FastQCFastQC Report
Wed 25 May 2016
SRR1779808_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779808_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157392
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1980312.581960963708447No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG25541.6227000101657008No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC13570.8621785097082444No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT5400.34309240622140896Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC4120.2617667988207787No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGAACTCGTATGCCGT3340.21220900681101965No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGACCTCGTATGCCGT3310.21030293788756738No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1890.12008234217749314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA150.002219167370.01716648
TCCGAAA150.002221967669.9949236
TCGGAGT150.002221967669.9949218
TCACGTA150.002221967669.9949225
CCTAGAT252.3855337E-455.9959343
ACGTAGG252.3855337E-455.99593427
ATCTCGT27450.055.4604537
TCTCGTA27500.055.2323538
GCCGTCT28900.055.220946
GATCTCG27400.055.17847436
TGCCGTC28950.055.12553445
CCGTCTT28850.055.0739947
AGATCTC27350.055.02342635
CTCGTAT28750.055.02209539
TATGCCG29100.054.8413843
ATGCCGT29050.054.81529644
CGTATGC29100.054.72111541
TCGTATG29100.054.72111540
GTCTTCT29050.054.59169849
GTATGCC29250.054.5601442