FastQCFastQC Report
Wed 25 May 2016
SRR1779814_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779814_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482530
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAA200.00694887152.501439
TCGGTGG1500.039.659545
GACGAGC1450.036.20410523
CGACGAG1900.031.30688922
TATTCCG450.004309816331.105495
ACGAATA802.4661676E-630.62264324
GACGACT604.9823016E-429.1644223
TAGATCT2700.028.51927839
CTCGGTG1850.028.37619244
GCCGTAC500.00719441428.00945754
TATCACG500.007208996527.99784344
GTTTACG500.00721629627.9920391
CGAATAG906.169521E-627.22012525
GACGAAT1056.7675137E-726.66461223
ACGAGCG808.664976E-526.24797824
CTTCCGA1207.419112E-826.23981718
GATCTCG1900.025.78748741
CTAGTAC700.001226814224.9954833
TCTCGGT1701.4006218E-1024.7039843
GTAGATC2600.024.23141538
Produced by FastQC (version 0.11.5)