##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779810_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	192156
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.429177335081913	34.0	27.0	34.0	12.0	34.0
2	30.09379358437936	34.0	31.0	34.0	12.0	34.0
3	29.81021149482712	34.0	31.0	34.0	11.0	34.0
4	29.823669310352006	34.0	31.0	34.0	12.0	34.0
5	30.555996169778723	34.0	32.0	34.0	21.0	34.0
6	33.145912695934555	38.0	34.0	38.0	12.0	38.0
7	33.44984804013406	38.0	34.0	38.0	12.0	38.0
8	32.704141426757424	38.0	33.0	38.0	11.0	38.0
9	33.58119444617915	38.0	34.0	38.0	21.0	38.0
10-11	32.209743645787796	37.0	30.5	38.0	11.5	38.0
12-13	33.09983815233456	38.0	34.0	38.0	11.0	38.0
14-15	31.870774266741606	37.0	29.0	38.0	11.0	38.0
16-17	32.48988582193634	37.5	30.5	38.0	11.5	38.0
18-19	33.329276733487376	38.0	34.0	38.0	16.5	38.0
20-21	32.066695809654654	37.0	30.0	38.0	16.0	38.0
22-23	32.93959855534045	38.0	32.5	38.0	16.0	38.0
24-25	33.2418529736256	38.0	34.0	38.0	16.0	38.0
26-27	32.20994660588272	37.0	30.5	38.0	11.0	38.0
28-29	33.37323060430067	38.0	34.0	38.0	16.0	38.0
30-31	33.729568683777764	38.0	35.0	38.0	21.0	38.0
32-33	33.84028081350569	38.0	35.5	38.0	21.0	38.0
34-35	33.77852369949416	38.0	34.5	38.0	21.0	38.0
36-37	33.32581340161119	38.0	34.0	38.0	16.0	38.0
38-39	33.25721549157976	38.0	34.0	38.0	16.0	38.0
40-41	33.26646058410874	38.0	34.0	38.0	15.0	38.0
42-43	33.63434657257645	38.0	35.0	38.0	16.0	38.0
44-45	33.711414163492165	38.0	34.0	38.0	21.0	38.0
46-47	33.60891671350361	38.0	34.0	38.0	16.0	38.0
48-49	33.37967588834073	38.0	34.0	38.0	16.0	38.0
50-51	32.754577010345756	37.5	32.5	38.0	11.0	38.0
52-53	33.32933137658985	38.0	34.0	38.0	20.5	38.0
54-55	33.434927871104726	38.0	34.0	38.0	16.0	38.0
56-57	33.496091717146484	38.0	34.0	38.0	16.0	38.0
58-59	33.71361289785383	38.0	35.0	38.0	20.0	38.0
60-61	33.60962707383584	38.0	34.0	38.0	21.0	38.0
62-63	33.6226139178584	38.0	34.0	38.0	20.0	38.0
64-65	33.6434199296405	38.0	34.5	38.0	20.5	38.0
66-67	33.51433470721705	38.0	34.0	38.0	20.5	38.0
68-69	32.8989466891484	38.0	33.0	38.0	11.0	38.0
70-71	32.98877214346677	37.5	33.5	38.0	11.0	38.0
72-73	33.20189585545078	38.0	34.0	38.0	15.0	38.0
74-75	33.23247777847166	38.0	34.0	38.0	14.0	38.0
76	32.345333999458774	37.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	40.0
13	498.0
14	1648.0
15	2951.0
16	3739.0
17	4021.0
18	4077.0
19	3632.0
20	2829.0
21	2108.0
22	1652.0
23	1447.0
24	1413.0
25	1422.0
26	1595.0
27	1865.0
28	2291.0
29	2740.0
30	3505.0
31	4403.0
32	5731.0
33	7967.0
34	11115.0
35	17339.0
36	31523.0
37	70602.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.537958741855576	19.574200129061804	9.999687753700119	31.8881533753825
2	22.469243739461685	20.183080413830428	31.82050001040821	25.527175836299676
3	17.74755927475593	23.974270904889778	30.424238639438787	27.85393118091551
4	15.229292866212868	20.843481338079478	32.417931264181185	31.509294531526464
