##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779812_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	112083
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.59539805322841	34.0	34.0	34.0	34.0	34.0
2	33.67019976267587	34.0	34.0	34.0	34.0	34.0
3	33.77519338347474	34.0	34.0	34.0	34.0	34.0
4	33.5979943434776	34.0	34.0	34.0	34.0	34.0
5	33.619915598262004	34.0	34.0	34.0	34.0	34.0
6	37.31192063024723	38.0	38.0	38.0	37.0	38.0
7	37.361723008841665	38.0	38.0	38.0	37.0	38.0
8	37.27006771767351	38.0	38.0	38.0	36.0	38.0
9	37.18571058947387	38.0	38.0	38.0	36.0	38.0
10-11	37.142608602553466	38.0	38.0	38.0	36.0	38.0
12-13	37.281795633592964	38.0	38.0	38.0	36.5	38.0
14-15	37.29744921174487	38.0	38.0	38.0	36.5	38.0
16-17	37.22292854402541	38.0	38.0	38.0	36.0	38.0
18-19	37.0851734875048	38.0	38.0	38.0	36.0	38.0
20-21	37.10854456072732	38.0	38.0	38.0	36.0	38.0
22-23	37.093792100496955	38.0	38.0	38.0	36.0	38.0
24-25	37.28211236315944	38.0	38.0	38.0	36.0	38.0
26-27	37.19507864707404	38.0	38.0	38.0	36.0	38.0
28-29	36.649728326329594	38.0	37.0	38.0	34.0	38.0
30-31	36.924257023812714	38.0	37.5	38.0	36.0	38.0
32-33	37.02904543954034	38.0	38.0	38.0	36.0	38.0
34-35	37.13473943416932	38.0	38.0	38.0	36.0	38.0
36-37	37.067311724347135	38.0	38.0	38.0	36.0	38.0
38-39	36.23189065246291	38.0	37.0	38.0	34.0	38.0
40-41	36.76554874512638	38.0	37.0	38.0	35.0	38.0
42-43	37.189163387846506	38.0	38.0	38.0	36.0	38.0
44-45	36.98093823327356	38.0	38.0	38.0	35.5	38.0
46-47	37.11246130100015	38.0	38.0	38.0	36.0	38.0
48-49	36.75044832847087	38.0	37.0	38.0	34.5	38.0
50-51	36.897040585994304	38.0	37.5	38.0	35.5	38.0
52-53	36.83027756216376	38.0	37.5	38.0	35.0	38.0
54-55	36.830897638357285	38.0	37.5	38.0	35.0	38.0
56-57	36.71592480572433	38.0	37.0	38.0	34.5	38.0
58-59	36.7844142287412	38.0	37.0	38.0	35.0	38.0
60-61	36.46829135551332	38.0	37.0	38.0	34.0	38.0
62-63	36.30408714970156	38.0	37.0	38.0	34.0	38.0
64-65	36.68367192170088	38.0	37.0	38.0	34.5	38.0
66-67	36.42241017817153	38.0	37.0	38.0	34.0	38.0
68-69	36.47349285797132	38.0	37.0	38.0	34.0	38.0
70-71	35.45283406047304	38.0	36.5	38.0	28.5	38.0
72-73	33.62588885022707	38.0	35.0	38.0	20.0	38.0
74-75	33.34304934735866	38.0	34.5	38.0	19.0	38.0
76	33.1361223379103	38.0	34.0	38.0	16.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	3.0
22	5.0
23	8.0
24	17.0
25	42.0
26	111.0
27	184.0
28	371.0
29	635.0
30	961.0
31	1583.0
32	2570.0
33	4114.0
34	6997.0
35	13896.0
36	17984.0
37	62602.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.683758547428184	17.642962989412794	12.693935119887165	36.97934334327186
2	16.11305907229464	16.54488191786444	45.00593310314677	22.336125906694146
3	15.585771258799284	20.110096981701062	40.94465708448203	23.359474675017623
4	11.238992532319799	28.297779324250776	35.32649911226502	25.136729031164407
5	11.16583246344227	31.84068948903937	44.79715924805724	12.196318799461112
6	24.11427245880285	46.53248039399374	17.00168625036803	12.351560896835382
7	21.939098703639267	27.840082795785264	20.450915839154913	29.769902661420556
