##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779809_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	219476
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.594538810621664	34.0	34.0	34.0	34.0	34.0
2	33.67862545335253	34.0	34.0	34.0	34.0	34.0
3	33.77274508374492	34.0	34.0	34.0	34.0	34.0
4	33.586642730868064	34.0	34.0	34.0	34.0	34.0
5	33.61637718930544	34.0	34.0	34.0	34.0	34.0
6	37.296565455904066	38.0	38.0	38.0	37.0	38.0
7	37.363187774517485	38.0	38.0	38.0	37.0	38.0
8	37.261577575680256	38.0	38.0	38.0	36.0	38.0
9	37.17978275528987	38.0	38.0	38.0	36.0	38.0
10-11	37.12761076381928	38.0	38.0	38.0	35.5	38.0
12-13	37.29720789516849	38.0	38.0	38.0	36.5	38.0
14-15	37.310844921540394	38.0	38.0	38.0	36.5	38.0
16-17	37.23965490531994	38.0	38.0	38.0	36.0	38.0
18-19	37.105731378373946	38.0	38.0	38.0	36.0	38.0
20-21	37.12183792305309	38.0	38.0	38.0	36.0	38.0
22-23	37.107080045198565	38.0	38.0	38.0	36.0	38.0
24-25	37.287926698135564	38.0	38.0	38.0	36.0	38.0
26-27	37.183268330022415	38.0	38.0	38.0	36.0	38.0
28-29	36.701534564143685	38.0	37.0	38.0	34.0	38.0
30-31	36.94310995279666	38.0	37.0	38.0	35.5	38.0
32-33	36.979510288140844	38.0	37.5	38.0	36.0	38.0
34-35	37.0822527292278	38.0	38.0	38.0	36.0	38.0
36-37	37.03956013413767	38.0	38.0	38.0	36.0	38.0
38-39	36.473844520585395	38.0	37.0	38.0	34.0	38.0
40-41	36.821048770708416	38.0	37.0	38.0	35.0	38.0
42-43	37.15581658131185	38.0	38.0	38.0	36.0	38.0
44-45	36.91921440157466	38.0	38.0	38.0	35.5	38.0
46-47	37.0627517359529	38.0	38.0	38.0	36.0	38.0
48-49	36.71707157046784	38.0	37.0	38.0	34.5	38.0
50-51	36.86985820773114	38.0	37.5	38.0	35.0	38.0
52-53	36.785001549144326	38.0	37.5	38.0	35.0	38.0
54-55	36.7831266288797	38.0	37.0	38.0	35.0	38.0
56-57	36.66612750369061	38.0	37.0	38.0	34.5	38.0
58-59	36.74524549381253	38.0	37.0	38.0	35.0	38.0
60-61	36.4192303486486	38.0	37.0	38.0	34.0	38.0
62-63	36.23608276075744	38.0	37.0	38.0	34.0	38.0
64-65	36.58769295959467	38.0	37.0	38.0	34.0	38.0
66-67	36.34467094352002	38.0	37.0	38.0	33.5	38.0
68-69	36.444057664619365	38.0	37.0	38.0	34.0	38.0
70-71	35.52556999398568	38.0	36.5	38.0	29.0	38.0
72-73	33.91019974849186	38.0	35.5	38.0	21.5	38.0
74-75	33.66754679327124	38.0	35.5	38.0	19.5	38.0
76	33.41207694690991	38.0	34.0	38.0	19.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	1.0
22	7.0
23	21.0
24	65.0
25	126.0
26	256.0
27	473.0
28	778.0
29	1218.0
30	2014.0
31	3150.0
32	4828.0
33	7634.0
34	13047.0
35	25801.0
36	36661.0
37	123395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.60296296296296	13.707122507122508	10.275099715099715	36.41481481481482
2	19.98988499881536	17.663890357032205	37.717563651606554	24.628660992545882
3	19.687346224644152	16.340283220033168	37.51116295175782	26.46120760356486
4	13.85345094680056	25.494359292132167	29.99143414314094	30.660755617926334
5	14.511837285170131	28.054548105487616	40.76254351273032	16.671071096611932
6	28.63957790373435	40.45134775556325	16.42366363520385	14.48541070549855
7	26.97470338442472	25.658841969053565	17.061090962109752	30.30536368441196
