##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779811_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	141312
Sequences flagged as poor quality	0
Sequence length	76
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.127597090126812	34.0	23.0	34.0	12.0	34.0
2	29.79266445878623	34.0	31.0	34.0	12.0	34.0
3	29.32858497509058	34.0	27.0	34.0	11.0	34.0
4	29.61322463768116	34.0	30.0	34.0	12.0	34.0
5	30.097451030344203	34.0	32.0	34.0	11.0	34.0
6	32.87157495471015	38.0	33.0	38.0	12.0	38.0
7	33.01230610280797	38.0	34.0	38.0	12.0	38.0
8	32.396434839221016	37.0	31.0	38.0	11.0	38.0
9	33.05751103940217	38.0	34.0	38.0	12.0	38.0
10-11	31.741448001585148	37.0	28.5	38.0	11.5	38.0
12-13	32.54885289288949	37.0	31.5	38.0	11.0	38.0
14-15	31.33788001019022	37.0	27.0	38.0	11.0	38.0
16-17	31.94941689311594	37.0	29.0	38.0	11.0	38.0
18-19	32.71829710144928	37.5	33.0	38.0	11.0	38.0
20-21	31.533599411231883	37.0	27.5	38.0	11.0	38.0
22-23	32.38815882586051	37.5	31.0	38.0	11.0	38.0
24-25	32.68202983469203	38.0	32.5	38.0	11.0	38.0
26-27	31.626383463541664	37.0	27.0	38.0	11.0	38.0
28-29	32.7439354053442	37.5	32.5	38.0	11.0	38.0
30-31	33.07242130887681	38.0	34.0	38.0	11.0	38.0
32-33	33.184874603713766	38.0	34.0	38.0	11.0	38.0
34-35	33.16407311480978	38.0	34.0	38.0	11.0	38.0
36-37	32.6956592504529	38.0	32.5	38.0	11.0	38.0
38-39	32.62693543365036	38.0	32.5	38.0	11.0	38.0
40-41	32.63195623867753	38.0	32.5	38.0	11.0	38.0
42-43	33.01006637794384	38.0	34.0	38.0	11.0	38.0
44-45	33.097351958786234	38.0	34.0	38.0	11.0	38.0
46-47	32.999805395153984	38.0	34.0	38.0	11.0	38.0
48-49	32.73575138700181	38.0	33.0	38.0	11.0	38.0
50-51	32.150390625	37.5	30.5	38.0	11.0	38.0
52-53	32.68303470335145	37.5	33.0	38.0	11.0	38.0
54-55	32.822424847146735	38.0	33.5	38.0	11.0	38.0
56-57	32.89434372169384	38.0	34.0	38.0	11.0	38.0
58-59	33.07883619225544	38.0	34.0	38.0	11.0	38.0
60-61	33.00316675158514	38.0	34.0	38.0	11.0	38.0
62-63	32.994469684103265	38.0	34.0	38.0	11.0	38.0
64-65	33.003386124320656	38.0	34.0	38.0	11.0	38.0
66-67	32.931102807971016	38.0	34.0	38.0	11.0	38.0
68-69	32.36483101222826	38.0	31.0	38.0	11.0	38.0
70-71	32.46442623414855	37.0	31.5	38.0	11.0	38.0
72-73	32.61011803668478	37.5	32.0	38.0	11.0	38.0
74-75	32.620916836503625	38.0	32.0	38.0	11.0	38.0
76	31.77481742527174	37.0	30.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	25.0
13	326.0
14	1388.0
15	2607.0
16	3533.0
17	4062.0
18	3984.0
19	3481.0
20	2634.0
21	1956.0
22	1429.0
23	1104.0
24	980.0
25	935.0
26	985.0
27	1136.0
28	1421.0
29	1754.0
30	2200.0
31	2942.0
32	3789.0
33	5182.0
34	7673.0
35	11998.0
36	22755.0
37	51032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.75492527173913	21.056244338768117	10.962267889492754	32.2265625
2	18.348052536231883	21.163100090579707	35.799507472826086	24.68933990036232
3	19.209267436594203	23.077304121376812	31.76446444746377	25.948963994565215
