FastQCFastQC Report
Wed 25 May 2016
SRR1779813_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779813_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183635
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT2163411.780978571623056No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG28201.535654967734909No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC16550.9012443161706647No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT6530.35559669997549487TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC4640.2526751436273042No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT3370.18351621422931358No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT3030.1650012252566232No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2240.12198110382007787No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGCG150.002218146870.0299847
TATACGA207.894139E-570.0299846
TTATACG207.9048055E-570.010945
ATATCGT150.00222535169.9727737
ATCGTAT207.926175E-569.9727639
CGAATGG252.3774778E-456.0392650
CGAATCT252.3903218E-455.9782134
TCTCGTA32050.053.05267738
CGTATCT31800.052.91965534
ATCTCGT32200.052.91419237
GTATCTC31950.052.67120735
ACGTATC31900.052.64409333
ACTATCG200.00692355852.53680454
CGAACTG200.006931028352.52248464
GCCGTCC407.427625E-752.52248446
TATCTCG32050.052.50686636
GACGTGC200.00695347552.47957626
TAGCGTG200.00695347552.47957626
CGCGAAG200.00695347552.4795763
GCAATTG200.00695347552.47957615