FastQCFastQC Report
Wed 25 May 2016
SRR1779812_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779812_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112083
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT1409512.57550208327757No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG19621.7504884772891516No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC10650.9501886994459464No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT4490.40059598690256326RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC3480.31048419474853456No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT2330.20788165912761078No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT2140.19092993585111034No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT1380.12312304274510855No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC1290.11509327908781886No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTG150.00221718370.0196467
GGCACAG150.002221113369.98839628
ACATCCG150.002221113369.98839610
TCTCGTA19950.052.97367538
GCCGTCT20650.052.89619446
CGTCTTC20700.052.7684348
CGTATGC21050.052.69910441
ATCTCGT20000.052.66626737
CTCGTAT21000.052.65793639
TATGCCG21000.052.65793643
TCGTATG21100.052.57422340
AGCGTGG200.006928057452.51473268
GGACTAG200.006928057452.5147321
TCGTGTA200.006928057452.51473259
GCCGAGT200.006928057452.51473258
CCGAGTT200.006928057452.51473259
ATCCCAC200.006928057452.51473246
GAAACGG200.006928057452.51473267
CGTGTAG200.006928057452.51473260
GGGTGTC200.006940304352.491315