##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779816_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	66115
Sequences flagged as poor quality	0
Sequence length	76
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.483611888376316	34.0	34.0	34.0	34.0	34.0
2	33.583861453527945	34.0	34.0	34.0	34.0	34.0
3	33.72500945322544	34.0	34.0	34.0	34.0	34.0
4	33.52435907131513	34.0	34.0	34.0	34.0	34.0
5	33.58411858125993	34.0	34.0	34.0	34.0	34.0
6	37.302639340542996	38.0	38.0	38.0	37.0	38.0
7	37.30760039325418	38.0	38.0	38.0	37.0	38.0
8	37.228798305982004	38.0	38.0	38.0	36.0	38.0
9	37.13295016259548	38.0	38.0	38.0	36.0	38.0
10-11	37.08268169099297	38.0	37.5	38.0	35.5	38.0
12-13	37.244520910534675	38.0	38.0	38.0	36.5	38.0
14-15	37.25876124933828	38.0	38.0	38.0	36.0	38.0
16-17	37.04490660213265	38.0	38.0	38.0	35.0	38.0
18-19	36.83462149285336	38.0	37.5	38.0	34.5	38.0
20-21	36.93015957044544	38.0	37.5	38.0	35.0	38.0
22-23	37.03887166301142	38.0	37.5	38.0	35.5	38.0
24-25	37.1461544278908	38.0	38.0	38.0	36.0	38.0
26-27	37.06171065567572	38.0	38.0	38.0	35.5	38.0
28-29	36.3333585419345	38.0	37.0	38.0	34.0	38.0
30-31	36.436262572789836	38.0	37.0	38.0	34.0	38.0
32-33	36.512137941465625	38.0	37.0	38.0	34.0	38.0
34-35	36.376382061559404	38.0	37.0	38.0	34.0	38.0
36-37	36.37130757014293	38.0	37.0	38.0	33.5	38.0
38-39	34.55029872192392	37.0	35.0	38.0	25.5	38.0
40-41	36.06699689934206	38.0	36.5	38.0	32.5	38.0
42-43	36.96766240641307	38.0	37.0	38.0	35.5	38.0
44-45	36.72812523633064	38.0	37.0	38.0	35.0	38.0
46-47	36.89485744536036	38.0	37.5	38.0	35.5	38.0
48-49	36.39882023746502	38.0	37.0	38.0	34.0	38.0
50-51	36.44067155713529	38.0	37.0	38.0	33.5	38.0
52-53	36.3657188232625	38.0	37.0	38.0	34.0	38.0
54-55	36.42186341979884	38.0	37.0	38.0	34.0	38.0
56-57	36.26075020797096	38.0	37.0	38.0	34.0	38.0
58-59	36.25139529607502	38.0	37.0	38.0	34.0	38.0
60-61	35.739574982984195	38.0	37.0	38.0	31.0	38.0
62-63	35.69668002722529	38.0	37.0	38.0	31.5	38.0
64-65	36.27037737275958	38.0	37.0	38.0	34.0	38.0
66-67	35.78859562882856	38.0	36.5	38.0	32.0	38.0
68-69	35.592883611888375	38.0	36.5	38.0	31.0	38.0
70-71	32.33459124253195	36.0	29.5	38.0	18.5	38.0
72-73	26.579966724646447	28.0	16.0	38.0	10.0	38.0
74-75	25.615170536186945	25.0	11.0	38.0	11.0	38.0
76	25.761975345988052	25.0	11.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	3.0
23	6.0
24	23.0
25	50.0
26	111.0
27	201.0
28	432.0
29	712.0
30	1092.0
31	1735.0
32	2704.0
33	4404.0
34	7433.0
35	15818.0
36	14489.0
37	16902.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.96505591960894	9.868789442619974	12.370416332460614	60.79573830531048
2	8.243212584133706	9.577251758299932	64.87181426302654	17.307721394539815
3	8.211449746653559	12.204492172729335	65.93057551236483	13.653482568252286
4	6.198290856840354	53.06208878469334	27.205626559782196	13.53399379868411
5	6.4342433638357415	23.00839446419118	63.39257354609392	7.16478862587915
6	12.026015276412313	64.86878923088558	14.70165620509718	8.40353928760493
7	10.250321409664979	21.44294033124102	14.868032972850337	53.43870528624367
8	9.723965817136806	64.34697118656885	10.474173788096499	15.454889208197836
9	9.197610224608637	15.787642743704152	9.847992134916433	65.16675489677077
10-11	10.115707479391968	39.18021628979808	34.56552975875369	16.138546472056266
