##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779813_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	183635
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.960530399978218	34.0	23.0	34.0	12.0	34.0
2	29.54893674953032	34.0	30.0	34.0	12.0	34.0
3	29.20968225011572	34.0	27.0	34.0	11.0	34.0
4	29.572870095570018	34.0	27.0	34.0	12.0	34.0
5	29.856389032591824	34.0	31.0	34.0	11.0	34.0
6	32.48619816483786	37.0	31.0	38.0	12.0	38.0
7	32.562599722275166	37.0	31.0	38.0	12.0	38.0
8	31.961548724371717	37.0	28.0	38.0	11.0	38.0
9	32.906668118822665	37.0	34.0	38.0	12.0	38.0
10-11	31.57421787785553	37.0	28.5	38.0	11.5	38.0
12-13	32.428224467013365	37.0	31.0	38.0	11.0	38.0
14-15	31.22531107904267	37.0	27.0	38.0	11.0	38.0
16-17	31.841756745718406	37.0	28.5	38.0	11.0	38.0
18-19	32.63551610531761	37.5	32.0	38.0	11.0	38.0
20-21	31.44663598987121	37.0	27.5	38.0	11.0	38.0
22-23	32.3151850137501	37.0	31.0	38.0	11.0	38.0
24-25	32.602711901326	37.5	32.0	38.0	11.0	38.0
26-27	31.543793394505407	37.0	26.5	38.0	11.0	38.0
28-29	32.686511285974895	37.5	32.5	38.0	11.0	38.0
30-31	33.070618346175834	38.0	34.0	38.0	11.0	38.0
32-33	33.18172189397446	38.0	34.0	38.0	11.0	38.0
34-35	33.146276581261745	38.0	34.0	38.0	11.0	38.0
36-37	32.67719116726114	38.0	32.5	38.0	11.0	38.0
38-39	32.59411876820867	38.0	32.5	38.0	11.0	38.0
40-41	32.598772020584306	38.0	32.5	38.0	11.0	38.0
42-43	32.98780733520299	38.0	33.5	38.0	11.0	38.0
44-45	33.05225583358292	38.0	34.0	38.0	11.0	38.0
46-47	32.94973997331664	38.0	34.0	38.0	11.0	38.0
48-49	32.68802515860266	38.0	32.5	38.0	11.0	38.0
50-51	32.06104228496746	37.5	29.5	38.0	11.0	38.0
52-53	32.61301222533831	37.0	32.5	38.0	11.0	38.0
54-55	32.75434421542734	37.5	33.0	38.0	11.0	38.0
56-57	32.792711084488246	38.0	33.5	38.0	11.0	38.0
58-59	33.00926838565633	38.0	34.0	38.0	11.0	38.0
60-61	32.93468293081384	38.0	34.0	38.0	11.0	38.0
62-63	32.917453099899255	38.0	34.0	38.0	11.0	38.0
64-65	32.9256922699921	38.0	34.0	38.0	11.0	38.0
66-67	32.82794401938628	38.0	33.0	38.0	11.0	38.0
68-69	32.22662074223324	37.0	31.0	38.0	11.0	38.0
70-71	32.309840172080484	37.0	31.0	38.0	11.0	38.0
72-73	32.497838102758195	37.0	31.5	38.0	11.0	38.0
74-75	32.49773191385084	37.5	32.0	38.0	11.0	38.0
76	31.610575326054402	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	33.0
13	485.0
14	1823.0
15	3392.0
16	4465.0
17	4861.0
18	4831.0
19	4305.0
20	3243.0
21	2439.0
22	1861.0
23	1569.0
24	1449.0
25	1525.0
26	1630.0
27	1869.0
28	2161.0
29	2795.0
30	3395.0
31	4381.0
32	5612.0
33	7647.0
34	10872.0
35	16453.0
36	29651.0
37	60884.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.739537669834185	23.675225311079043	9.905519100389359	28.679717918697417
2	19.16519182073134	21.2497617556566	30.230620524409836	29.354425899202223
3	20.01198028698233	21.129414327334114	30.794783129577695	28.063822256105865
4	13.33460396983146	21.694666049500366	33.28178179540937	31.688948185258802
5	16.67764859640047	32.50633049255317	33.72614153075394	17.089879380292427
