##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779812_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	112083
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.796472257166563	34.0	23.0	34.0	12.0	34.0
2	29.56500985876538	34.0	31.0	34.0	12.0	34.0
3	29.131554294585264	34.0	27.0	34.0	11.0	34.0
4	29.4191447409509	34.0	27.0	34.0	12.0	34.0
5	29.591088746732332	34.0	31.0	34.0	11.0	34.0
6	32.41190903169972	37.0	31.0	38.0	12.0	38.0
7	32.416682280096	37.0	31.0	38.0	12.0	38.0
8	31.78577482758313	37.0	27.0	38.0	11.0	38.0
9	32.75382529018674	37.0	33.0	38.0	12.0	38.0
10-11	31.349058287162194	37.0	27.0	38.0	11.5	38.0
12-13	32.20448239251269	37.0	31.0	38.0	11.0	38.0
14-15	31.013936100925207	37.0	26.0	38.0	11.0	38.0
16-17	31.62707547085642	37.0	28.5	38.0	11.0	38.0
18-19	32.371180286038026	37.5	31.0	38.0	11.0	38.0
20-21	31.23926465208819	37.0	27.0	38.0	11.0	38.0
22-23	32.06935485309994	37.0	29.0	38.0	11.0	38.0
24-25	32.35310885682931	37.5	31.0	38.0	11.0	38.0
26-27	31.309458169392325	37.0	26.5	38.0	11.0	38.0
28-29	32.43879535701221	37.5	32.0	38.0	11.0	38.0
30-31	32.78349080592061	38.0	34.0	38.0	11.0	38.0
32-33	32.89185246647574	38.0	34.0	38.0	11.0	38.0
34-35	32.8544917605703	38.0	34.0	38.0	11.0	38.0
36-37	32.35657949912118	38.0	32.0	38.0	11.0	38.0
38-39	32.30314142198192	38.0	32.0	38.0	11.0	38.0
40-41	32.335818099087284	37.5	31.0	38.0	11.0	38.0
42-43	32.68144143179608	38.0	32.5	38.0	11.0	38.0
44-45	32.754811166724664	38.0	34.0	38.0	11.0	38.0
46-47	32.63558702033315	38.0	33.0	38.0	11.0	38.0
48-49	32.38278329452281	37.5	32.0	38.0	11.0	38.0
50-51	31.793867044957754	37.5	28.5	38.0	11.0	38.0
52-53	32.34446793893811	37.0	31.0	38.0	11.0	38.0
54-55	32.458521809730286	37.5	32.0	38.0	11.0	38.0
56-57	32.53148559549619	38.0	32.0	38.0	11.0	38.0
58-59	32.725966471275754	38.0	34.0	38.0	11.0	38.0
60-61	32.64296101995842	38.0	32.5	38.0	11.0	38.0
62-63	32.617082876082904	38.0	33.0	38.0	11.0	38.0
64-65	32.62412230222246	38.0	32.5	38.0	11.0	38.0
66-67	32.52885361740853	38.0	32.0	38.0	11.0	38.0
68-69	31.95515823095385	37.0	29.0	38.0	11.0	38.0
70-71	32.04126406323885	37.0	30.5	38.0	11.0	38.0
72-73	32.20345636715648	37.0	31.0	38.0	11.0	38.0
74-75	32.22214341157892	37.5	31.0	38.0	11.0	38.0
76	31.35033858836755	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	15.0
13	223.0
14	1051.0
15	2067.0
16	2866.0
17	3245.0
18	3278.0
19	2818.0
20	2227.0
21	1778.0
22	1312.0
23	1092.0
24	985.0
25	972.0
26	1022.0
27	1178.0
28	1465.0
29	1782.0
30	2057.0
31	2625.0
32	3397.0
33	4521.0
34	6289.0
35	9535.0
36	17494.0
37	36789.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.34954453396144	21.307423962599145	10.90709563448516	33.43593586895426
2	17.354995851288777	20.77478297333226	36.65676329148934	25.213457883889618
3	16.126442011723455	26.04498452040006	31.4650749890706	26.363498478805887
4	12.149924609441218	22.640364729709233	32.00396135007093	33.20574931077862
5	11.996466903990791	33.8864948297244	33.5688730672805	20.54816519900431
6	25.164836145287783	42.42021395240942	15.890293626930523	16.524656275372276
