##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779808_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	157392
Sequences flagged as poor quality	0
Sequence length	76
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.85015121480126	34.0	23.0	34.0	12.0	34.0
2	29.55509174545085	34.0	27.0	34.0	12.0	34.0
3	29.14640515401037	34.0	27.0	34.0	11.0	34.0
4	29.36627020433059	34.0	27.0	34.0	12.0	34.0
5	29.680250584527805	34.0	31.0	34.0	11.0	34.0
6	32.31335137745248	37.0	31.0	38.0	12.0	38.0
7	32.617318542238486	37.0	31.0	38.0	12.0	38.0
8	31.938275134695537	37.0	28.0	38.0	11.0	38.0
9	32.737191216834404	37.0	33.0	38.0	12.0	38.0
10-11	31.361771881671242	37.0	28.5	38.0	11.5	38.0
12-13	32.19884428687608	37.0	31.0	38.0	11.0	38.0
14-15	31.002010902714243	37.0	26.0	38.0	11.0	38.0
16-17	31.625298617464676	37.0	28.5	38.0	11.0	38.0
18-19	32.41685727355902	37.5	31.0	38.0	11.0	38.0
20-21	31.231641379485616	37.0	27.0	38.0	11.0	38.0
22-23	32.08276468943784	37.0	29.5	38.0	11.0	38.0
24-25	32.36174329063739	37.5	31.0	38.0	11.0	38.0
26-27	31.304608874656907	37.0	26.5	38.0	11.0	38.0
28-29	32.41267662905357	37.5	32.0	38.0	11.0	38.0
30-31	32.762160719731625	38.0	34.0	38.0	11.0	38.0
32-33	32.89318072074819	38.0	34.0	38.0	11.0	38.0
34-35	32.85482108366372	38.0	34.0	38.0	11.0	38.0
36-37	32.37953009047474	38.0	31.5	38.0	11.0	38.0
38-39	32.313716707329476	37.5	32.0	38.0	11.0	38.0
40-41	32.309348632713224	37.5	31.0	38.0	11.0	38.0
42-43	32.655125419335164	38.0	32.5	38.0	11.0	38.0
44-45	32.72889981701738	38.0	33.5	38.0	11.0	38.0
46-47	32.62819584222832	38.0	33.0	38.0	11.0	38.0
48-49	32.32716084680288	37.5	31.5	38.0	11.0	38.0
50-51	31.75076560435092	37.5	28.5	38.0	11.0	38.0
52-53	32.29040866117719	37.0	31.0	38.0	11.0	38.0
54-55	32.411764892751854	37.5	32.0	38.0	11.0	38.0
56-57	32.47259390566229	38.0	31.5	38.0	11.0	38.0
58-59	32.65906145166209	38.0	33.5	38.0	11.0	38.0
60-61	32.58261855748704	38.0	32.5	38.0	11.0	38.0
62-63	32.542749949171494	38.0	32.0	38.0	11.0	38.0
64-65	32.57189691979262	38.0	32.5	38.0	11.0	38.0
66-67	32.48698154925282	38.0	31.0	38.0	11.0	38.0
68-69	31.918874529836334	37.0	29.0	38.0	11.0	38.0
70-71	31.969496543661684	37.0	30.0	38.0	11.0	38.0
72-73	32.12341478601199	37.0	31.0	38.0	11.0	38.0
74-75	32.11414493748094	37.0	30.5	38.0	11.0	38.0
76	31.149880553014132	37.0	27.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	36.0
13	381.0
14	1488.0
15	2822.0
16	3841.0
17	4332.0
18	4393.0
19	3896.0
20	3130.0
21	2471.0
22	1940.0
23	1643.0
24	1520.0
25	1499.0
26	1601.0
27	1857.0
28	2160.0
29	2574.0
30	3144.0
31	3867.0
32	4955.0
33	6540.0
34	8964.0
35	13558.0
36	24205.0
37	50574.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.75810888943954	17.92564918768148	9.45555280229241	33.86068912058657
2	20.537257293890413	20.568389752973466	32.001626512148015	26.892726440988106
3	18.151494358035986	19.99529836332215	33.30728372471282	28.545923553929043
4	13.286571109077972	24.103512249669613	29.374428179322965	33.23548846192945