5	14.230625117092362	33.96719332209246	35.2281479631133	16.574033597701867
6	29.407233931824095	37.797553994275304	17.017434296122822	15.777777777777777
7	27.018080754457735	31.131790692106716	20.104820493603555	21.745308059832
8	23.71103975519253	36.27147399701276	19.37070324900729	20.646782998787412
9	24.121599467052498	17.712697578315698	20.46643315516371	37.69926979946809
10-11	23.345424644416575	26.282468292835247	25.287667383124557	25.084439679623628
12-13	23.13510897395866	23.168935656445804	26.374664335227628	27.32129103436791
14-15	23.95377701913294	25.177265287748725	23.355823798954493	27.513133894163843
16-17	24.934428277024917	26.66062990486896	23.9794750098878	24.42546680821832
18-19	22.01292229707752	26.278633452772205	25.93462868562596	25.77381556452431
20-21	23.264170777909616	26.173265471804157	25.55892087678761	25.003642873498617
22-23	24.91042177876196	25.64292052323569	24.91042177876196	24.536235919240386
24-25	22.05637826993185	28.34656528674437	24.479243918115415	25.117812525208365
26-27	22.66457460012646	28.297281614534135	25.36318431262344	23.674959472715965
28-29	22.27993296763919	27.364087724959145	26.808000166540026	23.547979140861635
30-31	22.480868259655644	27.06884304036595	26.462039660793792	23.988249039184613
32-33	21.436749307854036	28.11465684131643	26.20396969129249	24.244624159537043
34-35	21.370114679316867	28.83038546510569	25.99580003591047	23.803699819666967
36-37	23.537020505786856	26.764489255217537	25.73334409069497	23.96514614830064
38-39	23.334270159989174	26.043520802756355	27.028489939522636	23.59371909773184
40-41	21.832374905675106	26.890010668470765	26.768754390986444	24.508860034867684
42-43	20.416643898529568	26.574188998956565	27.396964421858456	25.61220268065541
44-45	20.201667989435197	25.311804733342875	28.784657619797294	25.701869657424638
46-47	20.96513140775436	25.66952901379131	28.151704397606036	25.2136351808483
48-49	20.711297071129707	26.132933658069486	28.331928224983866	24.82384104581694
50-51	22.406193078324225	26.298204527712727	26.755659640905545	24.539942753057506
52-53	18.97747914205264	27.35917307281935	27.50386451052136	26.159483274606654
54-55	19.20706794701642	26.27449449605746	27.50930335441226	27.009134202513856
56-57	19.50741557126834	26.837887987008763	27.233714760096706	26.420981681626195
58-59	19.361890386717327	26.669182324467812	27.35595690417946	26.612970384635403
60-61	19.35395714910363	25.80163241509984	27.571470214670914	27.27294022112562
62-63	19.663666977218668	26.53736252869136	26.822844829829645	26.97612566426032
64-65	20.314813899075286	26.061156555890136	25.995570326916116	27.628459218118458
66-67	19.65677612111212	27.630719422026395	25.057581349108236	27.65492310775325
68-69	20.14030376597998	28.568417601461327	24.16466957578407	27.126609056774626
70-71	20.56725039681507	28.786916811948686	24.21742863833884	26.4284041528974
72-73	21.6738834011637	28.851238121012145	23.428018277767947	26.04686020005621
