##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779814_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	482530
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.13626717509792	34.0	31.0	34.0	12.0	34.0
2	31.039699086067188	34.0	32.0	34.0	21.0	34.0
3	30.571479493502995	34.0	32.0	34.0	21.0	34.0
4	30.724006797504817	34.0	32.0	34.0	21.0	34.0
5	31.33052245456241	34.0	34.0	34.0	23.0	34.0
6	34.007301100449716	38.0	34.0	38.0	22.0	38.0
7	34.23117111889416	38.0	35.0	38.0	23.0	38.0
8	33.367034174040995	38.0	34.0	38.0	21.0	38.0
9	34.295898700598926	38.0	35.0	38.0	23.0	38.0
10-11	32.88008517605123	37.0	31.0	38.0	17.0	38.0
12-13	33.88382380370132	38.0	34.0	38.0	21.5	38.0
14-15	32.58761631401157	37.0	32.5	38.0	16.0	38.0
16-17	33.31597724493814	37.5	32.5	38.0	17.0	38.0
18-19	34.27777236648499	38.0	35.0	38.0	23.0	38.0
20-21	32.83357615070565	37.0	30.5	38.0	17.0	38.0
22-23	33.83656974695874	38.0	35.0	38.0	22.0	38.0
24-25	34.135106625494785	38.0	35.0	38.0	22.5	38.0
26-27	32.98533044577539	38.0	33.0	38.0	16.0	38.0
28-29	34.31587466064286	38.0	35.0	38.0	23.5	38.0
30-31	34.74594222120905	38.0	36.0	38.0	25.0	38.0
32-33	34.84360972374775	38.0	36.5	38.0	26.0	38.0
34-35	34.75167865210453	38.0	36.5	38.0	25.5	38.0
36-37	34.23463618842352	38.0	35.5	38.0	22.5	38.0
38-39	34.173953951049675	38.0	35.0	38.0	22.5	38.0
40-41	34.186554203883695	38.0	35.0	38.0	22.5	38.0
42-43	34.57164321389344	38.0	36.0	38.0	24.5	38.0
44-45	34.629313203324145	38.0	36.0	38.0	24.5	38.0
46-47	34.53756968478643	38.0	36.0	38.0	24.0	38.0
48-49	34.26793981721344	38.0	35.0	38.0	23.0	38.0
50-51	33.601082834227924	38.0	34.5	38.0	17.0	38.0
52-53	34.165460178641744	38.0	34.5	38.0	23.0	38.0
54-55	34.32113236482705	38.0	35.5	38.0	23.5	38.0
56-57	34.386638136488926	38.0	36.0	38.0	23.5	38.0
58-59	34.64693386939672	38.0	36.0	38.0	24.0	38.0
60-61	34.495626178683196	38.0	36.0	38.0	24.0	38.0
62-63	34.53277309182849	38.0	36.0	38.0	24.0	38.0
64-65	34.52788531283029	38.0	36.0	38.0	23.5	38.0
66-67	34.3300748140012	38.0	36.0	38.0	23.0	38.0
68-69	33.69472364412575	38.0	34.0	38.0	21.5	38.0
70-71	33.72400265268481	38.0	34.0	38.0	21.5	38.0
72-73	33.99454541686527	38.0	34.0	38.0	22.5	38.0
74-75	34.06388411083249	38.0	35.0	38.0	22.5	38.0
76	32.98105195531884	37.0	32.0	38.0	21.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	35.0
13	593.0
14	2141.0
15	3794.0
16	5160.0
17	5879.0
18	5998.0
19	5524.0
20	4654.0
21	3830.0
22	3448.0
23	3445.0
24	3707.0
25	4182.0
26	4902.0
27	5873.0
28	7040.0
29	8593.0
30	10632.0
31	13359.0
32	16941.0
33	22337.0
34	31405.0
35	46490.0
36	83987.0
37	178576.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.00644519511741	17.19209168341865	10.896110086419498	28.905353035044453
2	23.444759911300853	20.841812944272895	29.323565374173626	26.389861770252626
3	21.01071435972893	20.891965266408306	28.99073632727499	29.106584046587773
4	15.181853977991006	18.18726296810561	30.028392017076662	36.60249103682672
5	17.26068845460386	31.01029987772781	32.21063975296873	19.518371914699603
