##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779815_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	183392
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.508397312859884	34.0	34.0	34.0	34.0	34.0
2	33.60714753097191	34.0	34.0	34.0	34.0	34.0
3	33.72115468504624	34.0	34.0	34.0	34.0	34.0
4	33.511009204327344	34.0	34.0	34.0	34.0	34.0
5	33.577904161577386	34.0	34.0	34.0	34.0	34.0
6	37.22700008724481	38.0	38.0	38.0	36.0	38.0
7	37.28932559762694	38.0	38.0	38.0	36.0	38.0
8	37.181829087419295	38.0	38.0	38.0	36.0	38.0
9	37.076491886232766	38.0	38.0	38.0	36.0	38.0
10-11	37.03089829436398	38.0	37.5	38.0	35.5	38.0
12-13	37.216833340603735	38.0	38.0	38.0	36.0	38.0
14-15	37.23777754754842	38.0	38.0	38.0	36.0	38.0
16-17	37.128421632350374	38.0	38.0	38.0	36.0	38.0
18-19	36.98009182516141	38.0	37.5	38.0	35.5	38.0
20-21	37.002579174664106	38.0	37.5	38.0	35.5	38.0
22-23	36.991420018321406	38.0	37.5	38.0	35.5	38.0
24-25	37.158886429069966	38.0	38.0	38.0	36.0	38.0
26-27	37.05833133397313	38.0	38.0	38.0	36.0	38.0
28-29	36.557382546675974	38.0	37.0	38.0	34.0	38.0
30-31	36.78225058890246	38.0	37.0	38.0	34.5	38.0
32-33	36.80192429331704	38.0	37.0	38.0	35.0	38.0
34-35	36.87881696039085	38.0	37.5	38.0	35.5	38.0
36-37	36.92269564648403	38.0	37.5	38.0	35.5	38.0
38-39	36.030868303960915	38.0	37.0	38.0	33.0	38.0
40-41	36.59300023992323	38.0	37.0	38.0	34.5	38.0
42-43	37.07757971994417	38.0	37.5	38.0	36.0	38.0
44-45	36.84061191327866	38.0	37.5	38.0	35.0	38.0
46-47	36.99857136625371	38.0	38.0	38.0	36.0	38.0
48-49	36.59217414063863	38.0	37.0	38.0	34.0	38.0
50-51	36.761061005932646	38.0	37.0	38.0	34.5	38.0
52-53	36.6912188099808	38.0	37.0	38.0	34.0	38.0
54-55	36.70435188012563	38.0	37.0	38.0	34.5	38.0
56-57	36.571668884138894	38.0	37.0	38.0	34.0	38.0
58-59	36.64987840254754	38.0	37.0	38.0	34.0	38.0
60-61	36.29518190542663	38.0	37.0	38.0	34.0	38.0
62-63	36.1331328520328	38.0	37.0	38.0	34.0	38.0
64-65	36.54265180596755	38.0	37.0	38.0	34.0	38.0
66-67	36.26973368522073	38.0	37.0	38.0	33.0	38.0
68-69	36.331132219507936	38.0	37.0	38.0	34.0	38.0
70-71	35.2391162100855	38.0	36.5	38.0	28.5	38.0
72-73	33.31112589425929	38.0	34.0	38.0	20.0	38.0
74-75	32.996949158087595	38.0	34.0	38.0	11.0	38.0
76	32.77240010469377	38.0	33.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	3.0
22	8.0
23	17.0
24	49.0
25	125.0
26	220.0
27	377.0
28	768.0
29	1179.0
30	1944.0
31	3063.0
32	4744.0
33	7423.0
34	12750.0
35	24209.0
36	31671.0
37	94841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.311666348435665	16.162134148004693	11.418128256185046	38.10807124737459
2	16.993107660094225	16.466912406211833	43.40538300471122	23.134596928982727
3	15.884553306578258	18.385752922701098	41.51380649101378	24.21588727970686
4	11.449790612458559	28.87039783632874	33.3476923748037	26.332119176409
5	11.574659745245159	30.50187576339208	45.22007503053568	12.703389460827081
6	24.669560286162973	46.00200663060548	16.795716279881347	12.5327168033502
7	23.54737393125109	26.600942243936487	18.686747513522945	31.164936311289477