8	19.468608085079808	44.79894363998109	17.379977338222567	18.352470936716543
9	19.76214055655184	15.979229678006476	18.19276785953267	46.06586190590901
10-11	21.28378076960824	30.16469937457063	29.223432634743894	19.328087221077237
12-13	20.5075702827369	19.633664338035206	30.62730298082671	29.231462398401188
14-15	19.133588501378444	20.44110168357378	21.158873334939283	39.26643648010849
16-17	27.603650865876183	29.575403941721763	20.404075551154055	22.416869641248006
18-19	27.92617970611065	23.96482963518107	21.34177350713311	26.767217151575174
20-21	19.527493018566595	22.27233389541679	21.32616007779949	36.874013008217126
22-23	19.25626544614259	29.74804386035349	21.581774220889876	29.413916472614044
24-25	26.95737514777062	30.20498293667611	21.375326210603795	21.46231570494948
26-27	27.635323822524377	31.66135810069324	21.5871273966614	19.11619068012098
28-29	19.332994298867803	31.130055405369234	22.92096035973341	26.61598993602955
30-31	19.275447659323895	31.924109811478996	29.264919746973224	19.53552278222389
32-33	34.02567739978409	23.342969049722083	23.894792252170266	18.736561298323565
34-35	21.993076559335492	28.85495570247049	30.714292087114014	18.437675651080003
36-37	27.319932549985275	30.726782830580905	22.767948752263948	19.185335867169865
38-39	19.37894239090674	23.977320378647963	30.28113094760133	26.362606282843963
40-41	18.43499906319424	21.40824210629623	32.03608040470009	28.12067842580944
42-43	26.870265785177054	21.172256274368102	30.727228928561868	21.230249011892973
44-45	18.864591418859238	29.4562957808053	31.81481580614366	19.864296994191804
46-47	26.3768814182347	21.54564028443207	25.152342460498023	26.925135836835203
48-49	25.542232095857536	20.7912885986278	23.773453601349	29.893025704165666
50-51	19.381262994458673	21.189110085930736	31.624385412298	27.80524150731259
52-53	17.302543507362785	21.063364569388664	39.900490852298084	21.733601070950467
54-55	16.693966998585143	21.338891591646544	33.608865838581394	28.35827557118692
56-57	25.01216327805923	21.12259245207222	25.642421942999977	28.222822326868567
58-59	17.648660106655957	21.710511636209475	38.29216591918245	22.348662337952117
60-61	25.098506450331776	28.854023034668025	25.247549052419267	20.799921462580937
62-63	17.33108439756187	39.17967390431314	22.319795096963045	21.16944660116195
64-65	16.303038415205464	40.49703297193593	21.83152634631687	21.36840226654174
66-67	16.697681683289822	40.158868286587676	21.41285672847357	21.73059330164893
68-69	16.87873082722868	40.603445968252835	21.072872145833518	21.444951058684964
70-71	17.335070335190792	39.26010859235927	21.186217604097422	22.21860346835251
72-73	17.997822556176267	35.496796301915076	21.231415873922433	25.27396526798622
74-75	18.376506911044284	34.11753683064595	21.777151168497415	25.728805089812347
76	18.495350953902165	34.25035247086538	22.062891510359968	25.191405064872484
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	2.5
15	2.5
16	9.5
17	18.0
18	23.0