4	12.590579710144928	21.983978713768117	36.761916893115945	28.663524682971016
5	11.86028079710145	34.659476902173914	38.7893455615942	14.690896739130435
6	23.745125929700162	43.39426363501263	17.34709041886929	15.51352001641792
7	23.330502476999293	33.05874026893135	21.698513800424628	21.912243453644727
8	20.458566272733705	37.17288231547661	18.19828745311726	24.17026395867242
9	22.977678381859615	18.465229514925902	20.716499879686904	37.84059222352758
10-11	21.573719910691853	27.80264456844422	25.71218291893269	24.911452601931234
12-13	21.50100486865942	24.11437103713768	26.908542798913043	27.476081295289855
14-15	23.706492394461883	26.169136977528368	23.42024534450487	26.704125283504883
16-17	24.831666212587088	26.784090466805605	23.76062811589998	24.62361520470733
18-19	21.22994179360614	26.682235550130034	26.341489305238575	25.746333351025246
20-21	22.573808310688406	26.594698256340582	26.006991621376812	24.824501811594203
22-23	24.83732516214409	25.822396937219366	24.91870681022861	24.421571090407934
24-25	21.122374962404685	29.072059161757164	24.55991366346443	25.245652212373727
26-27	22.335682744565215	29.422129755434785	25.0831493432971	23.1590381567029
28-29	21.598745970581263	28.33420025547665	27.3342321015962	22.73282167234589
30-31	22.362024187784392	27.483352322182984	27.006036331212712	23.148587158819907
32-33	20.30683876811594	29.290859941123188	26.390186254528984	24.012115036231883
34-35	20.622047662013692	29.99805388956708	26.188277338428605	23.191621109990624
36-37	23.322881571869424	26.66968658871493	25.77468078480224	24.232751054613402
38-39	22.876554786885826	25.929333498469543	27.36424919053769	23.829862524106936
40-41	20.998924365942027	27.506156589673914	27.16223675271739	24.332682291666664
42-43	19.368911942764743	27.028231955644088	28.21603337308004	25.386822728511127
44-45	18.78509732596857	25.811462561083058	29.834363621566347	25.569076491382027
46-47	20.162055056259288	26.26565706602505	28.813955134102326	24.75833274361333
48-49	19.893215013586957	26.93755661231884	28.26759227807971	24.901636096014492
50-51	22.274432099639093	26.63364234661383	26.38489845021584	24.707027103531242
52-53	17.895627125481717	27.694164192467348	28.292218569411464	26.117990112639472
54-55	18.079809764434867	26.694550897567915	28.25826176498689	26.96737757301033
56-57	19.23542400951208	27.370235112602092	27.273981910059874	26.12035896782595
58-59	18.992802191191355	27.230648368992092	27.162704451034376	26.613844988782176
60-61	17.923880575885843	26.396183411429945	27.279548124973456	28.400387887710753
62-63	18.924571367907	26.38781251658793	26.777783675708193	27.90983243979688
64-65	19.718289920724803	26.010758776896942	26.185234994337485	28.08571630804077
66-67	18.962179684541695	28.486798101729466	24.642835656626783	27.90818655710206
68-69	19.27437160326087	29.71757529438406	23.901013360507246	27.107039741847828
70-71	20.14655514039855	29.962423573369566	23.497650588768117	26.39337069746377