6	26.77137053515512	36.933708727924234	16.375409112939675	19.919511623980963
7	26.219857538066087	28.05454505848781	22.132245627028556	23.59335177641754
8	23.100507547868517	34.358594549850785	17.9667588820877	24.574139020192998
9	24.64804457103645	17.328460872359315	18.773316196212768	39.250178360391466
10-11	22.755306258594167	26.12326585070319	24.648541204340308	26.472886686362333
12-13	22.680378687011427	22.83312776194016	26.326752325951823	28.15974122509659
14-15	24.193710876768797	24.81913801600462	22.96845091744743	28.018700189779157
16-17	25.43728285611597	26.112266791552763	23.108738033261815	25.341712319069455
18-19	22.280279473509374	25.76335150383105	25.57438558848996	26.38198343416961
20-21	23.403490619979852	25.43578293898222	25.19753859558363	25.9631878454543
22-23	25.522442922685258	25.148598423481683	23.873225055477654	25.455733598355412
24-25	22.203252763051488	28.231728734918597	23.706832000479228	25.858186501550684
26-27	23.056397354527608	28.118082386479664	24.970797970969507	23.85472228802322
28-29	22.513921603333014	27.346794287036914	26.00678039947173	24.132503710158346
30-31	22.85327648127122	26.550566478521194	26.024521535985883	24.5716355042217
32-33	21.36804731150005	27.96241436770968	25.942908176045833	24.726630144744437
34-35	21.247403058876703	29.06232386408502	25.548454905121453	24.14181817191682
36-37	23.700005991383303	26.15783482300909	25.17443095475416	24.967728230853446
38-39	23.483519954688045	25.518201028232834	26.86557816312304	24.13270085395608
40-41	21.532312082382603	26.582385327096187	26.433989903665505	25.451312686855704
42-43	20.270921541666098	25.93086735735777	27.180918410412104	26.61729269056403
44-45	19.739794362392715	24.67624057857361	29.103221801071754	26.48074325796192
46-47	20.33147907630227	25.431094338389652	28.311319136422675	25.926107448885404
48-49	20.46924861409107	26.14657416382587	27.802313297101843	25.581863924981214
50-51	22.47087909993411	26.050340084190577	26.05197379527422	25.426807020601096
52-53	18.351864105852783	27.480550177410702	27.067998812723467	27.09958690401305
54-55	18.880296264023528	26.060069709182006	27.383727262825403	27.675906763969067
56-57	19.417015691122888	26.719024873779322	26.725560572309337	27.138398862788453
58-59	19.398302961647804	26.476194625682137	26.69513217946344	27.43037023320662
60-61	18.79430789133247	25.62565534145843	27.00347245863689	28.576564308572205
62-63	19.822128306161517	25.797114078059856	26.202308628628845	28.17844898714978
64-65	20.548504820523995	25.360586088566915	25.289503785609234	28.801405305299856
66-67	19.387527233115467	27.988017429193903	23.97140522875817	28.653050108932458
68-69	20.271627998159378	28.63856540337139	23.48591079514359	27.603895803325646
70-71	20.945353554605603	29.199226726931137	23.201731696027444	26.653688022435812
72-73	21.834420225728717	29.541599161345662	22.293229499938736	26.33075111298689
