FastQCFastQC Report
Wed 25 May 2016
SRR1779812_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779812_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112083
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACTTG150.00221763170.0160748
TCGGGCC207.9059624E-569.9848343
GACGATT252.827921E-669.9848323
GTACATC150.002221562469.98482538
GTGGGAT150.002221562469.98482511
TATACTG150.002221562469.9848255
GCCGTAT308.319692E-658.34672554
TCTTGGA200.006929454452.51205448
CTCGAGG200.006929454452.51205465
TGTACAT200.006929454452.51205437
AGATCTC407.434446E-752.4886240
GATGTTC200.006941703652.488628
CCGTAGG200.006941703652.4886243
GTTGTGC200.006941703652.4886223
ACGTATA200.006941703652.4886224
CGACGAT552.2591848E-950.89805622
ATCTCGG506.14491E-848.98938442
TGACGCA506.14491E-848.98938413
TCGGTGG651.8371793E-1048.45103545
TCGCCGT751.4551915E-1146.6773852
Produced by FastQC (version 0.11.5)