##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779809_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219476
Sequences flagged as poor quality	0
Sequence length	76
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.99486959849824	34.0	23.0	34.0	12.0	34.0
2	29.77637646029634	34.0	31.0	34.0	12.0	34.0
3	29.288090725181796	34.0	27.0	34.0	11.0	34.0
4	29.5995644170661	34.0	27.0	34.0	12.0	34.0
5	30.088752300934953	34.0	32.0	34.0	11.0	34.0
6	32.475760447611584	37.0	31.0	38.0	12.0	38.0
7	32.85614372414296	37.0	33.0	38.0	12.0	38.0
8	32.086984453881065	37.0	31.0	38.0	11.0	38.0
9	32.99438207366637	38.0	34.0	38.0	12.0	38.0
10-11	31.61052461316955	37.0	28.5	38.0	11.5	38.0
12-13	32.444116896608286	37.0	31.0	38.0	11.0	38.0
14-15	31.214898212105197	37.0	27.0	38.0	11.0	38.0
16-17	31.855870345732562	37.0	28.5	38.0	11.0	38.0
18-19	32.67613087535767	37.5	32.0	38.0	11.0	38.0
20-21	31.421392316244145	37.0	27.5	38.0	11.0	38.0
22-23	32.299884725436954	37.0	31.0	38.0	11.0	38.0
24-25	32.603751663051995	37.5	32.0	38.0	11.0	38.0
26-27	31.512213180484427	37.0	26.5	38.0	11.0	38.0
28-29	32.67544742933168	37.5	32.5	38.0	11.0	38.0
30-31	33.04503909311269	38.0	34.0	38.0	11.0	38.0
32-33	33.15825420547121	38.0	34.0	38.0	11.0	38.0
34-35	33.09262971805573	38.0	34.0	38.0	11.0	38.0
36-37	32.62434161366163	38.0	32.5	38.0	11.0	38.0
38-39	32.55457088702181	38.0	32.5	38.0	11.0	38.0
40-41	32.553021742696245	37.5	32.0	38.0	11.0	38.0
42-43	32.90716524813647	38.0	33.0	38.0	11.0	38.0
44-45	32.97517496218265	38.0	34.0	38.0	11.0	38.0
46-47	32.90753658714392	38.0	34.0	38.0	11.0	38.0
48-49	32.62120231824892	38.0	32.5	38.0	11.0	38.0
50-51	32.010279028230876	37.5	29.5	38.0	11.0	38.0
52-53	32.55337030016949	37.0	32.0	38.0	11.0	38.0
54-55	32.676875831526	37.5	32.5	38.0	11.0	38.0
56-57	32.72755791065994	38.0	33.0	38.0	11.0	38.0
58-59	32.93199712041408	38.0	34.0	38.0	11.0	38.0
60-61	32.8370687455576	38.0	34.0	38.0	11.0	38.0
62-63	32.8358476553245	38.0	34.0	38.0	11.0	38.0
64-65	32.82272776977893	38.0	33.5	38.0	11.0	38.0
66-67	32.7180830705863	38.0	32.5	38.0	11.0	38.0
68-69	32.09913840237657	37.0	30.5	38.0	11.0	38.0
70-71	32.15342679837431	37.0	31.0	38.0	11.0	38.0
72-73	32.32215595327052	37.0	31.0	38.0	11.0	38.0
74-75	32.33829894840438	37.5	31.5	38.0	11.0	38.0
76	31.33283821465673	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	49.0
13	557.0
14	2062.0
15	3709.0
16	4795.0
17	5463.0
18	5629.0
19	4968.0
20	3965.0
21	2953.0
22	2432.0
23	2153.0
24	2065.0
25	2216.0
26	2402.0
27	2697.0
28	3167.0
29	3826.0
30	4526.0
31	5718.0
32	7172.0
33	9341.0
34	13099.0
35	19082.0
36	33706.0
37	71724.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.10570680031894	17.35505182822645	11.725253445722746	30.813987925731862
2	20.42865734750041	24.42453844611712	28.513368204268346	26.633436002114124
3	19.3688603765332	22.664437113852994	29.339882265031257	28.62682024458255
4	14.44075889846726	20.840547485829887	27.3487761759828	37.36991743972006
