##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779815_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	183392
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.863794494852556	33.0	23.0	34.0	12.0	34.0
2	28.649090472866863	34.0	23.0	34.0	12.0	34.0
3	28.125212659221777	34.0	23.0	34.0	11.0	34.0
4	28.696704327342523	34.0	23.0	34.0	12.0	34.0
5	29.237158654685047	34.0	27.0	34.0	11.0	34.0
6	31.298655339382307	37.0	27.0	38.0	12.0	38.0
7	31.615555749432907	37.0	27.0	38.0	12.0	38.0
8	30.880163802128774	37.0	26.0	38.0	11.0	38.0
9	32.00670149188623	37.0	28.0	38.0	12.0	38.0
10-11	30.529886799860407	36.5	25.0	38.0	11.0	38.0
12-13	31.296179767928805	37.0	27.0	38.0	11.0	38.0
14-15	30.139174555051476	35.5	24.5	38.0	11.0	38.0
16-17	30.705011668993194	36.5	25.0	38.0	11.0	38.0
18-19	31.55465887279707	37.0	27.0	38.0	11.0	38.0
20-21	30.30451709998255	36.0	24.5	38.0	11.0	38.0
22-23	31.17090440150061	37.0	26.0	38.0	11.0	38.0
24-25	31.48845642121794	37.0	27.0	38.0	11.0	38.0
26-27	30.393427739487002	37.0	24.5	38.0	11.0	38.0
28-29	31.548028267318095	37.0	27.0	38.0	11.0	38.0
30-31	31.993843788169606	37.0	31.0	38.0	11.0	38.0
32-33	32.11543578782063	37.5	31.0	38.0	11.0	38.0
34-35	32.02855086372361	37.0	29.0	38.0	11.0	38.0
36-37	31.50848183126854	37.0	26.5	38.0	11.0	38.0
38-39	31.418690564473913	37.0	26.5	38.0	11.0	38.0
40-41	31.38484775780841	37.0	26.5	38.0	11.0	38.0
42-43	31.85268714011516	37.0	29.0	38.0	11.0	38.0
44-45	31.87406211830396	37.0	27.0	38.0	11.0	38.0
46-47	31.7700908436573	37.0	27.0	38.0	11.0	38.0
48-49	31.438323372884312	37.0	26.5	38.0	11.0	38.0
50-51	30.782566851334845	37.0	25.5	38.0	11.0	38.0
52-53	31.37562979846449	37.0	26.5	38.0	11.0	38.0
54-55	31.532266947304137	37.0	27.0	38.0	11.0	38.0
56-57	31.599186442156693	37.0	27.0	38.0	11.0	38.0
58-59	31.856490468504624	37.0	28.5	38.0	11.0	38.0
60-61	31.735301976094924	37.0	27.0	38.0	11.0	38.0
62-63	31.73727588989705	37.0	27.0	38.0	11.0	38.0
64-65	31.73681240184959	37.0	28.5	38.0	11.0	38.0
66-67	31.654764111847847	37.0	27.0	38.0	11.0	38.0
68-69	31.011960718024778	37.0	25.5	38.0	11.0	38.0
70-71	31.031498102425406	37.0	25.5	38.0	11.0	38.0
72-73	31.220732093002965	37.0	26.5	38.0	11.0	38.0
74-75	31.32468428284767	37.0	27.0	38.0	11.0	38.0
76	30.30605478974001	36.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	50.0
13	514.0
14	1868.0
15	3413.0
16	4719.0
17	5536.0
18	5856.0
19	5230.0
20	4379.0
21	3372.0
22	2779.0
23	2462.0
24	2432.0
25	2381.0
26	2589.0
27	2888.0
28	3274.0
29	3907.0
30	4595.0
31	5667.0
32	6916.0
33	8736.0
34	11633.0
35	16630.0
36	26831.0
37	44735.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.45964927586808	18.75981504100506	9.666724829872622	34.113810853254236
2	19.530841039958123	24.143365032280578	30.43207991624498	25.893714011516316
3	16.910770371662885	21.048900715407434	30.630561856569532	31.409767056360145
4	12.848979235735476	22.991733554353516	31.649145000872448	32.51014220903856
