##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779813_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	183635
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.58080431290331	34.0	34.0	34.0	34.0	34.0
2	33.67323767255697	34.0	34.0	34.0	34.0	34.0
3	33.776240912680045	34.0	34.0	34.0	34.0	34.0
4	33.5827756146704	34.0	34.0	34.0	34.0	34.0
5	33.61517139978762	34.0	34.0	34.0	34.0	34.0
6	37.30604732213358	38.0	38.0	38.0	37.0	38.0
7	37.3574917635527	38.0	38.0	38.0	37.0	38.0
8	37.26192174694366	38.0	38.0	38.0	36.0	38.0
9	37.179393906390395	38.0	38.0	38.0	36.0	38.0
10-11	37.12503335420807	38.0	38.0	38.0	35.5	38.0
12-13	37.2791842513682	38.0	38.0	38.0	36.5	38.0
14-15	37.29833092819996	38.0	38.0	38.0	36.5	38.0
16-17	37.22079396629183	38.0	38.0	38.0	36.0	38.0
18-19	37.100751490728896	38.0	38.0	38.0	36.0	38.0
20-21	37.092822718980585	38.0	38.0	38.0	36.0	38.0
22-23	37.10563345767419	38.0	38.0	38.0	36.0	38.0
24-25	37.26766956190269	38.0	38.0	38.0	36.0	38.0
26-27	37.174835951752115	38.0	38.0	38.0	36.0	38.0
28-29	36.698518800882184	38.0	37.0	38.0	34.0	38.0
30-31	36.86637895825959	38.0	37.5	38.0	35.0	38.0
32-33	36.96436681460506	38.0	38.0	38.0	36.0	38.0
34-35	37.103046804803	38.0	38.0	38.0	36.0	38.0
36-37	36.987649413238216	38.0	37.5	38.0	35.5	38.0
38-39	36.40492008604024	38.0	37.0	38.0	34.0	38.0
40-41	36.82145015928336	38.0	37.0	38.0	35.0	38.0
42-43	37.16591336074278	38.0	38.0	38.0	36.0	38.0
44-45	36.91713181038473	38.0	37.5	38.0	35.5	38.0
46-47	37.06826585345931	38.0	38.0	38.0	36.0	38.0
48-49	36.711863206905	38.0	37.0	38.0	34.5	38.0
50-51	36.86350368938383	38.0	37.5	38.0	35.0	38.0
52-53	36.78586598415335	38.0	37.0	38.0	35.0	38.0
54-55	36.795488332834154	38.0	37.0	38.0	35.0	38.0
56-57	36.66106679009992	38.0	37.0	38.0	34.5	38.0
58-59	36.750908051297415	38.0	37.0	38.0	34.5	38.0
60-61	36.41310752307567	38.0	37.0	38.0	34.0	38.0
62-63	36.27816592697471	38.0	37.0	38.0	34.0	38.0
64-65	36.65683012497618	38.0	37.0	38.0	34.0	38.0
66-67	36.42319002368829	38.0	37.0	38.0	34.0	38.0
68-69	36.485528357883844	38.0	37.0	38.0	34.0	38.0
70-71	35.58134887140251	38.0	37.0	38.0	29.5	38.0
72-73	33.888003376262695	38.0	35.5	38.0	20.5	38.0
74-75	33.625398208402544	38.0	35.5	38.0	19.0	38.0
76	33.358499196776215	38.0	34.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	3.0
22	7.0
23	13.0
24	36.0
25	91.0
26	193.0
27	395.0
28	609.0
29	1078.0
30	1595.0
31	2643.0
32	4198.0
33	6522.0
34	11200.0
35	21661.0
36	29856.0
37	103534.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.14835841387802	16.717699491124645	11.527061925880725	36.60688016911661
2	17.61537833201732	16.968987393470744	41.91357856617747	23.502055708334467
3	16.65695537343099	19.269202494077923	39.251232063604434	24.82261006888665
4	11.954148174367631	26.903368094317532	34.177580526588066	26.96490320472677
5	12.096822501157186	31.089933836142347	43.5467095052686	13.266534157431861
6	25.253900400250494	45.12320636044327	16.838837912162713	12.784055327143518
7	24.068941105998313	27.166389849429574	19.77509733983173	28.98957170474038