12-13	10.155032897224533	12.294486878923088	40.6995386825985	36.850941541253874
14-15	10.34409740603494	10.843227709294412	16.044770475686303	62.76790440898434
16-17	35.739242229448685	34.538304469484984	10.843983967329653	18.87846933373667
18-19	37.64425622022234	17.614762156847917	11.046661120774408	33.694320502155335
20-21	12.479013839522045	11.700068063223172	10.772139453981698	65.04877864327308
22-23	10.419723209559102	37.832564470997504	10.705588746880435	41.04212357256296
24-25	35.823457789138544	37.325397605668954	10.698863335576917	16.15228126961559
26-27	37.42721016410799	40.6450881040611	11.816531800650381	10.11116993118052
28-29	11.262194660818272	18.016335173561217	34.80677607199576	35.914694093624746
30-31	9.798079104590487	36.934129925130456	18.448158511684188	34.819632458594874
32-33	9.591620660969523	15.189442637828027	60.084700899947066	15.134235801255388
34-35	34.819632458594874	35.47001436890267	20.33502230961204	9.375330862890419
36-37	11.535960069575738	65.48060198139605	12.757316796490963	10.226121152537246
38-39	9.375330862890419	19.53414505029116	37.641987446116616	33.44853664070181
40-41	9.540951372608333	11.24253195190199	40.719957649550025	38.49655902593965
42-43	35.188686379792784	10.962716478862589	38.377070256371475	15.471526884973152
44-45	11.585873099901686	36.25198517734251	41.13816834303864	11.02397337971716
46-47	34.892989488013306	12.9615064660062	18.874688043560464	33.27081600242003
48-49	33.57634424865764	10.490055206836573	13.931029267185963	42.002571277319824
50-51	13.576269135354268	11.124220971743208	39.3386579536516	35.96085193925092
52-53	8.573697946215818	10.59646733991452	64.61136956768411	16.218465146185558
54-55	8.420335905583295	10.92298380995612	43.866696928430926	36.78998335602965
56-57	34.36814309277727	10.519346881951485	19.355204818183193	35.75730520708806
58-59	10.847019131118895	12.103515314729222	60.83529385065775	16.214171703494134
60-61	33.7032498184459	35.66630355846042	19.57304526748971	11.05740135560397
62-63	10.243415379495016	67.24936082661381	11.459735858761592	11.047487935129574
64-65	7.984327844549161	70.25240339159966	10.805618376963746	10.957650386887428
66-67	7.922447312283462	69.85188435178601	10.740275655476044	11.485392680454485
68-69	8.013916200272273	69.90772954167296	10.636061110270761	11.442293147783996
70-71	8.710121469738455	65.54449604429183	11.16750117234181	14.577881313627906
72-73	10.47448319680501	53.54179430741186	13.743599053000219	22.24012344278291
74-75	11.617758281651792	48.58871577673574	15.422780214793526	24.37074572681894
76	11.180528242519589	51.00444740265634	14.576589114452545	23.238435240371523
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.5
11	2.0
12	1.0
13	1.0
14	10.0
15	21.5
16	35.5
17	47.0
18	47.5
19	50.5
20	65.0
21	77.0
22	95.5
23	142.5
24	205.5
25	240.0
26	270.5
27	331.5
28	388.5
29	415.0
30	471.5
31	619.0
32	716.5
33	723.0
34	796.0
35	954.0
36	1252.5
37	1466.0
38	5430.0
39	11349.5
40	12075.5
41	8802.0
42	6758.0
43	5233.0
44	2912.0
45	1741.0
46	1366.0
47	1323.0
48	1152.0
49	955.5
50	887.0
51	899.5
52	876.0
53	804.5
54	769.0
55	749.5
56	715.0
57	632.0
58	564.0
59	554.0
60	522.5
61	431.0
62	361.0
63	342.0
64	328.0
65	277.5
66	226.0
67	230.0
68	203.0
69	178.5
70	155.0
71	129.0
72	143.5
73	136.5
74	98.0
75	81.0
76	75.0
