FastQCFastQC Report
Wed 25 May 2016
SRR1779816_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779816_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66115
Sequences flagged as poor quality0
Sequence length76
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTG301.0055555E-770.03860532
CTTACGT252.802246E-670.03860531
CCTTACG301.0108488E-769.9856330
TCGGGGG150.002217419269.9856345
CATCTCG150.002217419269.9856341
TGGCCGC207.881563E-569.9856349
GTTACTC150.002217419269.9856326
TCTCGCT207.881563E-569.9856343
ACGAGGG150.002217419269.9856324
AGTGTAG650.064.65102435
TTAGATC401.0482836E-861.23742338
TAGTGTA352.9217517E-760.03309234
TGATACA352.9371222E-759.9876822
TACGTGT308.266283E-658.365533
ATCTCGC308.30351E-658.32135442
GGCGGTC308.30351E-658.32135447
CAGTGTA252.368058E-456.03088434
GCGGTCG252.3769416E-455.98850348
CGGTGGC252.3769416E-455.98850346
CGGTCGC252.3769416E-455.98850349
Produced by FastQC (version 0.11.5)