6	31.1318173905114	35.600477497119265	16.411062016596343	16.85664309577299
7	29.399219464070686	29.31030551991859	18.440627661977786	22.849847354032942
8	25.8771229909951	35.36470368796825	17.59841662953483	21.15975669150182
9	25.406020460612496	17.75836912006102	18.510512178541227	38.32509824078526
10-11	25.72481676868348	24.51553670999143	23.33786165711781	26.421784864207282
12-13	25.892924681288914	21.915965759606408	24.399751725024068	27.79135783408061
14-15	25.028910239405793	23.54992870880032	22.00525150872074	29.41590954307315
16-17	25.87964171878821	25.06486697089898	22.532671814575796	26.522819495737014
18-19	23.803089243191376	24.772700424651774	24.75093935292935	26.673270979227503
20-21	24.95761921538557	24.351957391250284	24.6121484674528	26.078274925911344
22-23	25.826681574619727	24.5505594109217	23.45173688360916	26.171022130849416
24-25	24.408590451024583	26.156420818113517	22.926970803865185	26.508017926996708
26-27	24.579737236684903	25.949923890032757	23.744444594119894	25.725894279162446
28-29	24.117298381748242	25.553165207587213	24.530151545297784	25.799384865366758
30-31	24.49901819067307	25.316380931656745	24.258410142427113	25.926190735243072
32-33	23.671274324912442	25.860050152322135	24.611526744451123	25.857148778314304
34-35	23.66178939296595	25.982259435037612	24.56083811734472	25.79511305465172
36-37	24.817355597173872	25.052520953969154	24.23809637322991	25.892027075627066
38-39	24.83429227567796	24.357895522264318	25.190890324078296	25.616921877979422
40-41	23.56880620816931	24.847909592342788	25.299077458082525	26.284206741405374
42-43	22.608301893048804	24.248205028304028	26.166122830050455	26.977370248596717
44-45	22.326515470241883	23.46421917194984	27.326235681554877	26.8830296762534
46-47	22.132379990155695	23.86995155565918	27.438563767778035	26.559104686407085
48-49	21.773072941654345	24.39415621221086	27.433037772820107	26.399733073314692
50-51	22.696849965338117	24.695529808701636	26.378055970910957	26.229564255049286
52-53	20.300790840305392	25.599451976997127	26.86742880001824	27.23232838267924
54-55	20.58512633762853	25.036944766279014	26.752156061325206	27.625772834767254
56-57	20.810105802504065	25.268340810706896	26.342636782045183	27.57891660474386
58-59	20.784819702881542	24.774207589112223	26.77672589345396	27.664246814552275
60-61	20.628551942445448	24.650467615198764	26.611932764573393	28.109047677782396
62-63	21.484230478655984	24.70912427935262	25.784641596134854	28.022003645856543
64-65	21.86847983250328	23.950580175063095	25.28920651537373	28.891733477059894
66-67	21.62387652560361	25.572412277792516	24.281764062085433	28.521947134518438
68-69	22.047563931324408	25.92256017507738	23.6379330175668	28.39194287603141
70-71	22.602418926984548	26.403649114662777	23.414081669872008	27.579850288480667
72-73	23.247268416427637	26.792834878052822	22.875665279458822	27.084231426060718