7	23.963881974000017	28.63656236337518	23.539173960759122	23.86038170186568
8	20.571545832515483	37.85710461983191	20.822255134633572	20.74909441301904
9	22.982269851609278	17.35716389010342	20.620332116820887	39.04023414146642
10-11	22.4330377058834	26.92142971858884	25.590660076018484	25.054872499509273
12-13	21.915107175518035	23.218165190819263	26.589342671692727	28.277384961969975
14-15	23.96482963518107	25.32988945692032	23.015979229678006	27.689301678220605
16-17	24.94825217251655	26.12774575757035	23.385557002908584	25.538445067004517
18-19	21.687633832976445	26.204496788008562	25.704853675945756	26.403015703069237
20-21	23.792635814530303	25.345056788272974	24.94892178118002	25.913385616016704
22-23	25.403389497820783	25.133942711330594	23.826412030531355	25.636255760317265
24-25	21.595289079229122	28.717880085653103	23.790149892933616	25.89668094218415
26-27	23.03313645366785	28.700861868988774	24.544083795792364	23.721917881551008
28-29	22.216967663644798	27.87493754015276	26.03951031479763	23.868584481404813
30-31	22.768394850244906	26.898369957977568	26.137326802458894	24.195908389318628
32-33	21.08705156000464	28.25584611404049	25.919630987750146	24.737471338204724
34-35	21.029973991907603	29.362377933717283	25.479900607152896	24.127747467222218
36-37	23.587915348542516	26.277111712973912	25.45344214457498	24.681530793908593
38-39	23.682860342373775	25.5138506565064	26.733172422716056	24.070116578403773
40-41	21.815375418780082	26.907205917122806	26.485191579341816	24.79222708475529
42-43	20.106440877579608	26.62360257313907	27.164282973920646	26.105673575360676
44-45	19.8163754221447	25.513377023729326	28.538988994124548	26.13125856000143
46-47	21.077543518411122	25.92858608659963	27.42217542983074	25.571694965158503
48-49	20.868017451353015	26.419706824406912	27.309226198442225	25.403049525797844
50-51	23.180899613227872	26.414261051110127	25.469412882589904	24.935426453072097
52-53	18.592812367546344	27.790840341758315	26.886474669284137	26.729872621411204
54-55	19.427156528140266	26.501594949697736	26.555577862544332	27.51567065961766
56-57	20.142238244598026	26.878031151424835	26.160147413789257	26.819583190187878
58-59	19.861244339349053	27.173801503558124	26.01958640997613	26.945367747116695
60-61	18.822815479829192	26.237444837602798	26.360151889019278	28.579587793548733
62-63	19.64861717742691	26.506292857684606	26.106101908156848	27.73898805673164
64-65	20.597369219511325	26.103446429527565	25.340894893715753	27.958289457245357
66-67	19.608779264587117	28.698146966566867	24.11843708030109	27.574636688544924
68-69	20.34885017732474	29.76691276515067	23.159279994646802	26.72495706287779
70-71	21.006495244553093	29.84065237950786	23.000571010510164	26.15228136542888
72-73	21.092453469904886	30.018826174628384	22.81982190974143	26.068898445725296
74-75	22.218553978210632	29.732852694226086	22.27342892578944	25.775164401773843
76	21.376885946519867	29.76293507258273	23.214875221941668	25.645303758955734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	0.5
