##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779817_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	92161
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.52850989030067	34.0	34.0	34.0	34.0	34.0
2	33.623083516888926	34.0	34.0	34.0	34.0	34.0
3	33.744664228903765	34.0	34.0	34.0	34.0	34.0
4	33.51542409479064	34.0	34.0	34.0	34.0	34.0
5	33.59322272978809	34.0	34.0	34.0	34.0	34.0
6	37.24749080413624	38.0	38.0	38.0	36.0	38.0
7	37.30536778029752	38.0	38.0	38.0	37.0	38.0
8	37.2000520827682	38.0	38.0	38.0	36.0	38.0
9	37.11179349182409	38.0	38.0	38.0	36.0	38.0
10-11	37.05920074651968	38.0	37.5	38.0	35.5	38.0
12-13	37.243047492974256	38.0	38.0	38.0	36.5	38.0
14-15	37.260782760603725	38.0	38.0	38.0	36.0	38.0
16-17	37.13055956424084	38.0	38.0	38.0	36.0	38.0
18-19	36.981640824209805	38.0	38.0	38.0	35.5	38.0
20-21	37.01866299193802	38.0	37.5	38.0	35.5	38.0
22-23	37.02478814248977	38.0	37.5	38.0	35.5	38.0
24-25	37.18046136652163	38.0	38.0	38.0	36.0	38.0
26-27	37.069150725361055	38.0	38.0	38.0	36.0	38.0
28-29	36.493999631080385	38.0	37.0	38.0	34.0	38.0
30-31	36.74353576892612	38.0	37.0	38.0	34.5	38.0
32-33	36.883529909614694	38.0	37.0	38.0	35.5	38.0
34-35	36.93167391846877	38.0	38.0	38.0	35.5	38.0
36-37	36.84710452360543	38.0	37.5	38.0	35.0	38.0
38-39	35.54886557220516	38.0	36.5	38.0	30.5	38.0
40-41	36.42203860635193	38.0	37.0	38.0	34.0	38.0
42-43	37.059944011024186	38.0	37.5	38.0	36.0	38.0
44-45	36.789395731383124	38.0	37.0	38.0	35.0	38.0
46-47	36.95415631340806	38.0	37.5	38.0	35.5	38.0
48-49	36.52264515358991	38.0	37.0	38.0	34.0	38.0
50-51	36.641806186998835	38.0	37.0	38.0	34.0	38.0
52-53	36.55722051627044	38.0	37.0	38.0	34.0	38.0
54-55	36.60239146710647	38.0	37.0	38.0	34.0	38.0
56-57	36.44478141513221	38.0	37.0	38.0	34.0	38.0
58-59	36.49689673506147	38.0	37.0	38.0	34.0	38.0
60-61	36.07372966873189	38.0	37.0	38.0	34.0	38.0
62-63	35.969645511658946	38.0	37.0	38.0	33.0	38.0
64-65	36.46551144193314	38.0	37.0	38.0	34.0	38.0
66-67	36.161499983724134	38.0	37.0	38.0	32.5	38.0
68-69	36.14023285337616	38.0	37.0	38.0	33.5	38.0
70-71	34.38104512754853	38.0	34.0	38.0	26.0	38.0
72-73	31.26218248499908	37.5	24.0	38.0	11.0	38.0
74-75	30.73541953754842	37.5	23.5	38.0	11.0	38.0
76	30.6170614468159	37.0	24.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	2.0
22	6.0
23	3.0
24	23.0
25	65.0
26	124.0
27	227.0
28	414.0
29	663.0
30	1133.0
31	1777.0
32	2728.0
33	4343.0
34	7551.0
35	15318.0
36	17460.0
37	40323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.41331104695335	13.28065643519222	11.32155338962815	44.984479128226276
2	15.159340718959214	14.154577315784334	49.47754473150248	21.208537233753972
3	14.43343713718384	15.872223608684802	48.50641811612287	21.187921138008488
4	10.414383524484325	35.96857672985319	30.73859875652391	22.878440989138575
5	10.149629452805417	27.513807358861126	50.40743915539111	11.929124032942351
6	20.975249834528707	51.589066958908866	16.06753398943154	11.36814921713089
7	19.737199032128558	24.89013791082996	16.921474376363104	38.45118868067838
8	17.99242629745771	50.40852421306192	14.010264645565911	17.588784843914453