19	40.0
20	52.5
21	53.0
22	75.5
23	127.0
24	193.5
25	231.0
26	288.5
27	397.5
28	505.0
29	561.0
30	661.5
31	864.5
32	1249.5
33	1532.0
34	1742.5
35	2248.5
36	2825.5
37	3107.0
38	3365.0
39	6223.5
40	10233.5
41	11140.5
42	10638.0
43	9651.0
44	8026.0
45	6971.0
46	6554.0
47	6281.5
48	5706.0
49	5145.5
50	4888.0
51	4587.0
52	3742.5
53	3051.0
54	2903.0
55	2749.5
56	2365.0
57	1921.5
58	1709.0
59	1515.5
60	1215.0
61	1000.5
62	893.0
63	834.5
64	744.0
65	645.5
66	510.5
67	442.0
68	487.0
69	452.5
70	410.5
71	448.0
72	386.5
73	290.5
74	244.5
75	233.0
76	217.0
77	169.5
78	122.0
79	106.0
80	90.0
81	75.0
82	62.5
83	49.0
84	39.5
85	28.0
86	20.5
87	15.0
88	16.0
89	14.5
90	11.5
91	7.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05442395367718565
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	4.4609798096053823E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.014275135390737223
52-53	0.029442466743395523
54-55	0.05130126781046189
56-57	0.058438835505830504
58-59	0.03613393645780359
60-61	0.030780760686277137
62-63	0.026765878857632288
64-65	0.016059527314579373
66-67	0.03613393645780359
68-69	0.008921959619210763
70-71	0.011152449524013454
72-73	0.02230489904802691
74-75	0.014275135390737223
76	0.0151673313526583
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	112083.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.875922307575635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	44.143364745867444	12.746803707966418
2	17.7908234203615	10.274528697483115
3	12.918275915340645	11.19081395037606
4	8.638961841495442	9.978319638125317
5	5.379267727483392	7.76656584852297
6	3.176270662752974	5.503064693129199
7	2.0948555538390234	4.234362035277428
8	1.3316854626911787	3.076291676703871
9	0.9083886914877182	2.360750515243168
>10	3.556310829599876	15.51707216973136
>50	0.027807817086358717	0.5272878134953561
>100	0.024718059632318864	1.5479599939330675
>500	0.0	0.0
>1k	0.006179514908079716	2.700677176735098
>5k	0.0	0.0
>10k+	0.003089757454039858	12.57550208327757
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT	14095	12.57550208327757	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG	1962	1.7504884772891516	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	1065	0.9501886994459464	No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT	449	0.40059598690256326	RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC	348	0.31048419474853456	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGACCTCGTATGCCGT	233	0.20788165912761078	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGAACTCGTATGCCGT	214	0.19092993585111034	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATATCGTATGCCGT	138	0.12312304274510855	No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC	129	0.11509327908781886	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008921959619210763	0.0
2	0.0	0.0	0.0	0.008921959619210763	0.0
3	0.0	0.0	0.0	0.010706351543052916	0.0
4	0.0	0.0	0.0	0.012490743466895068	0.0
5	0.0	0.0	0.0	0.015167331352658298	0.0