5	15.648707142043513	31.36894862740631	32.56726278619433	20.41508144435585
6	29.59155776696375	34.537609127526565	17.83540197204148	18.035431133468204
7	27.42056747606658	30.229697033212886	17.353266837690178	24.99646865303035
8	24.542882267978293	34.77876549735047	19.038788370323456	21.639563864347778
9	24.265111298443873	17.798181859606753	18.17593584106081	39.76077100088857
10-11	24.529436690952338	25.42158189464667	22.779547183910402	27.269434230490592
12-13	24.753731615301902	21.670934407406733	24.40859137217737	29.166742605114
14-15	25.449082015988118	23.863255807595838	21.265243728798886	29.42241844761716
16-17	26.381304519183235	24.64762581700463	21.2645602812159	27.706509382596234
18-19	23.55396283327372	24.379586322535374	23.69384495866187	28.372605885529033
20-21	25.75065155187811	23.809664838068855	23.23602580692194	27.203657803131094
22-23	26.498991901035435	23.930560776407066	21.845333697843692	27.725113624713803
24-25	23.627213556202058	26.63557682888211	21.656342623074064	28.080866991841763
26-27	25.015776248373967	26.199507462108528	22.492943403705652	26.291772885811852
28-29	24.099333599134248	25.49661103833229	23.453437375405823	26.95061798712764
30-31	24.41024168984693	25.344970167513004	23.303967868850965	26.9408202737891
32-33	23.112094260875903	26.208332573948862	23.6490550219614	27.03051814321384
34-35	22.769606502998162	26.6997922248346	23.564027557548254	26.966573714618985
36-37	24.899569109896344	24.50217495824399	23.52053848730458	27.077717444555084
38-39	24.99447488636493	23.568425969173283	24.93045192011939	26.5066472243424
40-41	23.392467006417604	25.149846565318523	24.30419023622334	27.15349619204053
42-43	21.666476819683336	24.386376580476135	25.381706344686183	28.565440255154346
44-45	21.84407956639912	22.98686120078646	27.055628586855736	28.113430645958687
46-47	21.664137858760384	24.05420306099667	26.38116197584191	27.90049710440104
48-49	21.77846324882903	24.462811423572507	26.379877526472146	27.378847801126323
50-51	23.65090683756205	24.46308518418109	24.92578763074021	26.96022034751665
52-53	19.34226325418832	26.029678809931212	26.08299403735256	28.545063898527907
54-55	20.56785094254508	24.877539768248283	25.562861400079285	28.991747889127357
56-57	20.764295218825758	25.53248209110297	24.922075791545907	28.78114689852536
58-59	20.444256090920067	25.077752790556197	25.579699743216427	28.898291375307313
60-61	19.779100450731697	24.541634574630507	25.779209829505835	29.900055145131958
62-63	20.905122327991215	25.028138399916156	24.835612505752994	29.23112676633964
64-65	21.703345531771387	24.27162564315239	24.15085654910289	29.874172275973333
66-67	20.93075718872409	26.842156833702862	22.506477200482173	29.720608777090877
68-69	21.469317829739925	27.444914250305274	21.97210628952596	29.11366163042884
70-71	22.041030197405142	28.027201585620066	21.645764275706526	28.286003941268266
72-73	22.88127291077641	28.271413958002512	21.193763940118153	27.65354919110293
74-75	23.74847347028052	28.62106519876784	20.8464721214662	26.783989209485448