77	54.5
78	46.0
79	52.0
80	51.5
81	39.0
82	27.5
83	28.0
84	25.5
85	18.0
86	13.0
87	13.0
88	17.0
89	12.5
90	4.5
91	3.5
92	2.0
93	2.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05747561067836346
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	7.562580352416245E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.010587612493382742
52-53	0.02646903123345686
54-55	0.03781290176208122
56-57	0.04840051425546397
58-59	0.027981547303940105
60-61	0.02873780533918173
62-63	0.021175224986765485
64-65	0.01588141874007411
66-67	0.027225289268698476
68-69	0.007562580352416245
70-71	0.012100128563865992
72-73	0.017393934810557364
74-75	0.007562580352416245
76	0.013612644634349238
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	66115.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.356953792634048
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.053050003714986	11.003554412765636
2	16.160190207296232	6.579444906602133
3	10.825469945761201	6.611207744082281
4	7.905490749684225	6.437268395976707
5	4.7626123783342	4.847614005898813
6	2.6896500482948213	3.2851849050896167
7	1.4488446392748346	2.0645844362096346
8	0.7207073333828665	1.173712470695001
9	0.4160784605096961	0.7623080995235575
>10	0.8172969760011887	3.0114194963321483
>50	0.08172969760011889	1.1933751796112833
>100	0.08172969760011889	3.1082205248430763
>500	0.014859945018203433	2.0539968237162523
>1k	0.014859945018203433	8.051123043182335
>5k	0.0	0.0
>10k+	0.0074299725091017165	39.81698555547153
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT	26325	39.81698555547153	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	3372	5.100204189669515	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	1951	2.950918853512819	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT	695	1.051198668985858	TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	663	1.002798154730394	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCGT	318	0.4809801104136731	No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	304	0.4598048854269077	TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAACTCGTATGCCGT	284	0.4295545640172427	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT	194	0.29342811767375027	No Hit
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	176	0.26620282840505183	No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	171	0.2586402480526355	RNA PCR Primer, Index 12 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	144	0.2178023141495878	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAAGCTCGTATGCCGT	135	0.2041896695152386	No Hit
CTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG	114	0.17242683203509038	No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC	114	0.17242683203509038	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT	101	0.15276412311880813	No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC	97	0.14671405883687513	TruSeq Adapter, Index 12 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAACCTCGTATGCCG	96	0.14520154276639188	No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT	87	0.13158889813204264	RNA PCR Primer, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT	87	0.13158889813204264	No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAATCTCGTATGCCGTC	74	0.11192618921576042	Illumina PCR Primer Index 8 (95% over 22bp)