8	20.628489792357353	44.39452102599895	16.34204327342523	18.634945908218462
9	20.021047810155295	16.053044843831792	17.15341999651021	46.772487349502704
10-11	20.88831573896353	29.640878555225964	28.885393037864247	20.585412667946258
12-13	21.084616559064738	19.144510120397836	30.495332402722035	29.27554091781539
14-15	19.063263392078174	19.367529663235036	20.90930902111324	40.659897923573546
16-17	27.94614814168557	29.614977752573722	20.27814735648229	22.16072674925842
18-19	28.05602207293666	23.423595358576165	21.371433868434828	27.148948700052344
20-21	19.515573198394694	21.357801867038912	20.98537558890246	38.14124934566394
22-23	19.16495812249171	30.321660704938058	21.010458471470947	29.502922701099283
24-25	27.73398840179835	30.60245759731284	20.905935694597048	20.757618306291764
26-27	27.90825117780492	31.26990272203804	21.480489879602164	19.34135622055488
28-29	18.959660181469204	31.816818618042227	21.916986564299425	27.306534636189145
30-31	18.66793535159658	31.375687052870354	21.800023992322455	28.15635360321061
32-33	18.617769586459605	40.16832795323678	21.672428459256672	19.54147400104694
34-35	27.70268059675449	24.23469944163322	29.763839207817135	18.29878075379515
36-37	28.3450205025301	28.746891903681732	23.72649842959344	19.181589164194733
38-39	19.612905688361543	23.232202058977492	30.318116384575116	26.836775868085848
40-41	18.29087419298552	20.65248211481417	32.14262345140464	28.914020240795672
42-43	27.309261036468328	20.767536206595707	30.72135098586634	21.20185177106962
44-45	18.691927674053392	29.239825074158087	32.2966650671785	19.771582184610015
46-47	26.791244983423486	20.701012039783635	24.99209343919037	27.51564953760251
48-49	26.03685002617344	20.03386189146746	23.54873713139068	30.380550950968416
50-51	19.06643433872432	20.355145436746536	32.2161413382853	28.362278886243843
52-53	16.82466523031609	20.521722529795184	40.89671912073526	21.75689311915346
54-55	16.415483948401388	20.758921873763978	34.03828161015169	28.787312567682942
56-57	25.2776360764587	20.673692021048783	25.042760456875364	29.005911445617155
58-59	17.302830211836323	20.84761803458875	39.37940984751167	22.470141906063258
60-61	25.193843904860348	28.97035681460532	25.09729695822572	20.738502322308616
62-63	16.787984738195693	40.08596355821495	21.940889144646235	21.185162558943126
64-65	16.138831472684736	41.20768546948882	21.271385642373705	21.382097415452744
66-67	16.216356277070446	41.1792622655713	20.550355120610085	22.054026336748166
68-69	16.289297886843897	41.36987048398091	20.269393319700068	22.07143830947512
70-71	17.0318358442975	39.516258777012744	20.425659554161836	23.026245824527916
72-73	17.360156642014093	36.04075310338809	20.756157689203043	25.842932565394765
74-75	17.885686705987254	34.835259824011736	20.93834957148194	26.34070389851907
76	17.790648211773174	35.739450061625384	20.320561063665018	26.14934066293642
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	3.5
15	9.0
16	14.0
17	17.0
18	28.5