8	21.372287417975876	42.795218776377055	16.922699921039015	18.909793884608053
9	20.726985596427696	16.228387834563126	17.7874588177635	45.257167751245674
10-11	21.602635663136112	29.06962180412231	28.76466904457211	20.56307348816947
12-13	21.566150243689926	19.88564271516868	30.01361396247992	28.534593078661473
14-15	19.705121572684945	20.40161189315762	21.142756010564433	38.75051052359299
16-17	27.005745092166528	29.31957415525363	21.4025104146813	22.272170337898547
18-19	27.22492988809323	24.088272932719796	22.286056579628067	26.400740599558908
20-21	19.80913224603153	22.68739619353609	21.992267269311405	35.511204291120976
22-23	19.917771666621288	29.807770849783537	21.983554333324257	28.290903150270918
24-25	26.87118635760904	29.845044613319466	22.030566318237142	21.25320271083435
26-27	27.203419827374958	31.025675933237128	22.40204753995698	19.368856699430935
28-29	26.734010401067337	23.8004737658943	22.75165409644131	26.713861736597057
30-31	27.33411386718218	29.852969205216873	22.60625697715577	20.20665995044518
32-33	20.55218231818553	29.951534293571484	22.492171971574045	27.004111416668934
34-35	25.838483949138237	23.961935360906146	22.664797015819424	27.534783674136193
36-37	21.022680861491548	28.77746616930324	29.92022217986767	20.279630789337546
38-39	19.05736923789038	23.902850763743295	30.7934217333297	26.246358265036623
40-41	18.879298608653034	21.4022381354317	31.271271816374874	28.447191439540394
42-43	26.478756657263908	21.136064127558075	30.757648365771047	21.627530849406973
44-45	19.042666158412068	28.616276853540988	32.03964385874152	20.301413129305416
46-47	25.813978816674382	21.376916165219047	25.696898739347073	27.112206278759494
48-49	24.666049500367578	21.02485909548833	24.981076592152913	29.328014811991178
50-51	18.80625141266785	21.237810208244262	32.14047432961251	27.815464049475374
52-53	17.009391103509135	21.454368170477945	39.37427142685942	22.161969299153494
54-55	16.629891144454252	21.455929303825744	33.197944929336515	28.716234622383492
56-57	23.653462136752836	21.445714861702506	26.139084809205848	28.76173819233881
58-59	17.20297434221278	21.40273465163153	38.208040529498284	23.186250476657406
60-61	24.200058831219767	28.33159018608097	25.79640040092387	21.671950581775395
62-63	17.053993327432423	38.017294205760194	22.56825764281337	22.360454823994008
64-65	16.885613759898916	38.77923379410259	21.66269076173981	22.672461684258685
66-67	16.630077735590042	38.61830027618742	21.37674251379575	23.374879474426788
68-69	16.743592047685567	38.718382306890064	21.097160160004794	23.440865485419575
70-71	17.45711017918414	37.461194924023744	20.944393006916833	24.13730188987528
72-73	18.633215590046383	34.38176604036679	20.68859397085377	26.29642439873306
74-75	19.137469977288944	32.89372641072703	20.818469481675937	27.150334130308096
76	19.394929608147482	33.120387767883884	20.077880347466166	27.40680227650246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	5.0
12	10.0
13	5.0
14	3.0
15	11.0
16	23.0
17	30.0
18	35.0
19	47.0
20	66.0