5	13.050187576339209	32.757699354388414	35.533174838597105	18.658938230675275
6	25.757146128930238	38.200298823247145	18.91911051007165	17.123444537750974
7	28.156594575139877	28.123875274024147	19.43526486274253	24.284265288093447
8	22.535948567782885	36.81504141514939	19.531918838303696	21.117091178764035
9	22.937230735671047	18.001854174619623	19.403937394339312	39.65697769537002
10-11	22.46672973997257	27.096619398040726	24.63703311893862	25.799617743048074
12-13	22.174904030710174	22.855958820450184	26.311943814343046	28.6571933344966
14-15	24.79020235453212	25.202981607603427	22.226281551439275	27.78053448642518
16-17	25.555367736869655	26.119732594660615	22.480260861978714	25.844638806491016
18-19	21.50567244589978	25.888492337222207	25.546591488468344	27.059243728409673
20-21	24.351116733554353	25.092152329436402	24.281866166463097	26.274864770546152
22-23	25.652371877189683	24.706020279352302	23.351791415492247	26.289816427965768
24-25	21.545810476263185	28.781095612533125	22.942863686431895	26.730230224771795
26-27	23.34241227101583	28.47514743049705	24.124346002949974	24.058094295537146
28-29	21.840167957247246	27.91716653942633	25.469516850256298	24.77314865307013
30-31	22.62591232418078	26.651725952402373	25.370041686773924	25.352320036642915
32-33	21.088160879427676	28.214153289129296	25.019357441982205	25.678328389460823
34-35	21.129921012953655	28.727891397582674	25.113900029718977	25.02828755974469
36-37	23.885904393117176	25.871124321671076	24.990319325897847	25.252651959313898
38-39	23.733864854695074	25.516570051207104	26.440642842730387	24.30892225136743
40-41	21.893658903653943	27.265242023872492	25.72618067407888	25.114918398394686
42-43	20.128469732454693	26.8265086059998	26.595306710580484	26.44971495096502
44-45	19.775440816003794	25.202105992152973	27.870314072030165	27.152139119813068
46-47	20.70714231184496	25.732607723599404	27.10948491160721	26.450765052948427
48-49	20.951682470786462	26.068345774874444	27.17990522980953	25.80006652452956
50-51	23.615231094728117	25.777586319715574	25.269101576984315	25.33808100857199
52-53	18.63689039769861	27.38762321567344	26.82563704036975	27.149849346258197
54-55	19.520980288971476	26.058421427188623	26.478599805316446	27.941998478523463
56-57	19.977858430293594	26.867649585118247	25.81619770349384	27.338294281094317
58-59	19.256191135809605	27.139016289735885	26.087027654921553	27.51776491953296
60-61	18.412655990924165	26.05348416092155	26.47209616895017	29.061763679204116
62-63	19.46927991231012	26.552909771586407	25.99803134040633	27.979778975697144
64-65	19.935534363947237	26.18330165499977	25.455745322559974	28.42541865849302
66-67	19.00776639724249	28.811752129759917	23.93184768262487	28.248633790372722
68-69	19.257267960990347	29.75753924003697	23.222576960093352	27.762615838879327
70-71	19.309184697260513	28.721263741057406	23.641434740882914	28.328116820799167