5	17.366829317881468	31.075912371658028	32.75071159906476	18.80654671139575
6	27.212193990685503	36.19122047919486	15.900094669894338	20.696490860225303
7	26.273151825142165	31.417225275598053	18.109095530069574	24.2005273691902
8	23.072426566361898	38.539396519407575	16.43274222140329	21.955434692827236
9	24.112186350321828	18.995304388712743	17.68193110985443	39.210578151110994
10-11	23.32024474391476	26.19401610023572	23.220491902229508	27.26524725362001
12-13	23.558376537562264	22.685396970621124	24.627998881772896	29.128227610043712
14-15	25.207286312432096	24.203416967933364	21.445635392112635	29.1436613275219
16-17	25.943586534215633	25.24310397956688	21.847749084289813	26.96556040192768
18-19	23.010013469896055	25.17282130785066	23.964025211579028	27.85314001067426
20-21	24.793191521805426	24.133056317983126	23.84333384161838	27.23041831859307
22-23	26.229747760340555	23.929411017218374	22.647563377597052	27.193277844844015
24-25	23.045838800166468	27.319022673194038	21.93044599827815	27.704692528361345
26-27	24.02861653901259	27.32326713958505	23.24617276028248	25.401943561119882
28-29	23.10769123239537	26.752043614043668	24.446167091856996	25.694098061703958
30-31	23.80409299134004	26.171127955219802	24.22628994033966	25.79848911310049
32-33	22.25660216720725	27.33279751447823	24.27322949460422	26.137370823710306
34-35	22.07432022441569	27.99763639193959	24.08338707576571	25.84465630787901
36-37	24.50751124780763	24.949162451386595	24.35976462214992	26.183561678655852
38-39	24.466415468391588	24.50549310899167	25.613328334784185	25.414763087832558
40-41	22.54918467642789	25.992509125333964	25.088076547971145	26.370229650267007
42-43	21.042883782693362	25.184175664986135	26.076542103875394	27.69639844844511
44-45	20.759284556978113	23.339263589287416	27.81808939860851	28.083362455125965
46-47	21.603504747865923	24.636484069472285	26.84791897653229	26.912092206129497
48-49	21.665083565503853	25.011833548825702	26.487770940616233	26.83531194505421
50-51	23.577401072508707	24.915177777213003	25.027956388034667	26.479464762243627
52-53	19.142811755361397	26.704368546465453	26.20810166799047	27.944718030182685
54-55	19.974329974202895	25.119772273830556	25.87652971750264	29.029368034463914
56-57	20.54114679146849	25.8992371456985	25.108740893248054	28.45087516958496
58-59	20.4331788157322	25.654899394108842	25.489685235255433	28.422236554903524
60-61	19.5928340832253	25.10358677139734	25.65297796080226	29.650601184575105
62-63	20.42840621703712	25.346308793066196	24.970770267007257	29.254514722889425
64-65	21.508577820004003	24.536155852904447	24.301969768705494	29.653296558386057
66-67	20.2618802506323	27.29026067284351	22.88989717975115	29.557961896773044
68-69	20.727930034341117	28.25374940832256	22.271533087873234	28.746787469463094
70-71	21.461519400728125	28.602651994078442	21.768716127351627	28.167112477841812
72-73	22.029132845138435	28.70681301923657	21.483646529950914	27.780407605674075