19	34.0
20	74.0
21	120.0
22	128.5
23	199.5
24	332.5
25	403.0
26	505.0
27	670.5
28	901.0
29	1068.0
30	1259.5
31	1538.0
32	2041.0
33	2457.0
34	2775.0
35	3402.5
36	3993.5
37	4275.0
38	4961.0
39	10268.5
40	17203.0
41	18264.5
42	17013.0
43	15145.5
44	12263.0
45	10583.5
46	9919.0
47	9743.0
48	9059.0
49	8299.0
50	8047.0
51	7481.0
52	6313.0
53	5304.0
54	4897.0
55	4616.0
56	4088.0
57	3452.5
58	3064.0
59	2706.5
60	2193.0
61	1844.5
62	1652.0
63	1562.5
64	1355.0
65	1199.5
66	1083.5
67	1005.0
68	954.0
69	864.0
70	740.0
71	655.0
72	643.0
73	592.0
74	513.0
75	473.0
76	431.0
77	380.0
78	336.5
79	302.0
80	277.0
81	241.0
82	179.5
83	129.0
84	110.5
85	76.5
86	60.5
87	60.0
88	42.5
89	17.0
90	10.5
91	10.5
92	9.0
93	9.0
94	5.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04743936485779096
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	8.179200837550166E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.01663104170301867
52-53	0.031080963182690626
54-55	0.05043840516489269
56-57	0.05643648577909614
58-59	0.03626112371313907
60-61	0.03353472343395568
62-63	0.031898883266445646
64-65	0.017994241842610365
66-67	0.04035072413191415
68-69	0.009269760949223521
70-71	0.016085761647181993
72-73	0.02399232245681382
74-75	0.014995201535508635
76	0.01417728145175362
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	183392.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.435678764613506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	42.482903509336076	10.805814866515442
2	14.637597273136535	7.446344442505672
3	11.876433639891097	9.062554528005585
4	9.308208459279268	9.470424009771419
5	6.851458829077968	8.713575292270109
6	4.4182905653096665	6.742933170476356
7	3.052715072780672	5.435351596580004
8	1.8457778635282012	3.755889024603036
9	1.2433811391943748	2.8463618914674575
>10	4.197483225073424	16.636494503577037
>50	0.04287521169635775	0.744307276217065
>100	0.03215640877226831	1.4542619089164195
>500	0.004287521169635775	0.744307276217065
>1k	0.004287521169635775	2.651696911533764
>5k	0.0	0.0
>10k+	0.0021437605848178875	13.489683301343568
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	24739	13.489683301343568	TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	3153	1.719268016053045	TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	1710	0.9324288954807188	RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT	696	0.3795149188623277	TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	669	0.3647923573547374	RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGACCTCGTATGCCGT	406	0.22138370266969112	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGAACTCGTATGCCGT	397	0.21647618216716102	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT	246	0.13413889373582272	No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC	238	0.1297766532891293	TruSeq Adapter, Index 5 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.005998080614203455	0.0
2	0.0	0.0	0.0	0.0065433606700401326	0.0
3	0.0	0.0	0.0	0.0065433606700401326	0.0
