##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779816_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66115
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.66559782197686	23.0	12.0	34.0	12.0	34.0
2	24.122952431369583	23.0	12.0	34.0	12.0	34.0
3	23.951085230280572	23.0	11.0	34.0	11.0	34.0
4	24.57918777887015	23.0	12.0	34.0	12.0	34.0
5	23.887453679195342	23.0	11.0	34.0	11.0	34.0
6	25.82450276034183	27.0	12.0	37.0	11.0	38.0
7	26.145625047266126	28.0	12.0	37.0	11.0	38.0
8	26.151629736065946	28.0	11.0	37.0	11.0	38.0
9	26.640611056492475	31.0	12.0	37.0	11.0	38.0
10-11	25.534500491567723	27.0	11.5	37.0	11.0	38.0
12-13	25.77362171973077	27.5	11.0	37.0	11.0	38.0
14-15	24.989344324283444	26.5	11.0	37.0	11.0	38.0
16-17	25.187151175981242	26.5	11.0	37.0	11.0	38.0
18-19	25.355857218482946	26.5	11.0	37.0	11.0	38.0
20-21	25.0225364894502	26.5	11.0	36.5	11.0	38.0
22-23	25.386795734704684	27.0	11.0	37.0	11.0	38.0
24-25	25.695825455645465	27.0	11.0	37.0	11.0	38.0
26-27	24.818392195417076	26.0	11.0	37.0	11.0	38.0
28-29	25.19884292520608	26.0	11.0	37.0	11.0	38.0
30-31	25.333721545791423	26.0	11.0	37.5	11.0	38.0
32-33	25.600453754821146	27.0	11.0	38.0	11.0	38.0
34-35	25.629410874990548	27.0	11.0	37.5	11.0	38.0
36-37	25.277629887317552	26.0	11.0	37.0	11.0	38.0
38-39	25.156666414580656	26.0	11.0	37.0	11.0	38.0
40-41	25.13909854042199	26.0	11.0	37.0	11.0	38.0
42-43	25.412750510474176	26.5	11.0	37.0	11.0	38.0
44-45	25.47862814792407	27.0	11.0	37.0	11.0	38.0
46-47	25.39694471753762	26.5	11.0	37.0	11.0	38.0
48-49	25.040830371322695	26.0	11.0	37.0	11.0	38.0
50-51	24.645367919534145	25.5	11.0	37.0	11.0	38.0
52-53	25.151002041896696	26.0	11.0	37.0	11.0	38.0
54-55	25.14660062013159	26.0	11.0	37.0	11.0	38.0
56-57	25.196997655600093	26.0	11.0	37.0	11.0	38.0
58-59	25.137646524994327	26.0	11.0	37.0	11.0	38.0
60-61	25.2978068516978	26.0	11.0	37.0	11.0	38.0
62-63	25.138349844967102	26.0	11.0	37.0	11.0	38.0
64-65	25.093012175754367	26.0	11.0	37.0	11.0	38.0
66-67	25.36090902215836	26.0	11.0	37.0	11.0	38.0
68-69	25.070233683732887	25.0	11.0	37.0	11.0	38.0
70-71	25.118331694774255	25.0	11.0	37.0	11.0	38.0
72-73	24.953762383725326	25.0	11.0	37.0	11.0	38.0
74-75	25.05787642743704	25.0	11.0	37.0	11.0	38.0
76	24.540210239733796	25.0	11.0	36.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	60.0
13	568.0
14	1944.0
15	3471.0
16	4761.0
17	5593.0
18	5838.0
19	5131.0
20	3990.0
21	2937.0
22	2000.0
23	1416.0
24	1052.0
25	863.0
26	812.0
27	770.0
28	754.0
29	806.0
30	973.0
31	1103.0
32	1307.0
33	1547.0
34	2056.0
35	2898.0
36	4882.0
37	8581.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.32574038781499	20.913876032307833	15.924010043258614	36.83637353661857
2	14.007623196297306	23.41864052999365	37.619868711619326	24.953867562089723
3	12.53724570823565	22.232473720033276	34.686531044392346	30.543749527338726
4	10.449973530968766	38.637223020494595	22.41700068063223	28.49580276790441
5	10.654163200484005	40.313090826590035	28.87846933373667	20.15427663918929
