SRR1779815_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1779815_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	183392
Sequences flagged as poor quality	0
Sequence length	76
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	24739	13.489683301343568	TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	3153	1.719268016053045	TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	1710	0.9324288954807188	RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT	696	0.3795149188623277	TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	669	0.3647923573547374	RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT	406	0.22138370266969112	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT	397	0.21647618216716102	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT	246	0.13413889373582272	No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	238	0.1297766532891293	TruSeq Adapter, Index 5 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCTCGTA	3435	53.884853	38
GATCTCG	3400	53.822086	36
ATCTCGT	3440	53.70482	37
CTTGAAA	3545	53.53974	57
CCGTCTT	3585	53.430763	47
ATGCCGT	3630	53.303566	44
CGTATGC	3685	53.267597	41
CTCGTAT	3620	53.2575	39
GTCTTCT	3620	53.20437	49
CGTCTTC	3575	53.188404	48
TGCCGTC	3635	53.162983	45
TCGTATG	3690	53.100597	40
GCCGTCT	3635	53.081154	46
TATGCCG	3655	53.034702	43
AGTGATC	3385	53.026924	33
CTTCTGC	3630	52.961338	51
TTGAAAA	3565	52.94469	58
TGAAAAA	3590	52.673542	59
GCTTGAA	3610	52.67273	56
CTGCTTG	3670	52.67035	54