FastQCFastQC Report
Wed 25 May 2016
SRR1779814_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779814_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482530
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT257655.3395643794168235No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG32890.6816156508403622No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC17960.37220483700495305No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT7510.15563799142022258RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC6250.12952562534971918No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAT150.00222834369.9665234
CGTCTTC38200.053.37100648
CCGTCTT38550.053.1480347
ATGCCGT38700.052.97207344
CGTATGC38850.052.86307541
TGCCGTC38850.052.8113345
TATGCCG38850.052.77301843
GCCGTCT39000.052.7089246
TCGTATG39050.052.59232740
ACAAACG200.00693142452.5347847
TCTCGTA38100.052.3425938
CTCGTAT38850.052.32273539
GTATGCC39400.052.03634342
GTCTTCT39400.051.92870749
ATCTCGT38400.051.84255237
AATCTCG38350.051.72232436
TCTTCTG39950.051.12609550
CGTCAAT39150.051.02284232
CTGCTTG39900.050.7511454
GTCAATC39150.050.48670233