTGAT252.3812968E-455.99131827
ATCTCGT28650.053.86599737
AACTCGT653.6379788E-1253.83780737
CAACTCG653.6379788E-1253.83780736
TCTCGTA28550.053.80952538
CATCTCG28600.053.71544336
CTCGTAT29950.053.63108439
GCATCTC28650.053.62170435
TAGCATC28600.053.59308633
AGCATCT28600.053.59308634
CCGTCTT29950.053.5142447
CGTATGC30300.053.47355741
TCTTCTG30200.053.4478750
CGTCTTC29950.053.42638448