##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779814_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	482530
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.59008351812322	34.0	34.0	34.0	34.0	34.0
2	33.67226700930512	34.0	34.0	34.0	34.0	34.0
3	33.76937392493731	34.0	34.0	34.0	34.0	34.0
4	33.591324891716575	34.0	34.0	34.0	34.0	34.0
5	33.62084222742627	34.0	34.0	34.0	34.0	34.0
6	37.290400596854084	38.0	38.0	38.0	37.0	38.0
7	37.3562597144219	38.0	38.0	38.0	37.0	38.0
8	37.26916668393675	38.0	38.0	38.0	36.0	38.0
9	37.18517397881997	38.0	38.0	38.0	36.0	38.0
10-11	37.15019791515553	38.0	38.0	38.0	36.0	38.0
12-13	37.29809856381986	38.0	38.0	38.0	36.5	38.0
14-15	37.307213023024474	38.0	38.0	38.0	36.5	38.0
16-17	37.26830041655441	38.0	38.0	38.0	36.0	38.0
18-19	37.1563716245622	38.0	38.0	38.0	36.0	38.0
20-21	37.14819389468012	38.0	38.0	38.0	36.0	38.0
22-23	37.11189252481711	38.0	38.0	38.0	36.0	38.0
24-25	37.305102273433775	38.0	38.0	38.0	36.5	38.0
26-27	37.226176610780676	38.0	38.0	38.0	36.0	38.0
28-29	36.782822829668625	38.0	37.0	38.0	35.0	38.0
30-31	37.08853335543904	38.0	38.0	38.0	36.0	38.0
32-33	37.11982052929352	38.0	38.0	38.0	36.0	38.0
34-35	37.18030899633183	38.0	38.0	38.0	36.0	38.0
36-37	37.13932812467618	38.0	38.0	38.0	36.0	38.0
38-39	36.88961618966697	38.0	38.0	38.0	35.5	38.0
40-41	36.971635960458414	38.0	38.0	38.0	35.5	38.0
42-43	37.19050318114936	38.0	38.0	38.0	36.0	38.0
44-45	36.95598097527615	38.0	38.0	38.0	35.5	38.0
46-47	37.09517232089196	38.0	38.0	38.0	36.0	38.0
48-49	36.77310529915239	38.0	37.0	38.0	35.0	38.0
50-51	36.9517418606097	38.0	37.5	38.0	35.5	38.0
52-53	36.867637245352626	38.0	38.0	38.0	35.5	38.0
54-55	36.86358568379168	38.0	37.5	38.0	35.5	38.0
56-57	36.745389923942554	38.0	37.0	38.0	35.0	38.0
58-59	36.84301390587113	38.0	37.5	38.0	35.5	38.0
60-61	36.5606304271237	38.0	37.0	38.0	34.0	38.0
62-63	36.3648508901001	38.0	37.0	38.0	34.0	38.0
64-65	36.665677781692324	38.0	37.0	38.0	34.5	38.0
66-67	36.482978260419046	38.0	37.0	38.0	34.0	38.0
68-69	36.605721924025445	38.0	37.0	38.0	34.0	38.0
70-71	36.201853770750006	38.0	37.0	38.0	32.5	38.0
72-73	35.48424139431745	38.0	37.0	38.0	28.5	38.0
74-75	35.3937775889582	38.0	37.0	38.0	27.5	38.0
76	35.096412658280315	38.0	37.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	9.0
22	30.0
23	69.0
24	151.0
25	263.0
26	518.0
27	834.0
28	1440.0
29	2255.0
30	3586.0
31	5696.0
32	8894.0
33	14261.0
34	23399.0
35	43851.0
36	72913.0
37	304359.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.51384528613315	15.340129142492193	9.988636787777496	31.157388783597163
2	22.217478705987194	19.742399436304478	33.043126852216446	24.996995005491886
3	21.376494725716537	18.61148529625101	32.27011792012932	27.741902057903133
4	15.063519366671501	20.26402503471287	31.792012931838435	32.88044266677719
5	15.426605599651836	29.811203448490247	36.99438376888484	17.76780718297308
6	31.012372287733402	36.43877064638468	17.39871096097652	15.150146104905392