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0030250321409664976	0.0
2	0.0	0.0	0.0	0.0030250321409664976	0.0
3	0.0	0.0	0.0	0.0030250321409664976	0.0
4	0.0	0.0	0.0	0.0030250321409664976	0.0
5	0.0	0.0	0.0	0.01210012856386599	0.0
6	0.0	0.0	0.0	0.01210012856386599	0.0
7	0.0	0.0	0.0	0.01210012856386599	0.0
8	0.0	0.0	0.0	0.01210012856386599	0.0
9	0.0	0.0	0.0	0.019662708916282235	0.0
10	0.0	0.0	0.0	0.022687741057248734	0.0
11	0.0	0.0	0.0	0.022687741057248734	0.0
12	0.0	0.0	0.0	0.022687741057248734	0.0
13	0.0	0.0	0.0	0.022687741057248734	0.0
14	0.0	0.0	0.0	0.022687741057248734	0.0
15	0.0	0.0	0.0	0.022687741057248734	0.0
16	0.0	0.0	0.0	0.022687741057248734	0.0
17	0.0	0.0	0.0	0.02722528926869848	0.0
18	0.0	0.0	0.0	0.02873780533918173	0.0
19	0.0	0.0	0.0	0.03327535355063148	0.0
20	0.0	0.0	0.0	0.03630038569159797	0.0
21	0.0	0.0	0.0	0.03781290176208122	0.0
22	0.0	0.0	0.0	0.03781290176208122	0.0
23	0.0	0.0	0.0	0.03932541783256447	0.0
24	0.0	0.0	0.0	0.04083793390304772	0.0
25	0.0	0.0	0.0	0.04991303032594721	0.0
26	0.0	0.0	0.0	0.05293806246691371	0.0
27	0.0	0.0	0.0	0.08016335173561219	0.0
28	0.0	0.0	0.0	0.11192618921576042	0.0
29	0.0	0.0	0.0	0.15730167133025788	0.0
30	0.0	0.0	0.0	0.19360205702185584	0.0
31	0.0	0.0	0.0	0.22687741057248734	0.0
32	0.0	0.0	0.0	0.2646903123345686	0.0
33	0.0	0.0	0.0	0.3433411479996975	0.0
34	0.0	0.0	0.0	0.40989185510096043	0.0
35	0.0	0.0	0.0	0.4824926264841564	0.0
36	0.0	0.0	0.0	0.6836572638584285	0.0
37	0.0	0.0	0.0	0.978597897602662	0.0
38	0.0	0.0	0.0	1.2916887241926946	0.0
39	0.0	0.0	0.0	1.6501550328972245	0.0
40	0.0	0.0	0.0	2.0645844362096346	0.0
41	0.0	0.0	0.0	2.4699387430991453	0.0
42	0.0	0.0	0.0	2.925206080314603	0.0
43	0.0	0.0	0.0	3.3048476140058987	0.0
44	0.0	0.0	0.0	3.732889661952658	0.0
45	0.0	0.0	0.0	4.151856613476518	0.0
46	0.0	0.0	0.0	4.5753611132118275	0.0
47	0.0	0.0	0.0	4.942902518339257	0.0
48	0.0	0.0	0.0	5.31649398774862	0.0
49	0.0	0.0	0.0	5.620509717915753	0.0
50	0.0	0.0	0.0	5.971413446267866	0.0
51	0.0	0.0	0.0	6.3041669817741814	0.0
52	0.0	0.0	0.0	6.645995613703396	0.0
53	0.0	0.0	0.0	6.9106859260379645	0.0
54	0.0	0.0	0.0	7.257052106178628	0.0
55	0.0	0.0	0.0	7.568630416698177	0.0
56	0.0	0.0	0.0	7.871133630794827	0.0
57	0.0	0.0	0.0	8.078348332451032	0.0
58	0.0	0.0	0.0	8.303713226953036	0.0
59	0.0	0.0	0.0	8.548740830371322	0.0
60	0.0	0.0	0.0	8.79830598200106	0.0
61	0.0	0.0	0.0	9.05089616577176	0.0
62	0.0	0.0	0.0	9.211222869242986	0.0
63	0.0	0.0	0.0	9.447175376238372	0.0
64	0.0	0.0	0.0	9.634727368978295	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAGTCT	20	7.858344E-5	70.02725	46
ACTCTCG	15	0.0022188453	69.97428	36
CATCTCG	15	0.0022188453	69.97428	36
TCTACAC	15	0.0022188453	69.97428	3
GGGAATT	15	0.0022188453	69.97428	25
GTATGCA	20	7.887895E-5	69.97428	42
CCCTTGA	15	0.0022188453	69.97428	40
CTACACA	15	0.0022188453	69.97428	4
TGCAGTC	20	7.887895E-5	69.97428	45
TGCTTGC	55	0.0	63.709366	55
GCTTGCA	55	0.0	63.709366	56
TGCAAAA	60	1.8189894E-12	58.35604	59
AAACCTA	25	2.369956E-4	56.0218	69
TTGAACT	25	2.3788467E-4	55.97942	32
ATGCAGT	25	2.3788467E-4	55.97942	44
TATCGTA	45	2.6595444E-8	54.42444	38
TTGCAAA	65	3.6379788E-12	53.867115	58
TATTTCA	20	0.0068918653	52.560226	52
GCTTGAC	60	8.185452E-11	52.560226	56
CTTCTAC	20	0.0069125583	52.52044	1
>>END_MODULE