74-75	24.034865368732053	27.16190882815925	22.411704417447606	26.391521385661093
76	23.633281244171055	27.375203624410887	22.611739543135215	26.37977558828284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	1.5
10	3.5
11	6.5
12	7.0
13	12.5
14	25.5
15	53.5
16	109.0
17	144.0
18	179.5
19	297.0
20	432.5
21	486.0
22	602.0
23	848.0
24	1180.5
25	1383.0
26	1599.0
27	2118.0
28	2792.0
29	3163.0
30	3694.0
31	4713.0
32	5830.5
33	6460.0
34	7300.5
35	8803.0
36	10235.0
37	11005.0
38	12235.0
39	14375.0
40	16353.0
41	18228.5
42	19036.0
43	19762.5
44	21087.5
45	22460.5
46	23235.0
47	23452.0
48	23576.5
49	23409.5
50	23335.0
51	23104.5
52	22316.5
53	21457.5
54	21156.0
55	20388.5
56	18931.5
57	17248.0
58	16254.0
59	15482.5
60	13614.0
61	11856.5
62	11196.0
63	10350.5
64	8832.5
65	7593.0
66	6704.0
67	6382.0
68	5886.5
69	5025.0
70	4341.0
71	4023.0
72	3849.0
73	3452.5
74	3038.5
75	2847.0
76	2529.0
77	2099.5
78	1876.0
79	1764.0
80	1570.0
81	1246.5
82	1036.0
83	955.0
84	852.0
85	645.5
86	444.5
87	347.0
88	308.5
89	205.5
90	129.5
91	107.5
92	97.0
93	73.5
94	47.5
95	36.5
96	28.0
97	22.5
98	18.0
99	21.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0029013740078337098
7	0.008496881022941579
8	0.003108615008393261
9	0.00870412202350113
10-11	0.002383271506434833
12-13	1.0362050027977534E-4
14-15	4.144820011191014E-4
16-17	8.289640022382027E-4
18-19	0.0035230970095123616
20-21	0.0
22-23	0.0027977535075539343
24-25	0.0036267175097921375
26-27	9.325845025179782E-4
28-29	0.007046194019024723
30-31	5.181025013988767E-4
32-33	0.0
34-35	0.004144820011191014
36-37	0.0214494435579135
38-39	0.011191014030215737
40-41	9.325845025179782E-4
42-43	3.1086150083932607E-4
44-45	0.004766543012869666
46-47	0.0036267175097921375
48-49	1.0362050027977534E-4
50-51	0.002383271506434833
52-53	0.014196008538329224
54-55	0.010983773029656188
56-57	0.015439454541686526
58-59	0.012952562534971918
60-61	0.02808115557581912
62-63	0.013367044536091019
64-65	0.027459432574140467
66-67	0.020309618054835967
68-69	1.0362050027977534E-4
70-71	4.144820011191014E-4
72-73	0.005388266014548318
74-75	0.009429465525459556
76	0.004973784013429217
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	482530.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.04283569340073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.87894428818566	45.216163659804096
2	11.333198483425894	14.062875427757824
3	7.219116713252208	13.436834162753694
4	4.180315937576693	10.374346194463008
5	2.2243327116942333	6.900195447955092
6	1.0689913773661999	3.9793953830155964
7	0.4977971912459349	2.1619324541575544
8	0.23269358361477882	1.154957582009626
9	0.11081196882439287	0.6187579897170645
>10	0.2534608350878891	2.08262708254226
>50	3.369097260897934E-4	0.01191461582419969
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0012434460033573043	0.0
2	0.0	0.0	0.0	0.002694133007274159	0.0
3	0.0	0.0	0.0	0.002694133007274159	0.0
4	0.0	0.0	0.0	0.004973784013429217	0.0
5	0.0	0.0	0.0	0.006009989016226971	0.0
6	0.0	0.0	0.0	0.007253435019584274	0.0