7	29.183263216794806	27.111060452199865	18.62992974530081	25.07574658570452
8	25.867407207841996	36.07216131639483	18.008828466623836	20.051603009139328
9	24.64820840155016	17.001637203904423	18.392638799660123	39.95751559488529
10-11	25.36795639649348	25.533334714940004	25.923465898493358	23.175242990073155
12-13	25.629805400700473	20.284334652767704	26.1983710857356	27.88748886079622
14-15	23.396576378670755	21.057861687356226	21.141483431082005	34.40407850289101
16-17	26.804965494373405	26.559903011211738	21.67502538702257	24.960106107392285
18-19	26.57192298924419	23.77883240420285	23.26311317431041	26.386131432242554
20-21	23.61635545976416	22.98965867407208	23.018983275651255	30.375002590512505
22-23	23.62060390027563	26.391830559757945	22.673408907218203	27.314156632748222
24-25	26.986166519869435	26.532925755142223	22.471892648049323	24.00901507693902
26-27	26.88247362858268	26.451930449920212	23.060535096263447	23.605060825233664
28-29	23.306426543427353	23.511077031479907	26.43949598988664	26.7430004352061
30-31	23.254098190786067	22.709468841315566	30.418005098128614	23.618427869769757
32-33	25.687314778355752	23.175242990073155	24.395996103869187	26.741446127701906
34-35	23.719250616541977	22.794126790044142	26.965577269807056	26.521045323606824
36-37	22.932252916917083	29.09021200754357	23.832300582347212	24.145234493192135
38-39	22.858993223219283	23.775827409694735	27.009305120925127	26.35587424616086
40-41	22.47808426419083	22.05614158705158	28.071000766791705	27.394773381965887
42-43	25.447849658465415	21.64610136613834	28.182924099741363	24.723124875654882
44-45	22.158000538826602	24.606449339937413	29.251859987980023	23.983690133255962
46-47	24.705925020206	21.54518890017201	26.799888089859696	26.948997989762297
48-49	24.037054690900046	21.831285101444468	26.3372225561105	27.794437651544985
50-51	21.024704037607925	22.377378866062184	29.393109981003274	27.204807115326616
52-53	19.84163592432772	22.65607589202883	32.59824677969896	24.904041403944486
54-55	19.577471651908137	22.823622355828295	29.641368256150425	27.957537736113142
56-57	22.57304957479766	22.535620156726615	26.47099686671187	28.42033340176385
58-59	19.653164210543775	22.218306063043713	31.992797492183804	26.135732234228705
60-61	22.695208961984957	25.363300601121384	26.285662752138755	25.6558276847549
62-63	19.72121217146861	29.7051665305344	24.298262344156193	26.275358953840794
64-65	19.66719212006455	30.07987513486119	23.40226411339844	26.850668631675823
66-67	19.62797987724414	30.210463791582487	22.62534998688683	27.536206344286544
68-69	19.74167045780745	30.491342208920702	22.700211094864187	27.06677623840766
70-71	19.883017299718425	29.598861677905834	22.919613774692234	27.598507247683507
72-73	20.22978855721393	28.084266169154226	23.652985074626866	28.032960199004975
74-75	20.498468260449616	27.33471100075032	24.105414357311933	28.06140638148813
76	20.21220587991444	26.78005869965344	24.568250783491134	28.439484636940985