21	78.0
22	152.5
23	258.0
24	386.0
25	483.0
26	566.5
27	792.0
28	1087.5
29	1241.0
30	1446.0
31	1769.5
32	2271.0
33	2654.0
34	2912.0
35	3547.5
36	4372.5
37	4820.0
38	5235.5
39	6435.5
40	7681.5
41	11445.5
42	14748.0
43	16504.0
44	17583.5
45	15199.0
46	13491.0
47	12451.5
48	10429.5
49	8880.0
50	8313.0
51	7831.0
52	6803.5
53	5661.0
54	5064.0
55	4841.5
56	4214.0
57	3396.0
58	2983.0
59	2765.0
60	2271.0
61	1823.0
62	1651.0
63	1598.5
64	1449.5
65	1299.0
66	1151.0
67	1057.0
68	1000.0
69	900.5
70	814.0
71	770.0
72	706.0
73	610.0
74	573.5
75	569.0
76	517.5
77	430.0
78	358.5
79	323.0
80	280.0
81	232.5
82	168.5
83	109.0
84	101.5
85	96.0
86	67.5
87	37.0
88	26.0
89	19.0
90	15.0
91	5.5
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05064394042530019
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	8.168377487951644E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	5.445584991967763E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.015519917227108123
52-53	0.031039834454216247
54-55	0.04846570642851308
56-57	0.054183570670079235
58-59	0.035396302447790455
60-61	0.0321289514526098
62-63	0.02586652871184687
64-65	0.015792196476706512
66-67	0.03485174394859368
68-69	0.008440656737550033
70-71	0.013613962479919407
72-73	0.023688294715059764
74-75	0.01306940398072263
76	0.010891169983935524
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	183635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.56192991532115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	41.0931381593784	10.915130557900182
2	16.241261250179388	8.627984861273722
3	12.885171289747216	10.267650502355217
4	9.586485433708509	10.185422168976501
5	6.552268487196835	8.702044817164484
6	4.272505484142116	6.80915947395649
7	2.5503823523381923	4.742015411005527
8	1.5806630174057443	3.3588368230457153
9	1.0660762244500481	2.5485337762409124
>10	4.098243024376242	17.3817627358619
>50	0.05330381122250241	1.0057995480164457
>100	0.01230087951288517	0.8821847686987775
>500	0.002050146585480862	0.35559669997549487
>1k	0.004100293170961724	2.4368992839055736
>5k	0.0	0.0
>10k+	0.002050146585480862	11.780978571623056
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT	21634	11.780978571623056	No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG	2820	1.535654967734909	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	1655	0.9012443161706647	No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT	653	0.35559669997549487	TruSeq Adapter, Index 2 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC	464	0.2526751436273042	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTACCTCGTATGCCGT	337	0.18351621422931358	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTAACTCGTATGCCGT	303	0.1650012252566232	No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC	224	0.12198110382007787	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007079260489558091	0.0
2	0.0	0.0	0.0	0.00871293598714842	0.0
3	0.0	0.0	0.0	0.00871293598714842	0.0
4	0.0	0.0	0.0	0.013069403980722628	0.0
5	0.0	0.0	0.0	0.01742587197429684	0.0
