FastQCFastQC Report
Wed 25 May 2016
SRR1779810_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779810_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192156
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGT150.002218470770.02865633
GGTCGTC150.002223059369.99219550
CGATACT252.3873242E-455.99375525
ACGCTTC452.7011993E-854.41003415
CGGTGGT653.6379788E-1253.8401546
TGGACTA200.00693203552.5214935
GTCGTCG200.00693203552.5214951
GTACTCG200.00693918152.5078123
CACGAAC200.006946332352.4941423
TTACGCT200.00696065252.4668213
TGGTCGC651.8371793E-1048.45613549
TTACGTG451.6736831E-646.6857732
CGACCCG305.870339E-446.6614624
GACGATC305.870339E-446.6614623
GACGAAT305.870339E-446.6614623
CGACGAT900.046.64931522
GGACGAT305.8778794E-446.64931522
TCGGTGG751.4551915E-1146.64931545
ACGATAC551.3061253E-744.54048524
GTAGATC1450.043.43211738
Produced by FastQC (version 0.11.5)