FastQCFastQC Report
Wed 25 May 2016
SRR1779815_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779815_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183392
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATGC252.8292434E-670.0092826
CGATACT352.9593684E-760.00795425
ACCGGAT200.006946620552.49263454
GTTCTAT200.006946620552.4926341
CATCTCG200.006946620552.49263441
TACGGTC200.006946620552.49263410
TGTGCGT200.006946620552.4926348
CGCGTGT200.006946620552.4926346
CGAATAG506.156915E-849.00649625
CGGTGGT1250.047.60630846
GACGAAT604.8967195E-946.67284823
CGGTGTA305.8704696E-446.66012234
TAGATCA451.6796148E-646.66012239
TCGGTGG1700.045.28776645
TGGTCGC1100.044.55135749
CTTCCGA404.5834313E-543.74386218
TCGGCGG404.5834313E-543.74386245
GCCGTAT659.958967E-943.0708854
CTCGGTG1550.042.8972144
GTAGATC2650.042.25822438