74-75	23.12829424977681	28.662475020095123	21.415522647917573	26.793708082210493
76	22.40167736196709	28.9802401677362	21.859076180189337	26.75900629010738
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	1.5
11	2.0
12	1.0
13	0.5
14	2.5
15	9.5
16	17.0
17	20.0
18	23.5
19	31.0
20	40.5
21	46.0
22	76.0
23	131.5
24	218.0
25	279.0
26	319.0
27	474.5
28	667.5
29	745.0
30	845.0
31	1062.5
32	1456.0
33	1732.0
34	2036.0
35	2667.0
36	3376.0
37	3758.0
38	4249.5
39	5129.5
40	6077.5
41	6994.5
42	7352.0
43	7642.5
44	8376.5
45	8798.5
46	8777.0
47	8855.5
48	8874.5
49	8684.5
50	8554.0
51	8157.5
52	7416.0
53	6640.5
54	6210.0
55	5942.0
56	5345.5
57	4679.0
58	4341.0
59	4076.0
60	3552.5
61	3065.5
62	2837.0
63	2678.5
64	2359.0
65	2098.0
66	1917.0
67	1836.0
68	1744.0
69	1546.0
70	1330.0
71	1220.0
72	1164.0
73	1088.0
74	969.0
75	870.0
76	822.5
77	735.5
78	617.5
79	539.0
80	523.0
81	453.5
82	352.0
83	304.0
84	276.5
85	199.5
86	132.0
87	114.0
88	103.5
89	81.5
90	55.5
91	38.0
92	35.0
93	27.5
94	17.5
95	15.5
96	16.0
97	12.5
98	9.0
99	8.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001906068923452272
2	0.0
3	0.0
4	0.0
5	0.0
6	0.001906068923452272
7	0.0044474941547219685
8	0.00317678153908712
9	0.006988919385991664
10-11	0.001906068923452272
12-13	0.0
14-15	6.35356307817424E-4
16-17	3.17678153908712E-4
18-19	0.002541425231269696
20-21	0.0
22-23	0.001270712615634848
24-25	0.0022237470773609842
26-27	3.17678153908712E-4
28-29	0.007306597539900376
30-31	6.35356307817424E-4
32-33	3.17678153908712E-4
34-35	0.003494459692995832
36-37	0.017789976618887874
38-39	0.008259632001626513
40-41	3.17678153908712E-4
42-43	9.53034461726136E-4
44-45	0.0044474941547219685
46-47	0.003494459692995832
48-49	3.17678153908712E-4
50-51	0.002541425231269696
52-53	0.010801057232896209
54-55	0.00635356307817424
56-57	0.014295516925892041
58-59	0.013024804310257192
60-61	0.022872827081427266
62-63	0.011436413540713633
64-65	0.024461217850970822
66-67	0.01906068923452272
68-69	3.17678153908712E-4
70-71	6.35356307817424E-4
72-73	0.006035884924265529
74-75	0.009848022771170072
76	0.001270712615634848
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	157392.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.8572340521874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70503087533682	52.6200067031703
2	9.417170257913726	12.592119120852718
3	5.412045430019545	10.855031640476637
4	2.9672913559260183	7.935395707367131
5	1.53046087633612	5.11611905084599
6	0.7302587776666248	2.9293849210273093
7	0.4375554630841578	2.0477623604361543
8	0.22976654558171944	1.2289244572255678
9	0.14185586591685254	0.853568174635085
>10	0.42856455221841094	3.821687863963107
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00317678153908712	0.0
2	0.0	0.0	0.0	0.003812137846904544	0.0
3	0.0	0.0	0.0	0.0057182067703568165	0.0
4	0.0	0.0	0.0	0.00635356307817424	0.0
5	0.0	0.0	0.0	0.008259632001626513	0.0
6	0.0	0.0	0.0	0.008259632001626513	0.0