8	23.91605460278117	40.58575880734112	15.85913721773679	19.639049372140917
9	22.49038619256775	16.216351673987134	16.087408190417175	45.20585394302794
10-11	23.42852977090889	27.284304434197814	26.722056170150722	22.56510962474257
12-13	23.695757167070656	18.20153456414369	27.905556871821975	30.19715139696368
14-15	21.535156463576882	18.825520785871806	19.69258597751007	39.946736773041245
16-17	29.177677741529827	27.1148098197525	19.08955876724562	24.61795367147205
18-19	29.27586615393027	22.066649656454466	20.552816708888443	28.104667480726818
20-21	22.522052525105252	20.363957790373437	19.973937924875614	37.1400517596457
22-23	22.05503107401265	27.84427454482495	19.899897938726784	30.200796442435617
24-25	29.385713114081852	27.576159362840247	19.693904517163766	23.34422300591414
26-27	29.298875503471905	28.460287229583187	20.060735570176238	22.180101696768666
28-29	21.88075233738541	28.514735096320326	20.43184676228836	29.172665804005902
30-31	21.636534290765276	28.154331225282036	27.887103829120267	22.32203065483242
32-33	27.893027028012174	29.294319196631978	21.29481127777069	21.517842497585157
34-35	22.863775538099837	21.127822632087334	34.41515245402687	21.593249375785962
36-37	21.323971641546226	33.74150248774354	23.200942244254495	21.73358362645574
38-39	21.15857770325685	22.35870892489384	28.087125699393102	28.395587672456212
40-41	20.87358071041936	19.6654759518125	29.468142302575224	29.99280103519291
42-43	28.097669215926153	19.216723506723984	28.95152306293869	23.734084214411176
44-45	21.115292788277536	25.93244819479123	30.729555851209245	22.222703165721995
46-47	27.002496856148277	19.546328527948386	24.602462228216297	28.848712387687037
48-49	26.04681149647342	19.048096375002277	23.75316663325375	31.151925495270554
50-51	20.157817263741222	19.878914100560085	30.87941083985399	29.083857795844708
52-53	18.308048868864898	20.163078528829303	37.48436100609152	24.044511596214278
54-55	18.120003738292194	20.346111150368245	31.487589553752766	30.046295557586795
56-57	25.059782844090446	20.170469440651424	24.4499864363042	30.319761278953933
58-59	18.626307009307457	19.813440659270537	36.76331166759346	24.796940663828547
60-61	25.114520517974594	26.509961575802325	24.570519569904235	23.804998336318846
62-63	18.42261185832722	35.72082932632049	21.64265497454502	24.213903840807266
64-65	18.41190275467657	36.91585590922555	20.571213743762673	24.101027592335207
66-67	18.20094171486916	36.77109400283518	20.086650530797176	24.94131375149849
68-69	18.290640742014933	37.2085128487878	19.805519544875636	24.695326864321633
70-71	18.270700847378457	35.98726767726251	19.93469785797126	25.80733361738778
72-73	18.998539327634965	33.545787862974805	20.445539252436543	27.01013355695369
74-75	19.654990134112275	32.90558368232297	20.674389249144426	26.765036934420326
76	18.985732448085887	33.6966675628506	20.563319951332655	26.754280037730858
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	4.0
12	8.0
13	8.5
14	9.0
15	10.0
16	36.5
17	62.0
18	82.5