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.5
8	2.0
9	1.5
10	0.5
11	1.5
12	3.0
13	4.0
14	17.0
15	50.0
16	92.5
17	114.0
18	146.5
19	234.5
20	375.0
21	460.0
22	592.5
23	796.0
24	1142.5
25	1418.0
26	1688.5
27	2318.5
28	2948.5
29	3219.0
30	3699.5
31	4747.5
32	5928.5
33	6542.0
34	7249.5
35	8688.0
36	10002.0
37	10585.0
38	11784.0
39	17760.0
40	25059.0
41	27032.0
42	26483.0
43	24798.0
44	22392.5
45	21657.5
46	21643.0
47	21716.0
48	21026.5
49	20213.5
50	20163.0
51	20066.5
52	19364.0
53	18919.0
54	19080.0
55	18547.5
56	17460.0
57	16210.5
58	15516.0
59	14580.5
60	12813.0
61	11112.0
62	10243.0
63	9721.5
64	8585.0
65	7346.0
66	6222.5
67	5723.0
68	5541.0
69	4926.5
70	4319.5
71	4145.0
72	3838.5
73	3236.0
74	2821.5
75	2703.0
76	2542.5
77	2166.0
78	1861.5
79	1773.0
80	1580.5
81	1275.0
82	1004.0
83	846.0
84	733.0
85	525.5
86	376.0
87	321.0
88	286.0
89	196.0
90	98.0
91	49.5
92	44.0
93	26.0
94	7.0
95	4.5
96	3.0
97	2.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.056369552152197785
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0010362050027977535
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	4.144820011191014E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.01626841854392473
52-53	0.033262180589807885
54-55	0.05522972664912026
56-57	0.05989264916171016
58-59	0.04103371811079104
60-61	0.037614241601558455
62-63	0.03232959608728991
64-65	0.018548069550079788
66-67	0.039479410606594406
68-69	0.009429465525459556
70-71	0.013574285536650571
72-73	0.026941330072741593
74-75	0.01492135204028765
76	0.015335834041406753
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	482530.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.669480000955808
#Duplication Level	Percentage of deduplicated	Percentage of total
1	32.440984876008066	9.949481368660392
2	17.876151299453742	10.965045295453136
3	16.057882491503207	14.774607177925681
4	12.804861278756263	15.708737476153145
5	8.791557257820262	13.48162447479882
6	5.32693249614676	9.802454979420881
7	2.9384841820496077	6.30852472981468
8	1.5686313318958318	3.848728580996151
9	0.8376514111514071	2.312129988187244
>10	1.3398226757493568	5.577218679352647
>50	0.006816279786186672	0.14654528136493264
>100	0.006816279786184576	0.3880000602186182
>500	0.0013632559572369152	0.28765521705863073
>1k	0.0013632559572369152	1.0630281822261172
>5k	0.0	0.0
>10k+	6.816279786184576E-4	5.38621850836891
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT	25765	5.3395643794168235	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG	3289	0.6816156508403622	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	1796	0.37220483700495305	No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT	751	0.15563799142022258	RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC	625	0.12952562534971918	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0014506870039168549	0.0
2	0.0	0.0	0.0	0.0033158560089528114	0.0
3	0.0	0.0	0.0	0.0033158560089528114	0.0
4	0.0	0.0	0.0	0.006631712017905623	0.0