72-73	19.141539862530777	28.34996032968621	24.330853143497478	28.17764666428554
74-75	18.794427766280297	27.62967386236288	24.09944730290909	29.47645106844773
76	18.322009313688067	26.925719520574088	23.60812711984557	31.14414404589227
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.5
14	1.5
15	4.0
16	13.5
17	21.0
18	34.5
19	52.0
20	83.0
21	110.0
22	130.0
23	190.5
24	266.0
25	301.0
26	418.0
27	602.0
28	840.5
29	1012.0
30	1190.0
31	1577.0
32	2194.0
33	2602.0
34	2896.5
35	3564.0
36	4370.5
37	4804.0
38	5385.0
39	6754.5
40	8176.5
41	9220.0
42	9630.0
43	9995.5
44	10751.0
45	11079.5
46	11018.0
47	11308.5
48	11370.0
49	10732.0
50	10323.0
51	9818.5
52	8670.5
53	7759.0
54	7491.0
55	6949.5
56	6034.5
57	5143.5
58	4626.0
59	4187.0
60	3405.0
61	2799.5
62	2537.0
63	2322.0
64	1974.0
65	1716.0
66	1464.0
67	1337.0
68	1239.5
69	1048.0
70	891.0
71	828.0
72	778.0
73	716.0
74	609.0
75	514.0
76	499.0
77	450.0
78	361.5
79	307.0
80	285.5
81	245.0
82	198.0
83	170.0
84	153.5
85	129.0
86	99.0
87	77.0
88	71.0
89	51.5
90	34.5
91	22.0
92	13.0
93	14.5
94	12.0
95	7.5
96	7.0
97	8.5
98	10.0
99	9.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0032716803350200663
7	0.007633920781713488
8	0.0027264002791833886
9	0.01199616122840691
10-11	0.004089600418775083
12-13	0.0
14-15	5.452800558366777E-4
16-17	0.0
18-19	0.0029990403071017274
20-21	0.0
22-23	0.001908480195428372
24-25	0.0032716803350200663
26-27	2.7264002791833886E-4
28-29	0.0065433606700401326
30-31	2.7264002791833886E-4
32-33	0.0
34-35	0.004089600418775083
36-37	0.020175362065957075
38-39	0.010360321060896877
40-41	5.452800558366777E-4
42-43	0.0013632001395916943
44-45	0.005725440586285116
46-47	0.0032716803350200663
48-49	5.452800558366777E-4
50-51	0.002181120223346711
52-53	0.013359361367998606
54-55	0.00954240097714186
56-57	0.014449921479671958
58-59	0.013632001395916943
60-61	0.02617344268016053
62-63	0.010087681032978538
64-65	0.020993282149712092
66-67	0.020720642121793753
68-69	8.179200837550166E-4
70-71	0.0
72-73	0.0029990403071017274
74-75	0.010087681032978538
76	0.0032716803350200663
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	183392.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.54817392906351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.33677776900826	51.210441304054044
2	8.539437908585432	11.024102467596219
3	4.921211645310821	9.529656756697669
4	2.8803085937813995	7.436746403231124
5	1.6799548171308138	5.421900786456393
6	0.8835596832982715	3.421929846854706
7	0.47697003894364165	2.155128152288046
8	0.3328690295738146	1.7188870413223332
9	0.21164246565082956	1.2295021215245037
>10	0.737268048716735	6.851705119974973
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002181120223346711	0.0
2	0.0	0.0	0.0	0.002181120223346711	0.0
3	0.0	0.0	0.0	0.002181120223346711	0.0
4	0.0	0.0	0.0	0.004362240446693422	0.0
5	0.0	0.0	0.0	0.004907520502530099	0.0
6	0.0	0.0	0.0	0.005452800558366777	0.0
7	0.0	0.0	0.0	0.005998080614203455	0.0
