FastQCFastQC Report
Wed 25 May 2016
SRR1779810_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779810_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192156
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT183869.568267449364058No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG23441.21984221153646No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC12520.6515539457524094No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT4930.2565623764025063RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC4390.22846020941318512No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT3390.17641915943296071No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT2590.1347863194487812No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC2300.11969441495451613RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT2090.108765794458669No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCA150.002227228469.95914511
ATCGTAT308.382625E-658.29928639
TCCGTAT308.382625E-658.29928613
CGTCTTC27350.054.05653448
CCGTCTT27250.053.9977847
TATGCCG27300.053.9428643
TCGTATG27500.053.93214440
TCTCGTA25950.053.9184238
GCCGTCT27300.053.89888446
CGTATGC27500.053.80494341
CTCGTAT27250.053.7851139
ATCTCGT25950.053.78362337
GATCTCG25950.053.64882736
TTCTGCT27800.053.5735552
TCTTCTG27750.053.54379750
CTTCTGC27900.053.3815351
ATGCCGT27550.053.3263944
TGCCGTC27650.053.1888945
CTGCTTG27900.053.13032554
GTCTTCT27900.053.13032549