6	0.0	0.0	0.0	0.015167331352658298	0.0
7	0.0	0.0	0.0	0.015167331352658298	0.0
8	0.0	0.0	0.0	0.015167331352658298	0.0
9	0.0	0.0	0.0	0.020520507124184757	0.0
10	0.0	0.0	0.0	0.020520507124184757	0.0
11	0.0	0.0	0.0	0.02230489904802691	0.0
12	0.0	0.0	0.0	0.02230489904802691	0.0
13	0.0	0.0	0.0	0.024981486933790136	0.0
14	0.0	0.0	0.0	0.024981486933790136	0.0
15	0.0	0.0	0.0	0.024981486933790136	0.0
16	0.0	0.0	0.0	0.024981486933790136	0.0
17	0.0	0.0	0.0	0.024981486933790136	0.0
18	0.0	0.0	0.0	0.030334662705316595	0.0
19	0.0	0.0	0.0	0.036580034438764134	0.0
20	0.0	0.0	0.0	0.03747223040068521	0.0
21	0.0	0.0	0.0	0.03747223040068521	0.0
22	0.0	0.0	0.0	0.04014881828644844	0.0
23	0.0	0.0	0.0	0.04639419001989597	0.0
24	0.0	0.0	0.0	0.047286385981817045	0.0
25	0.0	0.0	0.0	0.05085516982950135	0.0
26	0.0	0.0	0.0	0.05353175771526458	0.0
27	0.0	0.0	0.0	0.06602250118215965	0.0
28	0.0	0.0	0.0	0.07762104868713364	0.0
29	0.0	0.0	0.0	0.09903375177323948	0.0
30	0.0	0.0	0.0	0.12579963063087177	0.0
31	0.0	0.0	0.0	0.1543499014123462	0.0
32	0.0	0.0	0.0	0.19539091566071573	0.0
33	0.0	0.0	0.0	0.22572557836603233	0.0
34	0.0	0.0	0.0	0.2640900047286386	0.0
35	0.0	0.0	0.0	0.36401595246379914	0.0
36	0.0	0.0	0.0	0.5353175771526458	0.0
37	0.0	0.0	0.0	0.7878090343763104	0.0
38	0.0	0.0	0.0	1.1366576554874512	0.0
39	0.0	0.0	0.0	1.5497443858569095	0.0
40	0.0	0.0	0.0	2.057403888190002	0.0
41	0.0	0.0	0.0	2.5623868026373313	0.0
42	0.0	0.0	0.0	3.142314177886031	0.0
43	0.0	0.0	0.0	3.767743547192705	0.0
44	0.0	0.0	0.0	4.317336259736089	0.0
45	0.0	0.0	0.0	4.912430966337446	0.0
46	0.0	0.0	0.0	5.576224762006727	0.0
47	0.0	0.0	0.0	6.21682146266606	0.0
48	0.0	0.0	0.0	6.900243569497604	0.0
49	0.0	0.0	0.0	7.522996350918516	0.0
50	0.0	0.0	0.0	8.079726631157268	0.0
51	0.0	0.0	0.0	8.719431135854679	0.0
52	0.0	0.0	0.0	9.310957058608352	0.0
53	0.0	0.0	0.0	9.915865920790843	0.0
54	0.0	0.0	0.0	10.47794937680112	0.0
55	0.0	0.0	0.0	11.055200164164058	0.0
56	0.0	0.0	0.0	11.58694895746902	0.0
57	0.0	0.0	0.0	12.036615722277242	0.0
58	0.0	0.0	0.0	12.504126406323884	0.0
59	0.0	0.0	0.0	13.036767395590768	0.0
60	0.0	0.0	0.0	13.614018182953703	0.0
61	0.0	0.0	0.0	14.17431724704014	0.0
62	0.0	0.0	0.0	14.67127039783018	0.0
63	0.0	0.0	0.0	15.190528447668246	0.0
64	0.0	0.0	0.0	15.728522612706655	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGTG	15	0.002217183	70.01964	67
GGCACAG	15	0.0022211133	69.988396	28
ACATCCG	15	0.0022211133	69.988396	10
TCTCGTA	1995	0.0	52.973675	38
GCCGTCT	2065	0.0	52.896194	46
CGTCTTC	2070	0.0	52.76843	48
CGTATGC	2105	0.0	52.699104	41
ATCTCGT	2000	0.0	52.666267	37
CTCGTAT	2100	0.0	52.657936	39
TATGCCG	2100	0.0	52.657936	43
TCGTATG	2110	0.0	52.574223	40
AGCGTGG	20	0.0069280574	52.514732	68
GGACTAG	20	0.0069280574	52.514732	1
TCGTGTA	20	0.0069280574	52.514732	59
GCCGAGT	20	0.0069280574	52.514732	58
CCGAGTT	20	0.0069280574	52.514732	59
ATCCCAC	20	0.0069280574	52.514732	46
GAAACGG	20	0.0069280574	52.514732	67
CGTGTAG	20	0.0069280574	52.514732	60
GGGTGTC	20	0.0069403043	52.4913	15
>>END_MODULE