76	22.710061921620625	29.043017072870676	21.310788213476954	26.936132792031746
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	1.0
4	0.0
5	0.5
6	0.5
7	0.5
8	1.0
9	1.5
10	2.5
11	4.0
12	5.0
13	6.5
14	11.5
15	20.5
16	41.5
17	57.0
18	62.0
19	107.5
20	177.0
21	206.0
22	256.5
23	375.0
24	540.0
25	637.0
26	741.0
27	992.5
28	1288.5
29	1437.0
30	1726.0
31	2315.5
32	2818.0
33	3020.0
34	3332.5
35	4103.5
36	4702.5
37	4843.0
38	5276.5
39	6166.0
40	7006.5
41	7776.5
42	8162.0
43	8466.5
44	9097.0
45	9493.5
46	9564.0
47	9724.5
48	9836.5
49	9868.0
50	9948.0
51	9955.5
52	9816.5
53	9541.5
54	9413.0
55	9187.0
56	8817.0
57	8199.5
58	7726.0
59	7405.0
60	6752.0
61	5948.0
62	5476.0
63	5145.5
64	4490.5
65	3863.5
66	3376.5
67	3192.0
68	2997.0
69	2596.5
70	2265.0
71	2139.0
72	2012.5
73	1777.5
74	1581.5
75	1494.0
76	1441.5
77	1275.0
78	1019.5
79	878.0
80	818.0
81	701.5
82	587.5
83	530.0
84	465.0
85	337.0
86	236.5
87	199.0
88	179.0
89	126.5
90	73.0
91	47.0
92	42.0
93	33.5
94	19.0
95	13.5
96	14.0
97	14.5
98	10.0
99	6.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.5563068399278277E-4
2	0.0
3	0.0
4	0.0
5	4.5563068399278277E-4
6	0.003645045471942262
7	0.005923198891906177
8	0.0013668920519783485
9	0.009568244363848438
10-11	0.002278153419963914
12-13	0.0
14-15	2.2781534199639138E-4
16-17	2.2781534199639138E-4
18-19	0.0025059687619603055
20-21	0.0
22-23	0.0015947073939747399
24-25	0.0020503380779675227
26-27	2.2781534199639138E-4
28-29	0.006151014233902568
30-31	2.2781534199639138E-4
32-33	0.0
34-35	0.003645045471942262
36-37	0.021186826805664402
38-39	0.010707321073830395
40-41	6.834460259891742E-4
42-43	4.5563068399278277E-4
44-45	0.0052397528659170025
46-47	0.002278153419963914
48-49	0.0
50-51	0.0020503380779675227
52-53	0.012074213125808746
54-55	0.007745721627877308
56-57	0.014580181887769049
58-59	0.013896735861779875
60-61	0.025059687619603057
62-63	0.01139076709981957
64-65	0.022325903515646357
66-67	0.0239206109096211
68-69	0.0
70-71	0.0015947073939747399
72-73	0.004784122181924219
74-75	0.010935136415826788
76	0.002278153419963914
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	219476.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.70917450249314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.3461646310001	53.14391122729949
2	10.481135368739396	14.403003180699361
3	6.084380128912989	12.541582080509528
4	3.3373381188918776	9.172229887390571
5	1.6067708297938923	5.519994866491213
6	0.6956366447075858	2.86779717669254
7	0.2819860707712431	1.3562521100725606
8	0.11107286025123685	0.6105379629994613
9	0.03791468465801916	0.2344578015937354
>10	0.016923759121050597	0.1260487886970495
>50	6.769031526325287E-4	0.02418491755446841
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.556306839927828E-4	0.0
2	0.0	0.0	0.0	0.0013668920519783485	0.0
3	0.0	0.0	0.0	0.0018225227359711313	0.0
4	0.0	0.0	0.0	0.0063788295758989595	0.0
5	0.0	0.0	0.0	0.00820135231187009	0.0
6	0.0	0.0	0.0	0.00820135231187009	0.0