8	0.0	0.0	0.0	0.005998080614203455	0.0
9	0.0	0.0	0.0	0.005998080614203455	0.0
10	0.0	0.0	0.0	0.00708864072587681	0.0
11	0.0	0.0	0.0	0.007633920781713488	0.0
12	0.0	0.0	0.0	0.008179200837550166	0.0
13	0.0	0.0	0.0	0.008179200837550166	0.0
14	0.0	0.0	0.0	0.009815041005060199	0.0
15	0.0	0.0	0.0	0.009815041005060199	0.0
16	0.0	0.0	0.0	0.009815041005060199	0.0
17	0.0	0.0	0.0	0.009815041005060199	0.0
18	0.0	0.0	0.0	0.011450881172570232	0.0
19	0.0	0.0	0.0	0.015267841563426976	0.0
20	0.0	0.0	0.0	0.01635840167510033	0.0
21	0.0	0.0	0.0	0.024537602512650497	0.0
22	0.0	0.0	0.0	0.034352643517710696	0.0
23	0.0	0.0	0.0	0.034352643517710696	0.0
24	0.0	0.0	0.0	0.03980544407607747	0.0
25	0.0	0.0	0.0	0.04253184435526086	0.0
26	0.0	0.0	0.0	0.04525824463444425	0.0
27	0.0	0.0	0.0	0.06488832664456465	0.0
28	0.0	0.0	0.0	0.0856089687663584	0.0
29	0.0	0.0	0.0	0.10251265049729541	0.0
30	0.0	0.0	0.0	0.1346841737916594	0.0
31	0.0	0.0	0.0	0.17776129820275693	0.0
32	0.0	0.0	0.0	0.2153856220554877	0.0
33	0.0	0.0	0.0	0.26064386668993195	0.0
34	0.0	0.0	0.0	0.3162624323852731	0.0
35	0.0	0.0	0.0	0.4056883615424882	0.0
36	0.0	0.0	0.0	0.5730893386843483	0.0
37	0.0	0.0	0.0	0.8042880823590997	0.0
38	0.0	0.0	0.0	1.1041921130692725	0.0
39	0.0	0.0	0.0	1.4793447914849067	0.0
40	0.0	0.0	0.0	1.947740359448613	0.0
41	0.0	0.0	0.0	2.5137410574070844	0.0
42	0.0	0.0	0.0	3.089011516314779	0.0
43	0.0	0.0	0.0	3.7346231024254055	0.0
44	0.0	0.0	0.0	4.403681730937009	0.0
45	0.0	0.0	0.0	5.085281800732856	0.0
46	0.0	0.0	0.0	5.795781713488047	0.0
47	0.0	0.0	0.0	6.512279706857442	0.0
48	0.0	0.0	0.0	7.172068574419822	0.0
49	0.0	0.0	0.0	7.809500959692898	0.0
50	0.0	0.0	0.0	8.394041179549816	0.0
51	0.0	0.0	0.0	8.978581399406735	0.0
52	0.0	0.0	0.0	9.715254754842087	0.0
53	0.0	0.0	0.0	10.442658349328214	0.0
54	0.0	0.0	0.0	11.18860146571279	0.0
55	0.0	0.0	0.0	11.78132088640726	0.0
56	0.0	0.0	0.0	12.395851509335195	0.0
57	0.0	0.0	0.0	12.982027569359623	0.0
58	0.0	0.0	0.0	13.525126504972954	0.0
59	0.0	0.0	0.0	14.016969115337638	0.0
60	0.0	0.0	0.0	14.50390420519979	0.0
61	0.0	0.0	0.0	15.029008898970512	0.0
62	0.0	0.0	0.0	15.48540830570581	0.0
63	0.0	0.0	0.0	16.06176932472518	0.0
64	0.0	0.0	0.0	16.56179113592741	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATGC	25	2.8292434E-6	70.00928	26
CGATACT	35	2.9593684E-7	60.007954	25
ACCGGAT	20	0.0069466205	52.492634	54
GTTCTAT	20	0.0069466205	52.492634	1
CATCTCG	20	0.0069466205	52.492634	41
TACGGTC	20	0.0069466205	52.492634	10
TGTGCGT	20	0.0069466205	52.492634	8
CGCGTGT	20	0.0069466205	52.492634	6
CGAATAG	50	6.156915E-8	49.006496	25
CGGTGGT	125	0.0	47.606308	46
GACGAAT	60	4.8967195E-9	46.672848	23
CGGTGTA	30	5.8704696E-4	46.660122	34
TAGATCA	45	1.6796148E-6	46.660122	39
TCGGTGG	170	0.0	45.287766	45
TGGTCGC	110	0.0	44.551357	49
CTTCCGA	40	4.5834313E-5	43.743862	18
TCGGCGG	40	4.5834313E-5	43.743862	45
GCCGTAT	65	9.958967E-9	43.07088	54
CTCGGTG	155	0.0	42.89721	44
GTAGATC	265	0.0	42.258224	38
>>END_MODULE