6	23.42655756054029	42.208037753543174	13.665996097590488	20.699408588326047
7	17.89290576312207	29.86235062774164	21.107245499924367	31.13749810921192
8	14.838231512713081	47.82115468969794	14.24681983876091	23.09379395882807
9	18.22293485002042	23.117881075193996	15.897986658397494	42.761197416388086
10-11	16.234959577090912	34.6139593275201	21.751987113070705	27.399093982318284
12-13	15.663616425924525	26.017545186417607	25.831505709748164	32.487332677909706
14-15	25.551320446501496	28.843361466557766	18.86816710530296	26.737150981637775
16-17	26.87307625407437	26.40116767123702	18.008908787028563	28.71684728766005
18-19	18.385328039326904	26.066175080355453	23.246738513896766	32.30175836642088
20-21	25.457157982303563	25.22271799137866	19.628677304696364	29.691446721621418
22-23	26.286811973439416	24.458881006761153	18.81702539591306	30.437281623886374
24-25	18.229119399824544	32.57630758992044	17.70578092385879	31.488792086396224
26-27	23.627013537018833	32.37994403690539	19.34356802541027	24.649474400665508
28-29	18.830309401541335	30.913683701020243	23.179779614741765	27.076227282696657
30-31	20.594418815699918	28.76049308023898	23.167964909627166	27.47712319443394
32-33	17.371247069500114	31.11245556984043	22.684716025107768	28.831581335551693
34-35	16.72452259406315	33.56173189638873	22.10550198525241	27.608243524295705
36-37	22.91884123742531	26.569850994629757	22.879509870660314	27.63179789728462
38-39	23.354258054757224	24.343518378460143	25.633035849342008	26.66918771744063
40-41	19.261892157604173	30.209483475761928	22.869999243741965	27.658625122891934
42-43	14.684373322039795	29.538149725098123	25.16618896006171	30.611287992800367
44-45	15.024919267623101	25.045565580403395	28.774002283951084	31.155512868022416
46-47	17.940980730253802	27.575097558762142	24.933448286293373	29.550473424690686
48-49	19.084171519322393	28.65461695530515	23.926491718974514	28.334719806397946
50-51	25.605015579151164	27.988020691532807	19.522340200260157	26.884623529055872
52-53	13.503456412893858	31.79067903008668	23.472598284651106	31.23326627236836
54-55	16.682422876504088	27.477821559032844	22.64053908926586	33.199216475197204
56-57	18.498377667017103	29.33813352292065	20.54123146040222	31.62225734966003
58-59	16.692885991317368	30.200124037574312	22.05675475351314	31.050235217595183
60-61	14.680453544927119	27.677889306596676	22.493438121676512	35.148219026799694
62-63	16.934526285537093	28.823711815823504	21.88079049470205	32.36097140393735
64-65	17.133478053687725	27.718234008274777	21.61577502288044	33.532512915157064
66-67	14.855798685434646	35.42594791658788	18.0885100332045	31.629743364772978
68-69	15.84209332224155	37.183695076760195	17.005974438478408	29.968237162519852
70-71	14.48309763291235	35.84360583831203	18.236406261816533	31.43689026695909
72-73	14.018635043562439	35.12524201355276	20.057629477250728	30.798493465634074
74-75	14.156601447576406	32.9455986567943	19.787325765196147	33.11047413043314
76	12.509075508228463	32.05772023233301	18.492860600193612	36.94034365924492
