FastQCFastQC Report
Wed 25 May 2016
SRR1779811_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779811_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141312
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT1793912.694604846014492No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG23241.6445878623188404No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC13790.9758548460144928No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT4650.32905910326086957Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC4540.32127490942028986No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT2980.2108808876811594No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT2890.2045120018115942No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1930.13657721920289856No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT1420.10048686594202898No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT1420.10048686594202898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT353.6816346E-969.9996539
AAACTGG150.002220901769.9996416
CGAGTTC150.002220901769.9996445
AGATATC301.0178337E-769.9996435
TAAACAG150.002220901769.9996422
ATATCGT301.0178337E-769.9996437
CAAGGCT150.002220901769.9996416
CCGAGTT150.002220901769.9996444
AAACAGG150.002220901769.9996423
AACTGGG150.002220901769.9996417
ACAGATA301.0178337E-769.9996433
GCTAAAC150.002220901769.9996456
GCTCGGA352.9572948E-759.999711
GCATGTA352.9572948E-759.99972
GGCTTAT252.379734E-456.019541
CTTTATA850.053.529142
CTTAACT200.00693962752.49973337
GATACCC200.00693962752.49973328
GTTCTGC407.4375566E-752.49973324
ATTAGAT200.00693962752.49973324
Produced by FastQC (version 0.11.5)