FastQCFastQC Report
Wed 25 May 2016
SRR1779809_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779809_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219476
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGAAA200.00694269752.50359338
ACGATAA200.006955228352.47966424
CCACGTT200.006955228352.47966413
CGATAAG200.006955228352.47966425
GTAGATT503.4663426E-642.00287238
GACGATA503.477233E-641.9837323
GTGGTCG1100.041.36646748
TCGGTGG1200.040.80821245
CCGTATC953.6379788E-1240.5568355
CGCCGTA1050.040.03011753
GCGTAGA350.00125172240.0118636
GTCTAAA350.00125453339.9936181
GGTCAAC350.00125453339.9936181
TATGCCT350.00125453339.9936186
TACCACG350.001255940339.98458
ACGAATA801.4188117E-939.3597524
CGTATCC459.14924E-538.9092956
AGGTGTA1007.2759576E-1238.51141434
TCGCCGT1100.038.2018552
TCTCGGT1100.038.1670343