FastQCFastQC Report
Wed 25 May 2016
SRR1779809_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779809_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219476
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2478611.293262133445115No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG34401.5673695529351728No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC17280.7873298219395287No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT6720.30618381964315RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC5910.26927773423973467No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT2790.1271209608339864No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT2610.1189196085221163No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2600.11846397783812353No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGG200.00696024652.47009720
ATGTGCG200.00696024652.47009728
TCGGAAA200.00696024652.47009729
GTGCGTT200.00696024652.4700976
TATGCCG35150.050.3553743
CGTATGC35400.050.29619241
GCCGTCT35050.050.27982346
CTCGTAT35150.050.2558539
TCGTATG35400.050.1973840
ATGCCGT35150.050.15633444
GCTTGAA35000.050.15144756
GTATGCC35300.050.1413942
TCTCGTA34200.050.11763438
CCGTCTT35200.050.06556747
CGTCTTC35250.049.8951648
TGCTTGA35150.049.83775355
TGCCGTC35350.049.83038345
ATCTCGT34450.049.75393737
CTGCTTG35300.049.7252354
TAATCTC34200.049.4016735