9	17.19599396707935	16.04149260533197	14.204489968641834	52.55802345894685
10-11	17.871442367161812	32.10360130640944	30.60730677835527	19.417649548073477
12-13	18.08357114180619	16.57425592170224	33.558663642972625	31.783509293518954
14-15	16.58510649841039	16.681676631112943	19.000444873645034	47.73277199683163
16-17	30.571499875218365	30.746194160219613	16.913878972667398	21.768426991894618
18-19	31.250745977148686	21.68216490706481	17.713023947222794	29.354065168563707
20-21	17.813391781773202	18.2056401297729	17.567626219333558	46.413341869120345
22-23	16.751662850880525	32.336346176799296	17.77324464795304	33.13874632436714
24-25	30.257703993055557	32.23687065972222	17.745768229166668	19.759657118055557
26-27	31.619123056390446	33.85813956011762	18.049934354010915	16.472803029481017
28-29	29.941623897310144	21.543277525200462	18.660279293844468	29.85481928364492
30-31	31.762350668938055	18.44489534618765	32.54304966308959	17.249704321784705
32-33	19.14312995735723	32.56420828767049	31.54805177895205	16.744609976020225
34-35	28.194681047297664	33.38342682913597	21.896463797050814	16.525428326515552
36-37	19.011295450353185	31.82799665802237	18.51922179663849	30.641486094985947
38-39	16.192858150410693	21.407645316348564	31.95983116502642	30.43966536821432
40-41	15.699699439025183	17.796573387875565	34.50212128774645	32.00160588535281
42-43	30.258624898953457	17.423950607906857	32.69079486330912	19.626629629830568
44-45	16.428858193813	31.546424192445826	34.77501329195647	17.249704321784705
46-47	29.682837642820715	18.396067751000967	22.677162791202353	29.243931814975966
48-49	28.572281116741355	17.249704321784705	19.62760820737622	34.55040635409772
50-51	18.046956814272612	17.263981508109193	33.62181695849634	31.06724471912185
52-53	14.59316901637743	17.51592702330175	47.65571582066226	20.235188139658558
54-55	14.403651798217524	17.406289690509016	36.7291221137876	31.46093639748586
56-57	28.598412887957707	17.447376705711214	22.71187728649435	31.24233311983673
58-59	15.563081030917763	18.28473431996657	45.662449867851926	20.48973478126374
60-61	28.283957518166574	30.845457949605738	23.192432719911867	17.678151812315825
62-63	15.450569248635215	47.70021380739969	18.701092913966942	18.148124029998154
64-65	14.259823976906464	49.65110095825149	17.829663472495035	18.25941159234701
66-67	14.370994263758568	49.84343371303597	17.552247553821356	18.233324469384097
68-69	14.31439252133622	49.76371459109874	17.66687826554031	18.255014622024728
70-71	14.950786226654659	47.22406103159014	17.765792358028847	20.059360383726357
72-73	16.141950187204948	40.59580009767215	18.95382277931521	24.308426935807695
74-75	17.014915655198234	38.58537299989691	19.62040769819265	24.779303646712208
76	17.146670717944264	38.93241600833406	19.345212258008505	24.575701015713168
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.5
14	2.0
15	3.5
16	11.0
17	16.0
18	16.0
19	22.5
20	47.0
21	65.0
22	85.0
23	130.5
24	179.5
25	203.0
26	260.5
27	395.0
28	525.5
29	579.0
30	665.5
31	793.0
32	1038.5
33	1243.0
34	1316.0
35	1571.0
36	1927.0
37	2101.0
38	2213.0
39	2669.0
40	6220.5
41	10981.0
42	12534.0
43	11460.0
44	8792.0
45	6096.5
