FastQCFastQC Report
Wed 25 May 2016
SRR1779816_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779816_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66115
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2632539.81698555547153No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG33725.100204189669515No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC19512.950918853512819No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT6951.051198668985858TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC6631.002798154730394No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT3180.4809801104136731No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC3040.4598048854269077TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT2840.4295545640172427No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT1940.29342811767375027No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1760.26620282840505183No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC1710.2586402480526355RNA PCR Primer, Index 12 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1440.2178023141495878No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAGCTCGTATGCCGT1350.2041896695152386No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG1140.17242683203509038No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC1140.17242683203509038No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT1010.15276412311880813No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC970.14671405883687513TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCG960.14520154276639188No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT870.13158889813204264RNA PCR Primer, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT870.13158889813204264No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAATCTCGTATGCCGTC740.11192618921576042Illumina PCR Primer Index 8 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTCT207.858344E-570.0272546
ACTCTCG150.002218845369.9742836
CATCTCG150.002218845369.9742836
TCTACAC150.002218845369.974283
GGGAATT150.002218845369.9742825
GTATGCA207.887895E-569.9742842
CCCTTGA150.002218845369.9742840
CTACACA150.002218845369.974284
TGCAGTC207.887895E-569.9742845
TGCTTGC550.063.70936655
GCTTGCA550.063.70936656
TGCAAAA601.8189894E-1258.3560459
AAACCTA252.369956E-456.021869
TTGAACT252.3788467E-455.9794232
ATGCAGT252.3788467E-455.9794244
TATCGTA452.6595444E-854.4244438
TTGCAAA653.6379788E-1253.86711558
TATTTCA200.006891865352.56022652
GCTTGAC608.185452E-1152.56022656
CTTCTAC200.006912558352.520441
Produced by FastQC (version 0.11.5)