74-75	22.65963037694798	29.30380487911142	22.03387984391636	26.002684900024235
76	22.03277224433783	29.910527089652618	22.309413987834297	25.74728667817525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.0
12	0.0
13	2.5
14	3.5
15	5.5
16	10.5
17	12.0
18	28.5
19	61.0
20	93.0
21	109.0
22	149.5
23	215.5
24	325.0
25	409.0
26	530.0
27	749.0
28	943.0
29	1039.0
30	1267.5
31	1680.0
32	2291.5
33	2719.0
34	3096.0
35	3838.5
36	4634.0
37	5064.0
38	5588.5
39	6743.0
40	8073.0
41	9056.0
42	9339.0
43	9783.5
44	10531.0
45	11124.5
46	11415.0
47	11470.0
48	11284.0
49	10635.0
50	10227.0
51	9873.5
52	8796.5
53	7580.0
54	7087.0
55	6596.5
56	5691.0
57	4811.0
58	4346.0
59	3979.0
60	3263.0
61	2755.5
62	2597.0
63	2336.5
64	1945.5
65	1688.0
66	1484.5
67	1408.0
68	1343.5
69	1189.5
70	987.0
71	874.0
72	857.5
73	760.5
74	645.5
75	611.0
76	557.0
77	476.5
78	395.5
79	341.0
80	329.5
81	274.5
82	201.0
83	171.0
84	162.0
85	134.0
86	92.0
87	69.0
88	60.0
89	40.5
90	30.0
91	30.5
92	31.0
93	20.0
94	6.5
95	6.0
96	8.0
97	6.5
98	6.5
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0032673509951806575
7	0.0038119094943774336
8	0.0038119094943774336
9	0.009802052985541971
10-11	0.004084188743975822
12-13	2.7227924959838813E-4
14-15	2.7227924959838813E-4
16-17	5.445584991967763E-4
18-19	0.002178233996787105
20-21	0.0
22-23	0.0013613962479919405
24-25	0.0035396302447790458
26-27	2.7227924959838813E-4
28-29	0.009529773735943584
30-31	2.7227924959838813E-4
32-33	5.445584991967763E-4
34-35	0.002995071745582269
36-37	0.020693222969477496
38-39	0.010346611484738748
40-41	0.002178233996787105
42-43	0.0013613962479919405
44-45	0.005990143491164538
46-47	0.0019059547471887168
48-49	5.445584991967763E-4
50-51	0.002178233996787105
52-53	0.011708007732730688
54-55	0.008168377487951644
56-57	0.015247637977509734
58-59	0.0114357284831323
60-61	0.02586652871184687
62-63	0.010618890734337135
64-65	0.024505132463854933
66-67	0.019059547471887168
68-69	8.168377487951644E-4
70-71	0.0
72-73	0.004084188743975822
74-75	0.008440656737550033
76	0.002178233996787105
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	183635.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.80963460336751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.4641492644581	46.497569770290504
2	9.693867388184303	11.78961067537976
3	5.579881969251761	10.179317510403548
4	3.2348899406458576	7.868499010911352
5	1.8314226197778734	5.5684070156517285
6	0.9976069475379497	3.6398468374538124
7	0.598333105333706	2.5469092254508605
8	0.40518960965278145	1.9711545686453404
9	0.23793638073045506	1.3021941933960037
>10	0.9557272252609683	8.599562413988481
>50	9.955491662690169E-4	0.03692877842860871
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002722792495983881	0.0
2	0.0	0.0	0.0	0.00435646799357421	0.0
3	0.0	0.0	0.0	0.00435646799357421	0.0
4	0.0	0.0	0.0	0.007079260489558091	0.0
5	0.0	0.0	0.0	0.010346611484738748	0.0
6	0.0	0.0	0.0	0.010346611484738748	0.0
7	0.0	0.0	0.0	0.010346611484738748	0.0
