FastQCFastQC Report
Wed 25 May 2016
SRR1779813_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779813_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183635
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAG150.00222246769.9956423
CAATTTA252.3864614E-455.9965147
GACGAAT452.6882844E-854.4410523
CTCGGTG653.6379788E-1253.85747544
CGCGTTA200.00693700652.51103650
TCGTCTC200.006944488752.4967277
TAGATCC407.452818E-752.49672739
CGACGGA200.006951977552.48242622
TCGCTGC200.006951977552.48242615
GGTACGG200.006959472352.4681361
ACGAAGG552.2700988E-950.90592224
TCGGTGG707.2759576E-1249.9832745
TGGTCGC651.8371793E-1048.47172549
ACGATAT305.868227E-446.6637624
TTCCGAC604.9167284E-946.6510519
GTAGATC1400.044.997238
CGTACCA404.566612E-543.77112656
GATCTCG1050.043.3424441
ATCTCGG1150.042.61765342
TCTCGGT855.638867E-1141.18512743
Produced by FastQC (version 0.11.5)