7	0.0	0.0	0.0	0.007253435019584274	0.0
8	0.0	0.0	0.0	0.007460676020143825	0.0
9	0.0	0.0	0.0	0.009533086025739332	0.0
10	0.0	0.0	0.0	0.010154809027417984	0.0
11	0.0	0.0	0.0	0.012227219033013492	0.0
12	0.0	0.0	0.0	0.014299629038609	0.0
13	0.0	0.0	0.0	0.0147141110397281	0.0
14	0.0	0.0	0.0	0.016786521045323608	0.0
15	0.0	0.0	0.0	0.016993762045883157	0.0
16	0.0	0.0	0.0	0.018444449049800013	0.0
17	0.0	0.0	0.0	0.019480654052597766	0.0
18	0.0	0.0	0.0	0.02072410005595507	0.0
19	0.0	0.0	0.0	0.02341823306322923	0.0
20	0.0	0.0	0.0	0.028599258077218	0.0
21	0.0	0.0	0.0	0.03129339108449215	0.0
22	0.0	0.0	0.0	0.03626717509792137	0.0
23	0.0	0.0	0.0	0.03833958510351688	0.0
24	0.0	0.0	0.0	0.043313369116946096	0.0
25	0.0	0.0	0.0	0.04704370712701801	0.0
26	0.0	0.0	0.0	0.0596854081611506	0.0
27	0.0	0.0	0.0	0.14154560338217312	0.0
28	0.0	0.0	0.0	0.1896255155119889	0.0
29	0.0	0.0	0.0	0.2459950676641867	0.0
30	0.0	0.0	0.0	0.2990487638074317	0.0
31	0.0	0.0	0.0	0.34899384494228336	0.0
32	0.0	0.0	0.0	0.40391271009056434	0.0
33	0.0	0.0	0.0	0.47520361428304975	0.0
34	0.0	0.0	0.0	0.5713634385426812	0.0
35	0.0	0.0	0.0	0.7236855739539511	0.0
36	0.0	0.0	0.0	1.033718110791039	0.0
37	0.0	0.0	0.0	1.5686071332352394	0.0
38	0.0	0.0	0.0	2.225975587010134	0.0
39	0.0	0.0	0.0	2.9554639089797528	0.0
40	0.0	0.0	0.0	3.7769672351978114	0.0
41	0.0	0.0	0.0	4.8278863490352935	0.0
42	0.0	0.0	0.0	5.99175180817773	0.0
43	0.0	0.0	0.0	7.068783288085715	0.0
44	0.0	0.0	0.0	8.073073176797298	0.0
45	0.0	0.0	0.0	9.08254409052287	0.0
46	0.0	0.0	0.0	10.074606760201439	0.0
47	0.0	0.0	0.0	11.009678154726132	0.0
48	0.0	0.0	0.0	11.939361283236275	0.0
49	0.0	0.0	0.0	12.67071477421093	0.0
50	0.0	0.0	0.0	13.479990881395976	0.0
51	0.0	0.0	0.0	14.349159637742732	0.0
52	0.0	0.0	0.0	15.38329223053489	0.0
53	0.0	0.0	0.0	16.437112718380206	0.0
54	0.0	0.0	0.0	17.345242782832155	0.0
55	0.0	0.0	0.0	18.20467121215261	0.0
56	0.0	0.0	0.0	19.0311483223841	0.0
57	0.0	0.0	0.0	19.802706567467308	0.0
58	0.0	0.0	0.0	20.529500756429652	0.0
59	0.0	0.0	0.0	21.142519636084803	0.0
60	0.0	0.0	0.0	21.77916398980374	0.0
61	0.0	0.0	0.0	22.41352869251653	0.0
62	0.0	0.0	0.0	23.101568814374236	0.0
63	0.0	0.0	0.0	23.820280604314757	0.0
64	0.0	0.0	0.0	24.521376909207717	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAA	20	0.006948871	52.5014	39
TCGGTGG	150	0.0	39.6595	45
GACGAGC	145	0.0	36.204105	23
CGACGAG	190	0.0	31.306889	22
TATTCCG	45	0.0043098163	31.10549	5
ACGAATA	80	2.4661676E-6	30.622643	24
GACGACT	60	4.9823016E-4	29.16442	23
TAGATCT	270	0.0	28.519278	39
CTCGGTG	185	0.0	28.376192	44
GCCGTAC	50	0.007194414	28.009457	54
TATCACG	50	0.0072089965	27.997843	44
GTTTACG	50	0.007216296	27.992039	1
CGAATAG	90	6.169521E-6	27.220125	25
GACGAAT	105	6.7675137E-7	26.664612	23
ACGAGCG	80	8.664976E-5	26.247978	24
CTTCCGA	120	7.419112E-8	26.239817	18
GATCTCG	190	0.0	25.787487	41
CTAGTAC	70	0.0012268142	24.995483	3
TCTCGGT	170	1.4006218E-10	24.70398	43
GTAGATC	260	0.0	24.231415	38
>>END_MODULE