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	0.0
8	0.0
9	0.0
10	1.5
11	1.5
12	0.0
13	1.0
14	2.0
15	9.0
16	13.5
17	11.0
18	22.0
19	36.5
20	66.0
21	92.0
22	98.5
23	143.5
24	200.0
25	218.0
26	269.0
27	330.5
28	427.5
29	514.0
30	565.0
31	715.5
32	929.5
33	1044.0
34	1149.5
35	1382.5
36	1615.0
37	1720.0
38	1919.0
39	2230.0
40	2531.0
41	2870.5
42	3021.0
43	3164.0
44	3412.0
45	3609.0
46	3701.0
47	3760.5
48	3750.0
49	3643.0
50	3606.0
51	3460.5
52	3267.5
53	3061.0
54	2902.0
55	2775.5
56	2488.5
57	2122.0
58	1916.0
59	1804.0
60	1490.0
61	1240.0
62	1192.0
63	1069.5
64	836.0
65	671.5
66	551.5
67	485.0
68	457.0
69	369.5
70	291.5
71	273.0
72	253.0
73	211.0
74	168.5
75	148.0
76	129.0
77	107.0
78	85.0
79	66.0
80	59.5
81	51.5
82	41.5
83	33.0
84	41.0
85	40.5
86	24.5
87	17.0
88	17.5
89	15.0
90	9.5
91	6.0
92	5.0
93	5.5
94	6.0
95	4.5
96	3.0
97	5.0
98	4.0
99	3.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001512516070483249
2	0.001512516070483249
3	0.0
4	0.0
5	0.0
6	0.003025032140966498
7	0.007562580352416245
8	0.003025032140966498
9	0.009075096422899493
10-11	0.002268774105724873
12-13	0.0
14-15	0.001512516070483249
16-17	7.562580352416245E-4
18-19	0.0037812901762081224
20-21	0.0
22-23	0.003025032140966498
24-25	0.001512516070483249
26-27	0.0
28-29	0.005293806246691371
30-31	0.0
32-33	0.0
34-35	0.0037812901762081224
36-37	0.01512516070483249
38-39	0.007562580352416245
40-41	0.0
42-43	7.562580352416245E-4
44-45	0.002268774105724873
46-47	0.001512516070483249
48-49	0.0
50-51	0.001512516070483249
52-53	0.009075096422899493
54-55	0.005293806246691371
56-57	0.009831354458141118
58-59	0.009075096422899493
60-61	0.02041896695152386
62-63	0.006806322317174619
64-65	0.01588141874007411
66-67	0.014368902669590865
68-69	0.0
70-71	0.0
72-73	0.004537548211449746
74-75	0.006806322317174619
76	0.004537548211449746
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	66115.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.15548665204568
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.96547937684441	81.95417076306435
2	3.529270468739277	6.222491113968085
3	1.871868780454327	4.950465098691673
4	0.9059089973234506	3.1944339408606215
5	0.4323656578134651	1.9057702488088937
6	0.17500514721021207	0.9256598351357482
7	0.0772081531809759	0.4764425622022234
8	0.022304577585615264	0.15730167133025788
9	0.006862946949420081	0.05445057853739695
>10	0.013725893898840161	0.15881418740074113
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0015125160704832488	0.0
6	0.0	0.0	0.0	0.0015125160704832488	0.0
7	0.0	0.0	0.0	0.0015125160704832488	0.0
8	0.0	0.0	0.0	0.0015125160704832488	0.0
9	0.0	0.0	0.0	0.006050064281932995	0.0
10	0.0	0.0	0.0	0.009075096422899493	0.0
11	0.0	0.0	0.0	0.009075096422899493	0.0
12	0.0	0.0	0.0	0.009075096422899493	0.0
13	0.0	0.0	0.0	0.009075096422899493	0.0
14	0.0	0.0	0.0	0.009075096422899493	0.0
15	0.0	0.0	0.0	0.009075096422899493	0.0
16	0.0	0.0	0.0	0.009075096422899493	0.0
17	0.0	0.0	0.0	0.01361264463434924	0.0
18	0.0	0.0	0.0	0.01361264463434924	0.0