46	4995.0
47	4539.0
48	3809.0
49	3206.5
50	2878.0
51	2827.0
52	2475.5
53	2148.5
54	2122.0
55	1995.5
56	1774.0
57	1611.0
58	1543.0
59	1406.5
60	1193.5
61	1021.5
62	926.0
63	916.0
64	782.0
65	676.5
66	649.5
67	604.0
68	571.0
69	512.5
70	447.5
71	408.0
72	374.0
73	303.5
74	257.0
75	247.0
76	232.5
77	210.0
78	186.0
79	170.0
80	162.5
81	138.5
82	95.5
83	69.0
84	66.5
85	59.5
86	41.5
87	28.0
88	24.0
89	13.5
90	6.5
91	4.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029296557112010506
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0010850576708152037
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	5.425288354076018E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.013563220885190049
52-53	0.023871268757934484
54-55	0.04557242217423856
56-57	0.04774253751586897
58-59	0.032009201289048515
60-61	0.029839085947418107
62-63	0.023871268757934484
64-65	0.015190807391412852
66-67	0.029839085947418107
68-69	0.005967817189483621
70-71	0.013020692049782446
72-73	0.01736092273304326
74-75	0.010308047872744436
76	0.009765519037336834
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	92161.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.27268584325257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	48.66928608495812	14.246807217803624
2	17.46237675142709	10.22341337442085
3	12.665875898880568	11.122926183526655
4	8.34754244198977	9.774199498703357
5	5.367336348135518	7.855817536702076
6	2.961672473867596	5.201766473888087
7	1.8867225146415596	3.8660604811145713
8	0.9229742753354584	2.161434880263886
9	0.5893691155756543	1.5527175269365567
>10	1.0564163392393804	4.511669795249618
>50	0.018533619986655793	0.41340697258059267
>100	0.037067239973311586	2.236303859550135
>500	0.003706723997331159	0.5967817189483621
>1k	0.007413447994662318	4.335890452577554
>5k	0.0	0.0
>10k+	0.003706723997331159	21.900804027734075
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT	20184	21.900804027734075	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	2490	2.7017936003298577	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	1506	1.6340968522476969	No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT	550	0.5967817189483621	RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC	453	0.4915311248792873	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCGT	345	0.3743448964312453	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAACTCGTATGCCGT	309	0.335282820281898	No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	204	0.2213517648463016	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCAGCTCGTATGCCGT	147	0.15950347760983496	No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	141	0.15299313158494374	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATATCGTATGCCGT	121	0.13129197816863966	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCACCTCGTATGCCG	118	0.12803680515619406	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC	113	0.12261151680211804	RNA PCR Primer, Index 24 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG	110	0.11935634378967243	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0032551730124456115	0.0