5	0.0	0.0	0.0	0.007667917020703376	0.0
6	0.0	0.0	0.0	0.00891136302406068	0.0
7	0.0	0.0	0.0	0.00891136302406068	0.0
8	0.0	0.0	0.0	0.00911860402462023	0.0
9	0.0	0.0	0.0	0.011191014030215737	0.0
10	0.0	0.0	0.0	0.012848942034692144	0.0
11	0.0	0.0	0.0	0.016993762045883157	0.0
12	0.0	0.0	0.0	0.020931341056514622	0.0
13	0.0	0.0	0.0	0.021760305058752823	0.0
14	0.0	0.0	0.0	0.024661679066586534	0.0
15	0.0	0.0	0.0	0.024868920067146083	0.0
16	0.0	0.0	0.0	0.027148571073301143	0.0
17	0.0	0.0	0.0	0.028184776076098896	0.0
18	0.0	0.0	0.0	0.0294282220794562	0.0
19	0.0	0.0	0.0	0.03191511408617081	0.0
20	0.0	0.0	0.0	0.03771786210183823	0.0
21	0.0	0.0	0.0	0.040619236109671934	0.0
22	0.0	0.0	0.0	0.04227716411414834	0.0
23	0.0	0.0	0.0	0.0458002611236607	0.0
24	0.0	0.0	0.0	0.048079912129815765	0.0
25	0.0	0.0	0.0	0.05181025013988767	0.0
26	0.0	0.0	0.0	0.06548815617681802	0.0
27	0.0	0.0	0.0	0.15315109941350796	0.0
28	0.0	0.0	0.0	0.2039251445505979	0.0
29	0.0	0.0	0.0	0.2621598657078316	0.0
30	0.0	0.0	0.0	0.3174932128572317	0.0
31	0.0	0.0	0.0	0.37179035500383395	0.0
32	0.0	0.0	0.0	0.4304395581621868	0.0
33	0.0	0.0	0.0	0.5054608003647442	0.0
34	0.0	0.0	0.0	0.6099102646467577	0.0
35	0.0	0.0	0.0	0.772594450086005	0.0
36	0.0	0.0	0.0	1.1072886659896795	0.0
37	0.0	0.0	0.0	1.6672538495015854	0.0
38	0.0	0.0	0.0	2.3519781153503407	0.0
39	0.0	0.0	0.0	3.0947298613557708	0.0
40	0.0	0.0	0.0	3.9614117256958115	0.0
41	0.0	0.0	0.0	5.049219737632893	0.0
42	0.0	0.0	0.0	6.254947878888359	0.0
43	0.0	0.0	0.0	7.37549996891385	0.0
44	0.0	0.0	0.0	8.409632561706008	0.0
45	0.0	0.0	0.0	9.4553706505295	0.0
46	0.0	0.0	0.0	10.492404617329493	0.0
47	0.0	0.0	0.0	11.447578388908461	0.0
48	0.0	0.0	0.0	12.415601102522123	0.0
49	0.0	0.0	0.0	13.18778107060701	0.0
50	0.0	0.0	0.0	14.033531593890535	0.0
51	0.0	0.0	0.0	14.918450666279817	0.0
52	0.0	0.0	0.0	15.96418875510331	0.0
53	0.0	0.0	0.0	17.018216483949185	0.0
54	0.0	0.0	0.0	17.92862619940729	0.0
55	0.0	0.0	0.0	18.796965991751808	0.0
56	0.0	0.0	0.0	19.62572275298945	0.0
57	0.0	0.0	0.0	20.4155182061219	0.0
58	0.0	0.0	0.0	21.15288168611278	0.0
59	0.0	0.0	0.0	21.78434501481773	0.0
60	0.0	0.0	0.0	22.428242803556255	0.0
61	0.0	0.0	0.0	23.0715188692931	0.0
62	0.0	0.0	0.0	23.764325534163678	0.0
63	0.0	0.0	0.0	24.50210349615568	0.0
64	0.0	0.0	0.0	25.233249746129776	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCGAT	15	0.002228343	69.96652	34
CGTCTTC	3820	0.0	53.371006	48
CCGTCTT	3855	0.0	53.14803	47
ATGCCGT	3870	0.0	52.972073	44
CGTATGC	3885	0.0	52.863075	41
TGCCGTC	3885	0.0	52.81133	45
TATGCCG	3885	0.0	52.773018	43
GCCGTCT	3900	0.0	52.70892	46
TCGTATG	3905	0.0	52.592327	40
ACAAACG	20	0.006931424	52.53478	47
TCTCGTA	3810	0.0	52.34259	38
CTCGTAT	3885	0.0	52.322735	39
GTATGCC	3940	0.0	52.036343	42
GTCTTCT	3940	0.0	51.928707	49
ATCTCGT	3840	0.0	51.842552	37
AATCTCG	3835	0.0	51.722324	36
TCTTCTG	3995	0.0	51.126095	50
CGTCAAT	3915	0.0	51.022842	32
CTGCTTG	3990	0.0	50.75114	54
GTCAATC	3915	0.0	50.486702	33
>>END_MODULE