19	0.0	0.0	0.0	0.01512516070483249	0.0
20	0.0	0.0	0.0	0.018150192845798985	0.0
21	0.0	0.0	0.0	0.02722528926869848	0.0
22	0.0	0.0	0.0	0.03932541783256447	0.0
23	0.0	0.0	0.0	0.04537548211449747	0.0
24	0.0	0.0	0.0	0.05445057853739696	0.0
25	0.0	0.0	0.0	0.06050064281932996	0.0
26	0.0	0.0	0.0	0.06050064281932996	0.0
27	0.0	0.0	0.0	0.08621341601754519	0.0
28	0.0	0.0	0.0	0.11646373742721017	0.0
29	0.0	0.0	0.0	0.15578915525977463	0.0
30	0.0	0.0	0.0	0.18755199273992287	0.0
31	0.0	0.0	0.0	0.2193148302200711	0.0
32	0.0	0.0	0.0	0.25712773198215233	0.0
33	0.0	0.0	0.0	0.3312410194358315	0.0
34	0.0	0.0	0.0	0.3872041140437117	0.0
35	0.0	0.0	0.0	0.4567798532859412	0.0
36	0.0	0.0	0.0	0.6428193299553808	0.0
37	0.0	0.0015125160704832488	0.0	0.9044846101489828	0.0
38	0.0	0.0015125160704832488	0.0	1.210012856386599	0.0
39	0.0	0.0015125160704832488	0.0	1.5578915525977464	0.0
40	0.0	0.0015125160704832488	0.0	1.9435831505709749	0.0
41	0.0	0.0015125160704832488	0.0	2.321712168191787	0.0
42	0.0	0.0015125160704832488	0.0	2.754291764349996	0.0
43	0.0	0.0015125160704832488	0.0	3.1263707176888755	0.0
44	0.0	0.0015125160704832488	0.0	3.5453376692127354	0.0
45	0.0	0.0015125160704832488	0.0	3.9416168796793465	0.0
46	0.0	0.0015125160704832488	0.0	4.324283445511608	0.0
47	0.0	0.0015125160704832488	0.0	4.669137109581789	0.0
48	0.0	0.0015125160704832488	0.0	5.02306587007487	0.0
49	0.0	0.0015125160704832488	0.0	5.314981471678137	0.0
50	0.0	0.0015125160704832488	0.0	5.632609846479619	0.0
51	0.0	0.0015125160704832488	0.0	5.953263253422068	0.0
52	0.0	0.0015125160704832488	0.0	6.276941692505483	0.0
53	0.0	0.0015125160704832488	0.0	6.541632004840052	0.0
54	0.0	0.0015125160704832488	0.0	6.883460636769266	0.0
55	0.0	0.0015125160704832488	0.0	7.184451334795432	0.0
56	0.0	0.0015125160704832488	0.0	7.471829388187249	0.0
57	0.0	0.0015125160704832488	0.0	7.671481509491039	0.0
58	0.0	0.0015125160704832488	0.0	7.889283823640626	0.0
59	0.0	0.0015125160704832488	0.0	8.11918626635408	0.0
60	0.0	0.0015125160704832488	0.0	8.36572638584285	0.0
61	0.0	0.0015125160704832488	0.0	8.612266505331618	0.0
62	0.0	0.0015125160704832488	0.0	8.777130757014293	0.0
63	0.0	0.0015125160704832488	0.0	9.002495651516297	0.0
64	0.0	0.0015125160704832488	0.0	9.174922483551388	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGTG	30	1.0055555E-7	70.038605	32
CTTACGT	25	2.802246E-6	70.038605	31
CCTTACG	30	1.0108488E-7	69.98563	30
TCGGGGG	15	0.0022174192	69.98563	45
CATCTCG	15	0.0022174192	69.98563	41
TGGCCGC	20	7.881563E-5	69.98563	49
GTTACTC	15	0.0022174192	69.98563	26
TCTCGCT	20	7.881563E-5	69.98563	43
ACGAGGG	15	0.0022174192	69.98563	24
AGTGTAG	65	0.0	64.651024	35
TTAGATC	40	1.0482836E-8	61.237423	38
TAGTGTA	35	2.9217517E-7	60.033092	34
TGATACA	35	2.9371222E-7	59.987682	2
TACGTGT	30	8.266283E-6	58.3655	33
ATCTCGC	30	8.30351E-6	58.321354	42
GGCGGTC	30	8.30351E-6	58.321354	47
CAGTGTA	25	2.368058E-4	56.030884	34
GCGGTCG	25	2.3769416E-4	55.988503	48
CGGTGGC	25	2.3769416E-4	55.988503	46
CGGTCGC	25	2.3769416E-4	55.988503	49
>>END_MODULE