19	104.0
20	169.0
21	233.0
22	258.5
23	413.5
24	620.0
25	697.0
26	712.0
27	901.5
28	1270.0
29	1464.0
30	1663.5
31	2151.0
32	2519.5
33	2600.0
34	2941.5
35	3582.0
36	8165.0
37	12449.0
38	14473.0
39	15434.5
40	12562.5
41	9525.5
42	8298.0
43	7861.0
44	7392.5
45	7378.5
46	7396.0
47	7322.0
48	7284.5
49	7552.0
50	7783.0
51	7644.0
52	7443.0
53	7335.5
54	7290.0
55	7318.5
56	7235.5
57	6954.5
58	6785.0
59	6566.0
60	5987.0
61	5213.0
62	4799.0
63	4584.5
64	4133.0
65	3589.5
66	3184.5
67	3086.0
68	2828.5
69	2507.5
70	2343.5
71	2243.0
72	2019.5
73	1678.0
74	1535.5
75	1511.0
76	1415.0
77	1273.0
78	1072.5
79	918.0
80	786.0
81	643.5
82	557.0
83	481.0
84	428.0
85	300.0
86	190.5
87	156.0
88	126.0
89	83.5
90	51.5
91	24.0
92	16.0
93	9.5
94	3.0
95	2.0
96	1.0
97	1.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04601869908327107
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.112613679855655E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	2.2781534199639138E-4
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.020503380779675225
52-53	0.03303322458947675
54-55	0.056726020157101466
56-57	0.06401611110098598
58-59	0.037361716087408195
60-61	0.03781734677140097
62-63	0.031438517195502017
64-65	0.014124551203776266
66-67	0.040551130875357666
68-69	0.008884798337859266
70-71	0.015719258597751008
72-73	0.025743133645592227
74-75	0.015035812571761831
76	0.012302028467805136
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	219476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.13426524995899
#Duplication Level	Percentage of deduplicated	Percentage of total
1	37.993959760091876	12.209079808270609
2	19.264962354842826	12.38130820681988
3	15.829398670012903	15.259982868286281
4	11.534589589802486	14.826222457125152
5	7.2227657492875075	11.60491352129618
6	4.1827952415387015	8.064663106672256
7	2.121173451302338	4.771364522772421
8	0.9499907836715017	2.442180466201316
9	0.45514483814709256	1.3163170460551494
>10	0.408354247309541	1.682644115985347
>50	0.012761070228423156	0.30891760374710675
>100	0.017014760304564207	0.9089832145656017
>500	0.002835793384094035	0.5754615538828847
>1k	0.002835793384094035	2.3546993748747016
>5k	0.0	0.0
>10k+	0.0014178966920470174	11.293262133445115
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT	24786	11.293262133445115	No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG	3440	1.5673695529351728	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	1728	0.7873298219395287	No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT	672	0.30618381964315	RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC	591	0.26927773423973467	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAACCTCGTATGCCGT	279	0.1271209608339864	No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAAACTCGTATGCCGT	261	0.1189196085221163	No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC	260	0.11846397783812353	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.556306839927828E-4	0.0
2	0.0	0.0	0.0	0.0018225227359711313	0.0
3	0.0	0.0	0.0	0.002278153419963914	0.0