7	0.0	0.0	0.0	0.008259632001626513	0.0
8	0.0	0.0	0.0	0.008259632001626513	0.0
9	0.0	0.0	0.0	0.01270712615634848	0.0
10	0.0	0.0	0.0	0.013342482464165905	0.0
11	0.0	0.0	0.0	0.013342482464165905	0.0
12	0.0	0.0	0.0	0.016519264003253025	0.0
13	0.0	0.0	0.0	0.01715462031107045	0.0
14	0.0	0.0	0.0	0.02033140185015757	0.0
15	0.0	0.0	0.0	0.020966758157974993	0.0
16	0.0	0.0	0.0	0.020966758157974993	0.0
17	0.0	0.0	0.0	0.020966758157974993	0.0
18	0.0	0.0	0.0	0.021602114465792417	0.0
19	0.0	0.0	0.0	0.02541425231269696	0.0
20	0.0	0.0	0.0	0.03494459692995832	0.0
21	0.0	0.0	0.0	0.03812137846904544	0.0
22	0.0	0.0	0.0	0.04892243570194165	0.0
23	0.0	0.0	0.0	0.0501931483175765	0.0
24	0.0	0.0	0.0	0.060994205550472705	0.0
25	0.0	0.0	0.0	0.06417098708955983	0.0
26	0.0	0.0	0.0	0.0686184812442818	0.0
27	0.0	0.0	0.0	0.10673985971332724	0.0
28	0.0	0.0	0.0	0.1429551692589204	0.0
29	0.0	0.0	0.0	0.18361797295923554	0.0
30	0.0	0.0	0.0	0.23126969604554234	0.0
31	0.0	0.0	0.0	0.2852749822100234	0.0
32	0.0	0.0	0.0	0.33165599268069534	0.0
33	0.0	0.0	0.0	0.3964623360780726	0.0
34	0.0	0.0	0.0	0.47778794347870285	0.0
35	0.0	0.0	0.0	0.6112127681203618	0.0
36	0.0	0.0	0.0	0.8304106943173731	0.0
37	0.0	0.0	0.0	1.2128951916234625	0.0
38	0.0	0.0	0.0	1.63032428585951	0.0
39	0.0	0.0	0.0	2.172283216427773	0.0
40	0.0	0.0	0.0	2.833689132865711	0.0
41	0.0	0.0	0.0	3.61581274778896	0.0
42	0.0	0.0	0.0	4.4614719934939515	0.0
43	0.0	0.0	0.0	5.38909220290739	0.0
44	0.0	0.0	0.0	6.184558300294805	0.0
45	0.0	0.0	0.0	7.053725729389042	0.0
46	0.0	0.0	0.0	7.851097895699908	0.0
47	0.0	0.0	0.0	8.570321236149233	0.0
48	0.0	0.0	0.0	9.31368811629562	0.0
49	0.0	0.0	0.0	10.00368506658534	0.0
50	0.0	0.0	0.0	10.7387923147301	0.0
51	0.0	0.0	0.0	11.513291653959541	0.0
52	0.0	0.0	0.0	12.338619497814374	0.0
53	0.0	0.0	0.0	13.101047067195283	0.0
54	0.0	0.0	0.0	13.8418725221104	0.0
55	0.0	0.0	0.0	14.573167632408255	0.0
56	0.0	0.0	0.0	15.271424214699604	0.0
57	0.0	0.0	0.0	15.966504015451866	0.0
58	0.0	0.0	0.0	16.595506760191114	0.0
59	0.0	0.0	0.0	17.13428890922029	0.0
60	0.0	0.0	0.0	17.686413540713634	0.0
61	0.0	0.0	0.0	18.239808884822608	0.0
62	0.0	0.0	0.0	18.826878113245908	0.0
63	0.0	0.0	0.0	19.43872623767409	0.0
64	0.0	0.0	0.0	20.06899969502897	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTGGA	20	7.907404E-5	70.0	46
ACGAGTG	20	7.907404E-5	70.0	24
AATGGCG	20	0.006940948	52.5	70
ATGGTTC	20	0.006940948	52.5	9
GACGATT	20	0.006940948	52.5	23
GGTTCTA	20	0.006940948	52.5	32
TCGAAAG	20	0.006940948	52.5	3
ACGTGTA	30	5.86386E-4	46.666668	34
CGACGAT	80	2.910383E-11	43.75	22
TCTCGGT	100	0.0	42.0	43
TAGATCT	150	0.0	41.999996	39
TCGGTGG	85	5.638867E-11	41.17647	45
TGGTCGC	85	5.638867E-11	41.17647	49
CGCCGTA	60	2.580764E-7	40.833336	53
AATATGC	35	0.0012524688	40.000004	46
GTTACAT	35	0.0012524688	40.000004	3
CGGTGGT	70	1.9101208E-8	40.000004	46
GACGATC	35	0.0012524688	40.000004	23
CTCGGTG	80	1.4078978E-9	39.375	44
GTAGATC	180	0.0	38.888885	38
>>END_MODULE