2	0.0	0.0	0.0	0.0032551730124456115	0.0
3	0.0	0.0	0.0	0.0032551730124456115	0.0
4	0.0	0.0	0.0	0.004340230683260816	0.0
5	0.0	0.0	0.0	0.008680461366521631	0.0
6	0.0	0.0	0.0	0.008680461366521631	0.0
7	0.0	0.0	0.0	0.008680461366521631	0.0
8	0.0	0.0	0.0	0.009765519037336835	0.0
9	0.0	0.0	0.0	0.016275865062228057	0.0
10	0.0	0.0	0.0	0.01953103807467367	0.0
11	0.0	0.0	0.0	0.020616095745488873	0.0
12	0.0	0.0	0.0	0.021701153416304075	0.0
13	0.0	0.0	0.0	0.027126441770380094	0.0
14	0.0	0.0	0.0	0.0282114994411953	0.0
15	0.0	0.0	0.0	0.030381614782825708	0.0
16	0.0	0.0	0.0	0.03146667245364091	0.0
17	0.0	0.0	0.0	0.03146667245364091	0.0
18	0.0	0.0	0.0	0.03146667245364091	0.0
19	0.0	0.0	0.0	0.03146667245364091	0.0
20	0.0	0.0	0.0	0.033636787795271315	0.0
21	0.0	0.0	0.0	0.033636787795271315	0.0
22	0.0	0.0	0.0	0.034721845466086525	0.0
23	0.0	0.0	0.0	0.04557242217423856	0.0
24	0.0	0.0	0.0	0.052082768199129784	0.0
25	0.0	0.0	0.0	0.060763229565651417	0.0
26	0.0	0.0	0.0	0.06184828723646662	0.0
27	0.0	0.0	0.0	0.0835494406527707	0.0
28	0.0	0.0	0.0	0.09982530571499876	0.0
29	0.0	0.0	0.0	0.13563220885190047	0.0
30	0.0	0.0	0.0	0.1736092273304326	0.0
31	0.0	0.0	0.0	0.22243682251711677	0.0
32	0.0	0.0	0.0	0.2832000520827682	0.0
33	0.0	0.0	0.0	0.3168368398780395	0.0
34	0.0	0.0	0.0	0.4210023762762991	0.0
35	0.0	0.0	0.0	0.5099771052831458	0.0
36	0.0	0.0	0.0	0.6662254098805351	0.0
37	0.0	0.0	0.0	0.9700415577087922	0.0
38	0.0	0.0	0.0	1.2868783975868316	0.0
39	0.0	0.0	0.0	1.580929026377752	0.0
40	0.0	0.0	0.0	1.9899957682750837	0.0
41	0.0	0.0	0.0	2.383871702781003	0.0
42	0.0	0.0	0.0	2.8189798287778998	0.0
43	0.0	0.0	0.0	3.2584281854580572	0.0
44	0.0	0.0	0.0	3.833508750990115	0.0
45	0.0	0.0	0.0	4.394483566801576	0.0
46	0.0	0.0	0.0	4.941352632892438	0.0
47	0.0	0.0	0.0	5.494732045008192	0.0
48	0.0	0.0	0.0	5.9667321318128055	0.0
49	0.0	0.0	0.0	6.437647160946605	0.0
50	0.0	0.0	0.0	6.899881728713881	0.0
51	0.0	0.0	0.0	7.306778355269582	0.0
52	0.0	0.0	0.0	7.755992230987077	0.0
53	0.0	0.0	0.0	8.295265893382233	0.0
54	0.0	0.0	0.0	8.76184069183277	0.0
55	0.0	0.0	0.0	9.222990201929232	0.0
56	0.0	0.0	0.0	9.673289135317543	0.0
57	0.0	0.0	0.0	10.084525992556504	0.0
58	0.0	0.0	0.0	10.491422619112205	0.0
59	0.0	0.0	0.0	10.880958322934864	0.0
60	0.0	0.0	0.0	11.244452642657958	0.0
61	0.0	0.0	0.0	11.671965364959147	0.0
62	0.0	0.0	0.0	12.013758531265937	0.0
63	0.0	0.0	0.0	12.40220917741778	0.0
64	0.0	0.0	0.0	12.764618439470057	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTATG	15	0.002214986	70.027145	49
AGACTGG	15	0.0022197624	69.98915	36
ACTAGAG	15	0.0022197624	69.98915	3
TAGGGAA	15	0.0022197624	69.98915	5
TCCGGGC	15	0.0022197624	69.98915	8
TCTTTAT	50	9.622454E-10	56.021717	1
CCGTGAT	25	2.3812968E-4	55.991318	27
ATCTCGT	2865	0.0	53.865997	37
AACTCGT	65	3.6379788E-12	53.837807	37
CAACTCG	65	3.6379788E-12	53.837807	36
TCTCGTA	2855	0.0	53.809525	38
CATCTCG	2860	0.0	53.715443	36
CTCGTAT	2995	0.0	53.631084	39
GCATCTC	2865	0.0	53.621704	35
TAGCATC	2860	0.0	53.593086	33
AGCATCT	2860	0.0	53.593086	34
CCGTCTT	2995	0.0	53.51424	47
CGTATGC	3030	0.0	53.473557	41
TCTTCTG	3020	0.0	53.44787	50
CGTCTTC	2995	0.0	53.426384	48
>>END_MODULE