FastQCFastQC Report
Wed 25 May 2016
SRR1779807_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779807_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72676
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT150.002216501670.02
TCGATGA150.002216501670.018
TGCCGCG150.002216501670.019
CTCGGTG453.765308E-1062.22222544
ACGATCT308.301671E-658.33333224
AGTGTAG850.057.6470635
GCGGTGA252.3761067E-455.99999620
TCGGCGG252.3761067E-455.99999645
CAGTGTA252.3761067E-455.99999634
CGGTCGT252.3761067E-455.99999610
CCTTGTG252.3761067E-455.99999632
TCGGTGG700.055.00000445
ATCTCGG653.6379788E-1253.84615342
GGAGCGG200.006925983452.54
TTAGCGA200.006925983452.515
GGGTCGC200.006925983452.549
TTCACCG200.006925983452.562
GGTGGTC407.3868614E-752.547
GGCGGTC200.006925983452.547
CGGTGGT407.3868614E-752.546
Produced by FastQC (version 0.11.5)