6	0.0	0.0	0.0	0.01742587197429684	0.0
7	0.0	0.0	0.0	0.01742587197429684	0.0
8	0.0	0.0	0.0	0.019604105971083943	0.0
9	0.0	0.0	0.0	0.020693222969477496	0.0
10	0.0	0.0	0.0	0.021237781468674274	0.0
11	0.0	0.0	0.0	0.021237781468674274	0.0
12	0.0	0.0	0.0	0.021237781468674274	0.0
13	0.0	0.0	0.0	0.021237781468674274	0.0
14	0.0	0.0	0.0	0.023960573964658154	0.0
15	0.0	0.0	0.0	0.023960573964658154	0.0
16	0.0	0.0	0.0	0.026138807961445257	0.0
17	0.0	0.0	0.0	0.02886160045742914	0.0
18	0.0	0.0	0.0	0.029406158956625916	0.0
19	0.0	0.0	0.0	0.032673509951806574	0.0
20	0.0	0.0	0.0	0.03866365344297111	0.0
21	0.0	0.0	0.0	0.03866365344297111	0.0
22	0.0	0.0	0.0	0.04247556293734855	0.0
23	0.0	0.0	0.0	0.04519835543333243	0.0
24	0.0	0.0	0.0	0.047376589430119534	0.0
25	0.0	0.0	0.0	0.04955482342690663	0.0
26	0.0	0.0	0.0	0.05336673292128407	0.0
27	0.0	0.0	0.0	0.09420862036104229	0.0
28	0.0	0.0	0.0	0.1328722738040134	0.0
29	0.0	0.0	0.0	0.1764369537397555	0.0
30	0.0	0.0	0.0	0.22109075067389114	0.0
31	0.0	0.0	0.0	0.2722792495983881	0.0
32	0.0	0.0	0.0	0.3300024505132464	0.0
33	0.0	0.0	0.0	0.38336918343453047	0.0
34	0.0	0.0	0.0	0.44381517684537264	0.0
35	0.0	0.0	0.0	0.5374792387072181	0.0
36	0.0	0.0	0.0	0.7215400114357284	0.0
37	0.0	0.0	0.0	1.148473874806001	0.0
38	0.0	0.0	0.0	1.593922727148964	0.0
39	0.0	0.0	0.0	2.1352138753505594	0.0
40	0.0	0.0	0.0	2.854031094290304	0.0
41	0.0	0.0	0.0	3.79557273940153	0.0
42	0.0	0.0	0.0	4.710431018052114	0.0
43	0.0	0.0	0.0	5.698260135595066	0.0
44	0.0	0.0	0.0	6.560296239823563	0.0
45	0.0	0.0	0.0	7.3853023661066795	0.0
46	0.0	0.0	0.0	8.246249353336783	0.0
47	0.0	0.0	0.0	9.101750755574917	0.0
48	0.0	0.0	0.0	9.899528956898195	0.0
49	0.0	0.0	0.0	10.703297301712636	0.0
50	0.0	0.0	0.0	11.489639774552781	0.0
51	0.0	0.0	0.0	12.379448362240314	0.0
52	0.0	0.0	0.0	13.283415470906963	0.0
53	0.0	0.0	0.0	14.225501674517385	0.0
54	0.0	0.0	0.0	15.083181310752307	0.0
55	0.0	0.0	0.0	15.814523375173579	0.0
56	0.0	0.0	0.0	16.551311024586816	0.0
57	0.0	0.0	0.0	17.167751245677568	0.0
58	0.0	0.0	0.0	17.742260462330165	0.0
59	0.0	0.0	0.0	18.330928199961882	0.0
60	0.0	0.0	0.0	18.947912979551827	0.0
61	0.0	0.0	0.0	19.603561412584746	0.0
62	0.0	0.0	0.0	20.230348245160236	0.0
63	0.0	0.0	0.0	20.89035314618673	0.0
64	0.0	0.0	0.0	21.598823753641735	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTGCG	15	0.0022181468	70.02998	47
TATACGA	20	7.894139E-5	70.02998	46
TTATACG	20	7.9048055E-5	70.0109	45
ATATCGT	15	0.002225351	69.97277	37
ATCGTAT	20	7.926175E-5	69.97276	39
CGAATGG	25	2.3774778E-4	56.03926	50
CGAATCT	25	2.3903218E-4	55.97821	34
TCTCGTA	3205	0.0	53.052677	38
CGTATCT	3180	0.0	52.919655	34
ATCTCGT	3220	0.0	52.914192	37
GTATCTC	3195	0.0	52.671207	35
ACGTATC	3190	0.0	52.644093	33
ACTATCG	20	0.006923558	52.536804	54
CGAACTG	20	0.0069310283	52.522484	64
GCCGTCC	40	7.427625E-7	52.522484	46
TATCTCG	3205	0.0	52.506866	36
GACGTGC	20	0.006953475	52.479576	26
TAGCGTG	20	0.006953475	52.479576	26
CGCGAAG	20	0.006953475	52.479576	3
GCAATTG	20	0.006953475	52.479576	15
>>END_MODULE