4	0.0	0.0	0.0	0.012541441284243587	0.0
5	0.0	0.0	0.0	0.013632001395916943	0.0
6	0.0	0.0	0.0	0.014722561507590298	0.0
7	0.0	0.0	0.0	0.015813121619263654	0.0
8	0.0	0.0	0.0	0.015813121619263654	0.0
9	0.0	0.0	0.0	0.015813121619263654	0.0
10	0.0	0.0	0.0	0.017448961786773687	0.0
11	0.0	0.0	0.0	0.017994241842610365	0.0
12	0.0	0.0	0.0	0.020175362065957075	0.0
13	0.0	0.0	0.0	0.020175362065957075	0.0
14	0.0	0.0	0.0	0.022356482289303786	0.0
15	0.0	0.0	0.0	0.02344704240097714	0.0
16	0.0	0.0	0.0	0.02344704240097714	0.0
17	0.0	0.0	0.0	0.02344704240097714	0.0
18	0.0	0.0	0.0	0.027264002791833886	0.0
19	0.0	0.0	0.0	0.029990403071017274	0.0
20	0.0	0.0	0.0	0.030535683126853952	0.0
21	0.0	0.0	0.0	0.039260164020240795	0.0
22	0.0	0.0	0.0	0.03980544407607747	0.0
23	0.0	0.0	0.0	0.041441284243587506	0.0
24	0.0	0.0	0.0	0.046348804746117606	0.0
25	0.0	0.0	0.0	0.05016576513697435	0.0
26	0.0	0.0	0.0	0.053982725527831094	0.0
27	0.0	0.0	0.0	0.07961088815215495	0.0
28	0.0	0.0	0.0	0.09978625021811202	0.0
29	0.0	0.0	0.0	0.1199616122840691	0.0
30	0.0	0.0	0.0	0.15758593613679986	0.0
31	0.0	0.0	0.0	0.20938754144128424	0.0
32	0.0	0.0	0.0	0.24919298551736171	0.0
33	0.0	0.0	0.0	0.29826819054266274	0.0
34	0.0	0.0	0.0	0.35933955679637064	0.0
35	0.0	0.0	0.0	0.45912580701448263	0.0
36	0.0	0.0	0.0	0.6494285465014832	0.0
37	0.0	0.0	0.0	0.9160704938056186	0.0
38	0.0	0.0	0.0	1.2432385273076252	0.0
39	0.0	0.0	0.0	1.6412929680683999	0.0
40	0.0	0.0	0.0	2.150584540219857	0.0
41	0.0	0.0	0.0	2.755845402198569	0.0
42	0.0	0.0	0.0	3.375283545629035	0.0
43	0.0	0.0	0.0	4.073787297155819	0.0
44	0.0	0.0	0.0	4.795192811027744	0.0
45	0.0	0.0	0.0	5.535137846798116	0.0
46	0.0	0.0	0.0	6.325248647705462	0.0
47	0.0	0.0	0.0	7.093002966323504	0.0
48	0.0	0.0	0.0	7.800231198743675	0.0
49	0.0	0.0	0.0	8.502006630605479	0.0
50	0.0	0.0	0.0	9.121444774035945	0.0
51	0.0	0.0	0.0	9.736520677019717	0.0
52	0.0	0.0	0.0	10.49773163496772	0.0
53	0.0	0.0	0.0	11.238767230849765	0.0
54	0.0	0.0	0.0	12.001614028965276	0.0
55	0.0	0.0	0.0	12.611237131390682	0.0
56	0.0	0.0	0.0	13.236673355435352	0.0
57	0.0	0.0	0.0	13.859928459256674	0.0
58	0.0	0.0	0.0	14.412297155819228	0.0
59	0.0	0.0	0.0	14.92758680858489	0.0
60	0.0	0.0	0.0	15.423791659396265	0.0
61	0.0	0.0	0.0	15.975069795847148	0.0
62	0.0	0.0	0.0	16.464731285988485	0.0
63	0.0	0.0	0.0	17.072173268190543	0.0
64	0.0	0.0	0.0	17.611455243413015	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCGTA	3435	0.0	53.884853	38
GATCTCG	3400	0.0	53.822086	36
ATCTCGT	3440	0.0	53.70482	37
CTTGAAA	3545	0.0	53.53974	57
CCGTCTT	3585	0.0	53.430763	47
ATGCCGT	3630	0.0	53.303566	44
CGTATGC	3685	0.0	53.267597	41
CTCGTAT	3620	0.0	53.2575	39
GTCTTCT	3620	0.0	53.20437	49
CGTCTTC	3575	0.0	53.188404	48
TGCCGTC	3635	0.0	53.162983	45
TCGTATG	3690	0.0	53.100597	40
GCCGTCT	3635	0.0	53.081154	46
TATGCCG	3655	0.0	53.034702	43
AGTGATC	3385	0.0	53.026924	33
CTTCTGC	3630	0.0	52.961338	51
TTGAAAA	3565	0.0	52.94469	58
TGAAAAA	3590	0.0	52.673542	59
GCTTGAA	3610	0.0	52.67273	56
CTGCTTG	3670	0.0	52.67035	54
>>END_MODULE