74-75	22.170755254618367	29.006638371400093	23.065933522604553	25.756672851376987
76	21.851971356009827	28.94010991298555	23.628169365918648	25.579749365085974
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	1.5
10	1.0
11	0.5
12	0.0
13	2.0
14	5.5
15	8.0
16	16.0
17	23.0
18	27.0
19	51.0
20	89.5
21	108.0
22	108.0
23	164.0
24	296.5
25	373.0
26	470.5
27	677.5
28	940.5
29	1094.0
30	1285.5
31	1650.0
32	2408.0
33	2993.0
34	3289.0
35	4046.5
36	5036.5
37	5565.0
38	6256.0
39	7727.5
40	9138.5
41	10298.5
42	10828.0
43	11198.0
44	12121.5
45	12712.5
46	12750.0
47	12891.5
48	12360.5
49	11415.0
50	11142.0
51	10572.0
52	9135.0
53	7771.5
54	7275.0
55	6733.0
56	5515.5
57	4363.5
58	3887.0
59	3478.0
60	2814.5
61	2281.0
62	2002.0
63	1875.0
64	1637.0
65	1432.5
66	1217.5
67	1096.0
68	1049.0
69	896.0
70	720.5
71	651.0
72	634.0
73	611.5
74	544.0
75	482.0
76	430.5
77	354.5
78	284.5
79	239.0
80	234.0
81	211.0
82	172.5
83	152.0
84	137.5
85	97.0
86	66.0
87	61.0
88	48.0
89	32.5
90	29.5
91	22.5
92	16.0
93	13.5
94	9.0
95	7.5
96	8.0
97	10.0
98	8.5
99	7.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.003122462998813464
7	0.009367388996440393
8	0.00260205249901122
9	0.00884697849663815
10-11	0.0036428734986157078
12-13	0.0
14-15	2.60205249901122E-4
16-17	0.0
18-19	0.00390307874851683
20-21	0.0
22-23	0.002862257748912342
24-25	0.00390307874851683
26-27	7.80615749703366E-4
28-29	0.00520410499802244
30-31	7.80615749703366E-4
32-33	0.0
34-35	0.005984720747725806
36-37	0.02055621474218864
38-39	0.009367388996440393
40-41	5.20410499802244E-4
42-43	7.80615749703366E-4
44-45	0.004423489248319075
46-47	0.003122462998813464
48-49	0.0
50-51	0.003122462998813464
52-53	0.011969441495451613
54-55	0.010928620495847124
56-57	0.013790878244759466
58-59	0.013530672994858346
60-61	0.024979703990507713
62-63	0.0130102624950561
64-65	0.02211744624159537
66-67	0.018994983242781906
68-69	7.80615749703366E-4
70-71	5.20410499802244E-4
72-73	0.00520410499802244
74-75	0.008066362746934782
76	0.002081641999208976
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	192156.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.64342924188694
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.0051860720937	42.485509500492036
2	10.194201595678793	11.548690735247254
3	5.7091780486136	9.70162268477936
4	3.168908376683749	7.17991349634835
5	1.894657426401928	5.365994693500661
6	1.0563161925565687	3.590002290608246
7	0.6806096860606515	2.698644661560358
8	0.45240917080039106	2.050080548369016
9	0.3218679540506055	1.640853421244675
>10	1.5086935166436637	13.478476992817468
>50	0.00797196041633009	0.26021097503257423
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002081641999208976	0.0
2	0.0	0.0	0.0	0.002081641999208976	0.0
3	0.0	0.0	0.0	0.002081641999208976	0.0
4	0.0	0.0	0.0	0.00260205249901122	0.0
5	0.0	0.0	0.0	0.004683694498220196	0.0
6	0.0	0.0	0.0	0.004683694498220196	0.0
7	0.0	0.0	0.0	0.004683694498220196	0.0
8	0.0	0.0	0.0	0.004683694498220196	0.0