7	0.0	0.0	0.0	0.00820135231187009	0.0
8	0.0	0.0	0.0	0.008656982995862874	0.0
9	0.0	0.0	0.0	0.015947073939747397	0.0
10	0.0	0.0	0.0	0.018680858043704094	0.0
11	0.0	0.0	0.0	0.02095901146366801	0.0
12	0.0	0.0	0.0	0.023237164883631922	0.0
13	0.0	0.0	0.0	0.024604056935610272	0.0
14	0.0	0.0	0.0	0.025515318303595838	0.0
15	0.0	0.0	0.0	0.033716670615465925	0.0
16	0.0	0.0	0.0	0.034627931983451495	0.0
17	0.0	0.0	0.0	0.03508356266744428	0.0
18	0.0	0.0	0.0	0.036450454719422626	0.0
19	0.0	0.0	0.0	0.04282928429532158	0.0
20	0.0	0.0	0.0	0.046474329767263844	0.0
21	0.0	0.0	0.0	0.04966374455521333	0.0
22	0.0	0.0	0.0	0.0583207275510762	0.0
23	0.0	0.0	0.0	0.061054511655032895	0.0
24	0.0	0.0	0.0	0.06743334123093185	0.0
25	0.0	0.0	0.0	0.0715340173868669	0.0
26	0.0	0.0	0.0	0.08292478448668647	0.0
27	0.0	0.0	0.0	0.15263627913758224	0.0
28	0.0	0.0	0.0	0.19728808616887494	0.0
29	0.0	0.0	0.0	0.25515318303595835	0.0
30	0.0	0.0	0.0	0.30891760374710675	0.0
31	0.0	0.0	0.0	0.3790847290819953	0.0
32	0.0	0.0	0.0	0.4483405930488983	0.0
33	0.0	0.0	0.0	0.5171408263318085	0.0
34	0.0	0.0	0.0	0.64836246332173	0.0
35	0.0	0.0	0.0	0.8210464925549946	0.0
36	0.0	0.0	0.0	1.1208514826222458	0.0
37	0.0	0.0	0.0	1.6015418542346316	0.0
38	0.0	0.0	0.0	2.1574112887058265	0.0
39	0.0	0.0	0.0	2.76704514388817	0.0
40	0.0	0.0	0.0	3.494687346224644	0.0
41	0.0	0.0	0.0	4.434197816617762	0.0
42	0.0	0.0	0.0	5.555960560607994	0.0
43	0.0	0.0	0.0	6.6471960487707085	0.0
44	0.0	0.0	0.0	7.665986258178571	0.0
45	0.0	0.0	0.0	8.579070148900108	0.0
46	0.0	0.0	0.0	9.562321164956533	0.0
47	0.0	0.0	0.0	10.376077566567643	0.0
48	0.0	0.0	0.0	11.109187337112031	0.0
49	0.0	0.0	0.0	11.753449124277825	0.0
50	0.0	0.0	0.0	12.401811587599555	0.0
51	0.0	0.0	0.0	13.186407625435127	0.0
52	0.0	0.0	0.0	14.113616067360441	0.0
53	0.0	0.0	0.0	15.110080373252657	0.0
54	0.0	0.0	0.0	15.927937451019702	0.0
55	0.0	0.0	0.0	16.69567515354754	0.0
56	0.0	0.0	0.0	17.48300497548707	0.0
57	0.0	0.0	0.0	18.186498751571925	0.0
58	0.0	0.0	0.0	18.776996118026574	0.0
59	0.0	0.0	0.0	19.273633563578706	0.0
60	0.0	0.0	0.0	19.753868304507098	0.0
61	0.0	0.0	0.0	20.262807778527037	0.0
62	0.0	0.0	0.0	20.78040423554284	0.0
63	0.0	0.0	0.0	21.35495452805774	0.0
64	0.0	0.0	0.0	21.94135121835645	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGAAA	20	0.006942697	52.503593	38
ACGATAA	20	0.0069552283	52.479664	24
CCACGTT	20	0.0069552283	52.479664	13
CGATAAG	20	0.0069552283	52.479664	25
GTAGATT	50	3.4663426E-6	42.002872	38
GACGATA	50	3.477233E-6	41.98373	23
GTGGTCG	110	0.0	41.366467	48
TCGGTGG	120	0.0	40.808212	45
CCGTATC	95	3.6379788E-12	40.55683	55
CGCCGTA	105	0.0	40.030117	53
GCGTAGA	35	0.001251722	40.01186	36
GTCTAAA	35	0.001254533	39.993618	1
GGTCAAC	35	0.001254533	39.993618	1
TATGCCT	35	0.001254533	39.993618	6
TACCACG	35	0.0012559403	39.9845	8
ACGAATA	80	1.4188117E-9	39.35975	24
CGTATCC	45	9.14924E-5	38.90929	56
AGGTGTA	100	7.2759576E-12	38.511414	34
TCGCCGT	110	0.0	38.20185	52
TCTCGGT	110	0.0	38.16703	43
>>END_MODULE