4	0.0	0.0	0.0	0.00820135231187009	0.0
5	0.0	0.0	0.0	0.010023875047841222	0.0
6	0.0	0.0	0.0	0.010023875047841222	0.0
7	0.0	0.0	0.0	0.010935136415826788	0.0
8	0.0	0.0	0.0	0.01139076709981957	0.0
9	0.0	0.0	0.0	0.01913648872769688	0.0
10	0.0	0.0	0.0	0.023237164883631922	0.0
11	0.0	0.0	0.0	0.024604056935610272	0.0
12	0.0	0.0	0.0	0.02779347172355975	0.0
13	0.0	0.0	0.0	0.029615994459530882	0.0
14	0.0	0.0	0.0	0.031438517195502017	0.0
15	0.0	0.0	0.0	0.041462392243343235	0.0
16	0.0	0.0	0.0	0.042373653611328804	0.0
17	0.0	0.0	0.0	0.043284914979314366	0.0
18	0.0	0.0	0.0	0.04465180703129271	0.0
19	0.0	0.0	0.0	0.05239752865917002	0.0
20	0.0	0.0	0.0	0.05740946618309063	0.0
21	0.0	0.0	0.0	0.06151014233902568	0.0
22	0.0	0.0	0.0	0.06925586396690299	0.0
23	0.0	0.0	0.0	0.07198964807085968	0.0
24	0.0	0.0	0.0	0.07791284696276586	0.0
25	0.0	0.0	0.0	0.08155789243470812	0.0
26	0.0	0.0	0.0	0.09385992090251326	0.0
27	0.0	0.0	0.0	0.16858335307732963	0.0
28	0.0	0.0	0.0	0.21141263737265123	0.0
29	0.0	0.0	0.0	0.2697333649237274	0.0
30	0.0	0.0	0.0	0.3266872004228253	0.0
31	0.0	0.0	0.0	0.40186626328163444	0.0
32	0.0	0.0	0.0	0.4702108658805519	0.0
33	0.0	0.0	0.0	0.545389928739361	0.0
34	0.0	0.0	0.0	0.6921030089850371	0.0
35	0.0	0.0	0.0	0.8738996518981574	0.0
36	0.0	0.0	0.0	1.1928411306931055	0.0
37	0.0	0.0	0.0	1.7026918660810293	0.0
38	0.0	0.0	0.0	2.284987880223806	0.0
39	0.0	0.0	0.0	2.905101241137983	0.0
40	0.0	0.0	0.0	3.6669157447739162	0.0
41	0.0	0.0	0.0	4.629663380050666	0.0
42	0.0	0.0	0.0	5.802456760648089	0.0
43	0.0	0.0	0.0	6.934699010370155	0.0
44	0.0	0.0	0.0	7.984016475605533	0.0
45	0.0	0.0	0.0	8.93628460515045	0.0
46	0.0	0.0	0.0	9.960086752082232	0.0
47	0.0	0.0	0.0	10.800269733364924	0.0
48	0.0	0.0	0.0	11.542036486905173	0.0
49	0.0	0.0	0.0	12.239151433414133	0.0
50	0.0	0.0	0.0	12.91348484572345	0.0
51	0.0	0.0	0.0	13.708560389290856	0.0
52	0.0	0.0	0.0	14.633035047112212	0.0
53	0.0	0.0	0.0	15.62630993821648	0.0
54	0.0	0.0	0.0	16.44188886256356	0.0
55	0.0	0.0	0.0	17.203703366199495	0.0
56	0.0	0.0	0.0	17.98100931309118	0.0
57	0.0	0.0	0.0	18.695438225591865	0.0
58	0.0	0.0	0.0	19.288669376150466	0.0
59	0.0	0.0	0.0	19.804443310430297	0.0
60	0.0	0.0	0.0	20.283311159306713	0.0
61	0.0	0.0	0.0	20.7954400481146	0.0
62	0.0	0.0	0.0	21.316225919918352	0.0
63	0.0	0.0	0.0	21.903078240901056	0.0
64	0.0	0.0	0.0	22.507700158559476	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	20	0.006960246	52.470097	20
ATGTGCG	20	0.006960246	52.470097	28
TCGGAAA	20	0.006960246	52.470097	29
GTGCGTT	20	0.006960246	52.470097	6
TATGCCG	3515	0.0	50.35537	43
CGTATGC	3540	0.0	50.296192	41
GCCGTCT	3505	0.0	50.279823	46
CTCGTAT	3515	0.0	50.25585	39
TCGTATG	3540	0.0	50.19738	40
ATGCCGT	3515	0.0	50.156334	44
GCTTGAA	3500	0.0	50.151447	56
GTATGCC	3530	0.0	50.14139	42
TCTCGTA	3420	0.0	50.117634	38
CCGTCTT	3520	0.0	50.065567	47
CGTCTTC	3525	0.0	49.89516	48
TGCTTGA	3515	0.0	49.837753	55
TGCCGTC	3535	0.0	49.830383	45
ATCTCGT	3445	0.0	49.753937	37
CTGCTTG	3530	0.0	49.72523	54
TAATCTC	3420	0.0	49.40167	35
>>END_MODULE