9	0.0	0.0	0.0	0.006244925997626928	0.0
10	0.0	0.0	0.0	0.006244925997626928	0.0
11	0.0	0.0	0.0	0.006765336497429172	0.0
12	0.0	0.0	0.0	0.006765336497429172	0.0
13	0.0	0.0	0.0	0.00780615749703366	0.0
14	0.0	0.0	0.0	0.00780615749703366	0.0
15	0.0	0.0	0.0	0.008326567996835904	0.0
16	0.0	0.0	0.0	0.008326567996835904	0.0
17	0.0	0.0	0.0	0.008326567996835904	0.0
18	0.0	0.0	0.0	0.009887799496242636	0.0
19	0.0	0.0	0.0	0.009887799496242636	0.0
20	0.0	0.0	0.0	0.009887799496242636	0.0
21	0.0	0.0	0.0	0.009887799496242636	0.0
22	0.0	0.0	0.0	0.016653135993671807	0.0
23	0.0	0.0	0.0	0.018734777992880786	0.0
24	0.0	0.0	0.0	0.019775598992485273	0.0
25	0.0	0.0	0.0	0.023418472491100982	0.0
26	0.0	0.0	0.0	0.028622577489123422	0.0
27	0.0	0.0	0.0	0.06557172297508275	0.0
28	0.0	0.0	0.0	0.09887799496242636	0.0
29	0.0	0.0	0.0	0.1311434459501655	0.0
30	0.0	0.0	0.0	0.1644497179375091	0.0
31	0.0	0.0	0.0	0.2055621474218864	0.0
32	0.0	0.0	0.0	0.24875621890547264	0.0
33	0.0	0.0	0.0	0.2909094693894544	0.0
34	0.0	0.0	0.0	0.3601240658631529	0.0
35	0.0	0.0	0.0	0.47253273382043753	0.0
36	0.0	0.0	0.0	0.6302171152605175	0.0
37	0.0	0.0	0.0	0.9268511001477966	0.0
38	0.0	0.0	0.0	1.341618268490185	0.0
39	0.0	0.0	0.0	1.8401715273007349	0.0
40	0.0	0.0	0.0	2.4266741605778637	0.0
41	0.0	0.0	0.0	3.2785861487541372	0.0
42	0.0	0.0	0.0	4.227294489893628	0.0
43	0.0	0.0	0.0	5.193176377526593	0.0
44	0.0	0.0	0.0	6.007098399217303	0.0
45	0.0	0.0	0.0	6.784071275422053	0.0
46	0.0	0.0	0.0	7.515768438144008	0.0
47	0.0	0.0	0.0	8.290139261849747	0.0
48	0.0	0.0	0.0	9.020275193072296	0.0
49	0.0	0.0	0.0	9.732196756801764	0.0
50	0.0	0.0	0.0	10.494598139012053	0.0
51	0.0	0.0	0.0	11.251795416224319	0.0
52	0.0	0.0	0.0	12.230167155852536	0.0
53	0.0	0.0	0.0	13.288682112450301	0.0
54	0.0	0.0	0.0	14.308686692062698	0.0
55	0.0	0.0	0.0	15.127292408251629	0.0
56	0.0	0.0	0.0	15.78821374300048	0.0
57	0.0	0.0	0.0	16.46318616124399	0.0
58	0.0	0.0	0.0	17.143883094985323	0.0
59	0.0	0.0	0.0	17.75016132725494	0.0
60	0.0	0.0	0.0	18.402756094006953	0.0
61	0.0	0.0	0.0	18.983013801286454	0.0
62	0.0	0.0	0.0	19.6892108495181	0.0
63	0.0	0.0	0.0	20.33399945877308	0.0
64	0.0	0.0	0.0	20.991277920023315	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGT	15	0.0022184707	70.028656	33
GGTCGTC	15	0.0022230593	69.992195	50
CGATACT	25	2.3873242E-4	55.993755	25
ACGCTTC	45	2.7011993E-8	54.410034	15
CGGTGGT	65	3.6379788E-12	53.84015	46
TGGACTA	20	0.006932035	52.52149	35
GTCGTCG	20	0.006932035	52.52149	51
GTACTCG	20	0.006939181	52.507812	3
CACGAAC	20	0.0069463323	52.49414	23
TTACGCT	20	0.006960652	52.46682	13
TGGTCGC	65	1.8371793E-10	48.456135	49
TTACGTG	45	1.6736831E-6	46.68577	32
CGACCCG	30	5.870339E-4	46.66146	24
GACGATC	30	5.870339E-4	46.66146	23
GACGAAT	30	5.870339E-4	46.66146	23
CGACGAT	90	0.0	46.649315	22
GGACGAT	30	5.8778794E-4	46.649315	22
TCGGTGG	75	1.4551915E-11	46.649315	45
ACGATAC	55	1.3061253E-7	44.540485	24
GTAGATC	145	0.0	43.432117	38
>>END_MODULE