8	0.0	0.0	0.0	0.012524845481525854	0.0
9	0.0	0.0	0.0	0.013613962479919405	0.0
10	0.0	0.0	0.0	0.014703079478312958	0.0
11	0.0	0.0	0.0	0.014703079478312958	0.0
12	0.0	0.0	0.0	0.014703079478312958	0.0
13	0.0	0.0	0.0	0.014703079478312958	0.0
14	0.0	0.0	0.0	0.01851498897269039	0.0
15	0.0	0.0	0.0	0.019059547471887168	0.0
16	0.0	0.0	0.0	0.022326898467067823	0.0
17	0.0	0.0	0.0	0.02450513246385493	0.0
18	0.0	0.0	0.0	0.025049690963051707	0.0
19	0.0	0.0	0.0	0.02722792495983881	0.0
20	0.0	0.0	0.0	0.031039834454216243	0.0
21	0.0	0.0	0.0	0.038119094943774336	0.0
22	0.0	0.0	0.0	0.050099381926103415	0.0
23	0.0	0.0	0.0	0.052277615922890514	0.0
24	0.0	0.0	0.0	0.06044599341084216	0.0
25	0.0	0.0	0.0	0.062079668908432487	0.0
26	0.0	0.0	0.0	0.06534701990361315	0.0
27	0.0	0.0	0.0	0.10292155634819071	0.0
28	0.0	0.0	0.0	0.14049609279276826	0.0
29	0.0	0.0	0.0	0.17970430473493615	0.0
30	0.0	0.0	0.0	0.22381354316987503	0.0
31	0.0	0.0	0.0	0.27336836659678165	0.0
32	0.0	0.0	0.0	0.32891333351485286	0.0
33	0.0	0.0	0.0	0.37955727394015304	0.0
34	0.0	0.0	0.0	0.43673591635581455	0.0
35	0.0	0.0	0.0	0.5271326272224793	0.0
36	0.0	0.0	0.0	0.6943120864758897	0.0
37	0.0	0.0	0.0	1.0940180248863234	0.0
38	0.0	0.0	0.0	1.5318430582405316	0.0
39	0.0	0.0	0.0	2.0720450894437334	0.0
40	0.0	0.0	0.0	2.7625452664252457	0.0
41	0.0	0.0	0.0	3.670324284586272	0.0
42	0.0	0.0	0.0	4.550330819288262	0.0
43	0.0	0.0	0.0	5.503852751381817	0.0
44	0.0	0.0	0.0	6.336482696653688	0.0
45	0.0	0.0	0.0	7.144062950962507	0.0
46	0.0	0.0	0.0	7.972880986740001	0.0
47	0.0	0.0	0.0	8.801154464018298	0.0
48	0.0	0.0	0.0	9.578784000871293	0.0
49	0.0	0.0	0.0	10.335175756255616	0.0
50	0.0	0.0	0.0	11.104092357121464	0.0
51	0.0	0.0	0.0	11.971574046341928	0.0
52	0.0	0.0	0.0	12.870095570016609	0.0
53	0.0	0.0	0.0	13.813270890625425	0.0
54	0.0	0.0	0.0	14.665504941868381	0.0
55	0.0	0.0	0.0	15.399569798785635	0.0
56	0.0	0.0	0.0	16.130911863206904	0.0
57	0.0	0.0	0.0	16.75334222778882	0.0
58	0.0	0.0	0.0	17.33275247093419	0.0
59	0.0	0.0	0.0	17.899093310098838	0.0
60	0.0	0.0	0.0	18.51553353118959	0.0
61	0.0	0.0	0.0	19.157568001742586	0.0
62	0.0	0.0	0.0	19.7805429248237	0.0
63	0.0	0.0	0.0	20.438369591853405	0.0
64	0.0	0.0	0.0	21.130503444332508	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAG	15	0.002222467	69.99564	23
CAATTTA	25	2.3864614E-4	55.99651	47
GACGAAT	45	2.6882844E-8	54.44105	23
CTCGGTG	65	3.6379788E-12	53.857475	44
CGCGTTA	20	0.006937006	52.511036	50
TCGTCTC	20	0.0069444887	52.496727	7
TAGATCC	40	7.452818E-7	52.496727	39
CGACGGA	20	0.0069519775	52.482426	22
TCGCTGC	20	0.0069519775	52.482426	15
GGTACGG	20	0.0069594723	52.468136	1
ACGAAGG	55	2.2700988E-9	50.905922	24
TCGGTGG	70	7.2759576E-12	49.98327	45
TGGTCGC	65	1.8371793E-10	48.471725	49
ACGATAT	30	5.868227E-4	46.66376	24
TTCCGAC	60	4.9167284E-9	46.65105	19
GTAGATC	140	0.0	44.9972	38
CGTACCA	40	4.566612E-5	43.771126	56
GATCTCG	105	0.0	43.34244	41
ATCTCGG	115	0.0	42.617653	42
TCTCGGT	85	5.638867E-11	41.185127	43
>>END_MODULE