72-73	20.652270065425157	29.496519976080364	23.34075219469734	26.510457763797135
74-75	21.800456483660362	29.510076257541712	22.762610803446627	25.9268564553513
76	21.54285997353318	29.640008775095712	23.56891634644644	25.24821490492467
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.5
15	8.0
16	11.5
17	10.0
18	13.0
19	11.0
20	21.0
21	36.0
22	41.0
23	57.5
24	135.0
25	201.0
26	278.5
27	390.0
28	516.5
29	609.0
30	742.0
31	1038.0
32	1527.5
33	1854.0
34	2065.0
35	2728.0
36	3710.5
37	4241.0
38	4782.5
39	6070.5
40	7333.0
41	8287.5
42	8726.0
43	9206.5
44	10124.5
45	10584.0
46	10606.0
47	10705.0
48	10386.5
49	9521.0
50	9073.0
51	8497.5
52	7103.5
53	5845.5
54	5406.0
55	4764.5
56	3651.5
57	2786.5
58	2393.0
59	2030.0
60	1429.5
61	1094.0
62	996.0
63	850.5
64	623.0
65	477.0
66	383.5
67	354.0
68	318.0
69	256.5
70	203.0
71	175.0
72	165.5
73	144.0
74	105.5
75	79.0
76	62.0
77	48.0
78	44.0
79	37.0
80	30.0
81	20.5
82	16.5
83	15.0
84	11.5
85	5.5
86	3.5
87	4.0
88	4.5
89	5.5
90	5.5
91	3.5
92	2.0
93	1.5
94	3.0
95	5.0
96	5.0
97	3.0
98	2.5
99	5.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.002122961956521739
7	0.008491847826086956
8	0.0014153079710144928
9	0.00990715579710145
10-11	0.0024767889492753625
12-13	0.0
14-15	3.538269927536232E-4
16-17	3.538269927536232E-4
18-19	0.0031844429347826085
20-21	0.0
22-23	0.001769134963768116
24-25	0.0031844429347826085
26-27	0.0
28-29	0.005307404891304348
30-31	7.076539855072464E-4
32-33	0.0
34-35	0.0031844429347826085
36-37	0.0198143115942029
38-39	0.010260982789855072
40-41	0.0
42-43	3.538269927536232E-4
44-45	0.006015058876811595
46-47	0.0014153079710144928
48-49	0.0
50-51	0.0014153079710144928
52-53	0.01450690670289855
54-55	0.009553328804347826
56-57	0.012737771739130434
58-59	0.013445425724637682
60-61	0.02406023550724638
62-63	0.013799252717391304
64-65	0.022644927536231884
66-67	0.01804517663043478
68-69	0.0
70-71	0.0
72-73	0.0038920969202898546
74-75	0.010260982789855072
76	0.002122961956521739
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	141312.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.00498188405797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2886492289674	46.57141644021739
2	7.133271520593403	8.275305706521738
3	4.043041186804606	7.035495923913043
4	2.399716962717158	5.567821557971015
5	1.539625219597892	4.465296648550725
6	1.0028303728284207	3.4901494565217392
7	0.7368729260199102	2.9919610507246377
8	0.5624146008198322	2.6098278985507246
9	0.41845598282256485	2.1845278532608696
>10	1.8726820222525864	16.728940217391305
>50	0.0024399765762248686	0.0792572463768116
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0014153079710144928	0.0
2	0.0	0.0	0.0	0.0014153079710144928	0.0
3	0.0	0.0	0.0	0.0014153079710144928	0.0
4	0.0	0.0	0.0	0.003538269927536232	0.0
5	0.0	0.0	0.0	0.007076539855072464	0.0
6	0.0	0.0	0.0	0.007076539855072464	0.0
