FastQCFastQC Report
Wed 25 May 2016
SRR1779811_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779811_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141312
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGATC308.342275E-658.33333238
CTTACGC308.342275E-658.33333212
CTCGGTG750.056.044
GTGTACA252.3838425E-455.99999636
GCGGAGA252.3838425E-455.99999636
GAGATCT252.3838425E-455.99999639
TCCTCAG200.00693948952.516
GCGTTGA200.00693948952.536
ACGATAT200.00693948952.525
TCTAAGA200.00693948952.517
TCTCGGT800.052.543
TGTTCGA200.00693948952.537
AACGACT200.00693948952.56
CAATGTA200.00693948952.545
GACGACT200.00693948952.523
ACGACTC200.00693948952.524
CTGTGTA200.00693948952.534
GACTATC200.00693948952.538
TCGGTGG750.051.33333245
TCGCCGT552.2628228E-950.9090952
Produced by FastQC (version 0.11.5)