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	3.0
15	4.5
16	13.0
17	23.0
18	26.0
19	32.0
20	44.5
21	54.0
22	76.5
23	123.0
24	200.0
25	253.0
26	302.5
27	414.5
28	543.0
29	609.0
30	721.0
31	1066.5
32	1503.5
33	1707.0
34	1919.5
35	2407.0
36	2972.0
37	3262.0
38	3616.0
39	4524.5
40	5287.0
41	5753.5
42	6012.0
43	6358.0
44	6869.5
45	7170.0
46	7305.0
47	7321.5
48	6994.5
49	6434.0
50	6217.0
51	5914.5
52	5215.0
53	4542.0
54	4266.0
55	4019.0
56	3454.0
57	2829.0
58	2522.0
59	2236.5
60	1815.0
61	1565.0
62	1451.0
63	1262.5
64	1004.5
65	874.0
66	759.5
67	706.0
68	670.0
69	551.5
70	448.5
71	428.0
72	407.5
73	357.0
74	292.0
75	257.0
76	219.5
77	176.0
78	151.5
79	133.0
80	131.0
81	113.0
82	84.5
83	72.0
84	72.0
85	61.5
86	45.5
87	40.0
88	30.0
89	22.0
90	16.0
91	8.0
92	8.0
93	8.0
94	6.0
95	4.0
96	4.0
97	3.5
98	4.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.001784391923842153
7	0.005353175771526458
8	8.921959619210765E-4
9	0.012490743466895068
10-11	0.004460979809605381
12-13	4.4609798096053823E-4
14-15	0.0
16-17	8.921959619210765E-4
18-19	0.002676587885763229
20-21	0.0
22-23	0.0013382939428816145
24-25	0.002676587885763229
26-27	8.921959619210765E-4
28-29	0.009814155581131839
30-31	0.0
32-33	0.0
34-35	0.0022304899048026907
36-37	0.020966605105145295
38-39	0.011152449524013454
40-41	0.0013382939428816145
42-43	0.001784391923842153
44-45	0.005799273752486996
46-47	0.003568783847684306
48-49	0.0
50-51	0.0013382939428816145
52-53	0.013829037409776682
54-55	0.0093680576001713
56-57	0.015613429333618837
58-59	0.013829037409776682
60-61	0.024535388952829598
62-63	0.010260253562092378
64-65	0.02230489904802691
66-67	0.020966605105145295
68-69	4.4609798096053823E-4
70-71	8.921959619210765E-4
72-73	0.004460979809605381
74-75	0.008921959619210763
76	0.003568783847684306
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	112083.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.36793269273663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.15034168564921	54.669307566713954
2	8.309053345340892	11.195274930185665
3	4.345234941992902	8.781884853189155
4	2.4871536790803623	6.702176065951125
5	1.338930974201409	4.51005058751104
6	0.7151560099592096	2.8907149166242876
7	0.4595539545478625	2.167143991506294
8	0.3046034857233671	1.6416405699347805
9	0.19865444721089157	1.204464548593453
>10	0.6899931133124967	6.1900555838084275
>50	0.0013243629814059439	0.047286385981817045
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004460979809605381	0.0
2	0.0	0.0	0.0	0.004460979809605381	0.0
3	0.0	0.0	0.0	0.006245371733447534	0.0
4	0.0	0.0	0.0	0.008029763657289687	0.0
5	0.0	0.0	0.0	0.00981415558113184	0.0
6	0.0	0.0	0.0	0.00981415558113184	0.0
7	0.0	0.0	0.0	0.00981415558113184	0.0
8	0.0	0.0	0.0	0.00981415558113184	0.0
9	0.0	0.0	0.0	0.013382939428816146	0.0
10	0.0	0.0	0.0	0.013382939428816146	0.0
11	0.0	0.0	0.0	0.014275135390737222	0.0
12	0.0	0.0	0.0	0.014275135390737222	0.0
13	0.0	0.0	0.0	0.01695172327650045	0.0