7	0.0	0.0	0.0	0.007076539855072464	0.0
8	0.0	0.0	0.0	0.007076539855072464	0.0
9	0.0	0.0	0.0	0.009199501811594202	0.0
10	0.0	0.0	0.0	0.00990715579710145	0.0
11	0.0	0.0	0.0	0.00990715579710145	0.0
12	0.0	0.0	0.0	0.011322463768115942	0.0
13	0.0	0.0	0.0	0.012737771739130434	0.0
14	0.0	0.0	0.0	0.01556838768115942	0.0
15	0.0	0.0	0.0	0.01556838768115942	0.0
16	0.0	0.0	0.0	0.01556838768115942	0.0
17	0.0	0.0	0.0	0.01556838768115942	0.0
18	0.0	0.0	0.0	0.01556838768115942	0.0
19	0.0	0.0	0.0	0.0198143115942029	0.0
20	0.0	0.0	0.0	0.0198143115942029	0.0
21	0.0	0.0	0.0	0.026183197463768116	0.0
22	0.0	0.0	0.0	0.04104393115942029	0.0
23	0.0	0.0	0.0	0.04458220108695652	0.0
24	0.0	0.0	0.0	0.055904664855072464	0.0
25	0.0	0.0	0.0	0.05661231884057971	0.0
26	0.0	0.0	0.0	0.059442934782608696	0.0
27	0.0	0.0	0.0	0.07430366847826086	0.0
28	0.0	0.0	0.0	0.09270267210144928	0.0
29	0.0	0.0	0.0	0.12030117753623189	0.0
30	0.0	0.0	0.0	0.1450690670289855	0.0
31	0.0	0.0	0.0	0.18045176630434784	0.0
32	0.0	0.0	0.0	0.21088088768115942	0.0
33	0.0	0.0	0.0	0.244140625	0.0
34	0.0	0.0	0.0	0.3141983695652174	0.0
35	0.0	0.0	0.0	0.4104393115942029	0.0
36	0.0	0.0	0.0	0.5632925724637681	0.0
37	0.0	0.0	0.0	0.9418874547101449	0.0
38	0.0	0.0	0.0	1.4450294384057971	0.0
39	0.0	0.0	0.0	2.0585654438405796	0.0
40	0.0	0.0	0.0	2.718806612318841	0.0
41	0.0	0.0	0.0	3.4052309782608696	0.0
42	0.0	0.0	0.0	4.1482676630434785	0.0
43	0.0	0.0	0.0	4.9337635869565215	0.0
44	0.0	0.0	0.0	5.762426403985507	0.0
45	0.0	0.0	0.0	6.685914855072464	0.0
46	0.0	0.0	0.0	7.697860054347826	0.0
47	0.0	0.0	0.0	8.672299592391305	0.0
48	0.0	0.0	0.0	9.780485733695652	0.0
49	0.0	0.0	0.0	10.696897644927537	0.0
50	0.0	0.0	0.0	11.658599411231885	0.0
51	0.0	0.0	0.0	12.621008831521738	0.0
52	0.0	0.0	0.0	13.439056838768115	0.0
53	0.0	0.0	0.0	14.208984375	0.0
54	0.0	0.0	0.0	14.959097599637682	0.0
55	0.0	0.0	0.0	15.755208333333334	0.0
56	0.0	0.0	0.0	16.451539855072465	0.0
57	0.0	0.0	0.0	17.217221467391305	0.0
58	0.0	0.0	0.0	17.929121376811594	0.0
59	0.0	0.0	0.0	18.663666213768117	0.0
60	0.0	0.0	0.0	19.4435009057971	0.0
61	0.0	0.0	0.0	20.19219882246377	0.0
62	0.0	0.0	0.0	20.82625679347826	0.0
63	0.0	0.0	0.0	21.52754189311594	0.0
64	0.0	0.0	0.0	22.163722826086957	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAGATC	30	8.342275E-6	58.333332	38
CTTACGC	30	8.342275E-6	58.333332	12
CTCGGTG	75	0.0	56.0	44
GTGTACA	25	2.3838425E-4	55.999996	36
GCGGAGA	25	2.3838425E-4	55.999996	36
GAGATCT	25	2.3838425E-4	55.999996	39
TCCTCAG	20	0.006939489	52.5	16
GCGTTGA	20	0.006939489	52.5	36
ACGATAT	20	0.006939489	52.5	25
TCTAAGA	20	0.006939489	52.5	17
TCTCGGT	80	0.0	52.5	43
TGTTCGA	20	0.006939489	52.5	37
AACGACT	20	0.006939489	52.5	6
CAATGTA	20	0.006939489	52.5	45
GACGACT	20	0.006939489	52.5	23
ACGACTC	20	0.006939489	52.5	24
CTGTGTA	20	0.006939489	52.5	34
GACTATC	20	0.006939489	52.5	38
TCGGTGG	75	0.0	51.333332	45
TCGCCGT	55	2.2628228E-9	50.90909	52
>>END_MODULE