14	0.0	0.0	0.0	0.017843919238421525	0.0
15	0.0	0.0	0.0	0.017843919238421525	0.0
16	0.0	0.0	0.0	0.017843919238421525	0.0
17	0.0	0.0	0.0	0.017843919238421525	0.0
18	0.0	0.0	0.0	0.020520507124184757	0.0
19	0.0	0.0	0.0	0.02676587885763229	0.0
20	0.0	0.0	0.0	0.028550270781474443	0.0
21	0.0	0.0	0.0	0.03211905462915875	0.0
22	0.0	0.0	0.0	0.03747223040068521	0.0
23	0.0	0.0	0.0	0.042825406172211665	0.0
24	0.0	0.0	0.0	0.04639419001989597	0.0
25	0.0	0.0	0.0	0.047286385981817045	0.0
26	0.0	0.0	0.0	0.04996297386758027	0.0
27	0.0	0.0	0.0	0.05888493348679104	0.0
28	0.0	0.0	0.0	0.07048348099176503	0.0
29	0.0	0.0	0.0	0.08832740023018656	0.0
30	0.0	0.0	0.0	0.11420108312589777	0.0
31	0.0	0.0	0.0	0.14096696198353006	0.0
32	0.0	0.0	0.0	0.17843919238421527	0.0
33	0.0	0.0	0.0	0.20609726720376864	0.0
34	0.0	0.0	0.0	0.24267730164253276	0.0
35	0.0	0.0	0.0	0.33814226956808796	0.0
36	0.0	0.0	0.0	0.4942765629042763	0.0
37	0.0	0.0	0.0	0.7423070403183355	0.0
38	0.0	0.0	0.0	1.0822337018102657	0.0
39	0.0	0.0	0.0	1.481937492750908	0.0
40	0.0	0.0	0.0	1.963723312188289	0.0
41	0.0	0.0	0.0	2.4597842670164076	0.0
42	0.0	0.0	0.0	3.015622351293238	0.0
43	0.0	0.0	0.0	3.6285609771330174	0.0
44	0.0	0.0	0.0	4.1701239260191105	0.0
45	0.0	0.0	0.0	4.742021537610521	0.0
46	0.0	0.0	0.0	5.385294826155617	0.0
47	0.0	0.0	0.0	6.0026944318050015	0.0
48	0.0	0.0	0.0	6.656674071893151	0.0
49	0.0	0.0	0.0	7.2544453663802715	0.0
50	8.921959619210764E-4	0.0	0.0	7.795116119304444	0.0
51	8.921959619210764E-4	0.0	0.0	8.431251840154172	0.0
52	8.921959619210764E-4	0.0	0.0	9.020101175022083	0.0
53	8.921959619210764E-4	0.0	0.0	9.63214760489994	0.0
54	8.921959619210764E-4	0.0	0.0	10.196907648795982	0.0
55	8.921959619210764E-4	0.0	0.0	10.785756983663893	0.0
56	8.921959619210764E-4	0.0	0.0	11.329104324473828	0.0
57	8.921959619210764E-4	0.0	0.0	11.783232069091655	0.0
58	8.921959619210764E-4	0.0	0.0	12.254311536985984	0.0
59	8.921959619210764E-4	0.0	0.0	12.779814958557498	0.0
60	8.921959619210764E-4	0.0	0.0	13.34546719841546	0.0
61	8.921959619210764E-4	0.0	0.0	13.897736498844607	0.0
62	8.921959619210764E-4	0.0	0.0	14.393797453672725	0.0
63	8.921959619210764E-4	0.0	0.0	14.905025739853501	0.0
64	8.921959619210764E-4	0.0	0.0	15.420715005843883	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACTTG	15	0.002217631	70.01607	48
TCGGGCC	20	7.9059624E-5	69.98483	43
GACGATT	25	2.827921E-6	69.98483	23
GTACATC	15	0.0022215624	69.984825	38
GTGGGAT	15	0.0022215624	69.984825	11
TATACTG	15	0.0022215624	69.984825	5
GCCGTAT	30	8.319692E-6	58.346725	54
TCTTGGA	20	0.0069294544	52.512054	48
CTCGAGG	20	0.0069294544	52.512054	65
TGTACAT	20	0.0069294544	52.512054	37
AGATCTC	40	7.434446E-7	52.48862	40
GATGTTC	20	0.0069417036	52.48862	8
CCGTAGG	20	0.0069417036	52.48862	43
GTTGTGC	20	0.0069417036	52.48862	23
ACGTATA	20	0.0069417036	52.48862	24
CGACGAT	55	2.2591848E-9	50.898056	22
ATCTCGG	50	6.14491E-8	48.989384	42
TGACGCA	50	6.14491E-8	48.989384	13
TCGGTGG	65	1.8371793E-10	48.451035	45
TCGCCGT	75	1.4551915E-11	46.67738	52
>>END_MODULE