##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779807_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	72676
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.615746601353955	32.0	21.0	34.0	12.0	34.0
2	26.70701469536023	32.0	21.0	34.0	12.0	34.0
3	26.28005118608619	32.0	21.0	34.0	11.0	34.0
4	26.40878694479608	32.0	21.0	34.0	12.0	34.0
5	26.456588144641973	33.0	21.0	34.0	11.0	34.0
6	29.379932852661128	35.0	21.0	38.0	11.0	38.0
7	29.508420936760416	36.0	21.0	38.0	12.0	38.0
8	28.78373878584402	35.0	21.0	38.0	11.0	38.0
9	29.65347570036876	36.0	21.0	38.0	11.0	38.0
10-11	28.513085475260056	34.0	21.0	38.0	11.0	38.0
12-13	29.065076503935273	35.0	21.0	38.0	11.0	38.0
14-15	28.041464857724694	34.0	21.0	38.0	11.0	38.0
16-17	28.43239859100666	34.5	21.0	38.0	11.0	38.0
18-19	28.836390280147505	35.0	21.0	38.0	11.0	38.0
20-21	28.240746601353955	33.0	21.0	38.0	11.0	38.0
22-23	28.75809070394628	35.0	21.0	38.0	11.0	38.0
24-25	28.980495624415212	35.0	21.0	38.0	11.0	38.0
26-27	28.079537949254224	34.0	21.0	38.0	11.0	38.0
28-29	28.76829352193296	35.0	21.0	38.0	11.0	38.0
30-31	29.048097033408553	36.5	21.0	38.0	11.0	38.0
32-33	29.228954537949257	36.5	21.0	38.0	11.0	38.0
34-35	29.288348285541307	36.5	21.0	38.0	11.0	38.0
36-37	28.90897270075403	35.5	21.0	38.0	11.0	38.0
38-39	28.771430733667238	35.5	21.0	38.0	11.0	38.0
40-41	28.730296108756672	35.5	21.0	38.0	11.0	38.0
42-43	29.087924486763168	36.5	21.0	38.0	11.0	38.0
44-45	29.173846937090648	36.0	21.0	38.0	11.0	38.0
46-47	29.054330177775334	36.0	21.0	38.0	11.0	38.0
48-49	28.792448676316802	35.5	21.0	38.0	11.0	38.0
50-51	28.33940365457648	35.0	18.0	38.0	11.0	38.0
52-53	28.798097033408553	35.0	21.0	38.0	11.0	38.0
54-55	28.847914029390722	35.5	21.0	38.0	11.0	38.0
56-57	28.801433760801366	36.0	21.0	38.0	11.0	38.0
58-59	28.962208982332545	36.0	21.0	38.0	11.0	38.0
60-61	28.991317628928396	36.0	21.0	38.0	11.0	38.0
62-63	28.86490725961803	36.0	21.0	38.0	11.0	38.0
64-65	28.89838461115086	36.0	21.0	38.0	11.0	38.0
66-67	29.000165116407068	36.0	21.0	38.0	11.0	38.0
68-69	28.46519483736034	34.0	21.0	38.0	11.0	38.0
70-71	28.582351808024658	34.0	21.0	38.0	11.0	38.0
72-73	28.557529308162252	35.0	20.0	38.0	11.0	38.0
74-75	28.392509218999393	35.0	19.0	38.0	11.0	38.0
76	27.68938851890583	34.0	17.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	29.0
13	401.0
14	1511.0
15	2974.0
16	3962.0
17	4466.0
18	4173.0
19	3538.0
20	2752.0
21	1920.0
22	1303.0
23	968.0
24	756.0
25	691.0
26	722.0
27	676.0
28	831.0
29	865.0
30	1084.0
31	1328.0
32	1686.0
33	2265.0
34	3191.0
35	4694.0
36	8583.0
37	17305.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.698937751114535	20.499201937365843	9.982662777257968	39.819197534261654
2	14.411910396829766	20.195112554350818	31.061148109417143	34.33182893940228
3	20.891353404149925	20.528097308602565	31.717485827508398	26.863063459739116
4	18.759975782926965	25.67009741868017	26.065000825582036	29.50492597281083
5	11.827838626231493	29.591612086520996	29.949364301832794	28.631184985414716
6	21.822710259797446	44.22198370761779	13.859533245266404	20.09577278731836
7	20.485475636773952	35.53824771917271	16.49901611371799	27.477260530335347
8	18.466280461794614	39.29932712286544	19.667551910613295	22.566840504726652
9	23.22691619650475	18.6046511627907	17.20379799091785	40.96463464978671
10-11	19.943721663031738	29.215199278976804	23.172502046797707	27.668577011193747
12-13	19.179646650888877	22.99933953437173	26.037481424404206	31.783532390335186
14-15	25.553827948703837	25.696240849799107	21.220898233254445	27.52903296824261
16-17	27.708099703476414	24.977468335271173	20.106500815267868	27.20793114598455
18-19	21.877536979704164	24.399724802201582	23.491572067423462	30.231166150670795
20-21	24.93188948208487	23.860008806208377	22.526693819142494	28.68140789256426
22-23	28.187910228007652	23.378008338722772	20.36106944807563	28.07301198519395
24-25	21.77394941794865	29.915788314940695	20.12617992679638	28.18408234031428
26-27	23.674940833287465	30.049810116131876	21.89993945731741	24.37530959326325
28-29	22.237818051713937	28.14886677950708	24.437518061346655	25.17579710743233
30-31	23.506384501073256	26.4193131157466	25.10663767956409	24.967664703616048
32-33	19.723154824151027	29.299906434035993	24.785348670812922	26.191590071000054
34-35	19.32603131794039	32.46759501334728	23.02061259872857	25.185761069983766
36-37	24.464490913719906	25.592276833942297	23.409642948069553	26.533589304268247
38-39	25.085662386987572	23.638690500763737	26.284935804813607	24.99071130743508
40-41	20.961672090319297	27.104732681577698	25.282935789915445	26.650659438187557
42-43	17.851835544058563	26.497743409103418	26.370466178655956	29.279954868182067
44-45	17.646856466638226	23.63188529440095	30.114347832069683	28.606910406891146
46-47	19.313514368863906	26.399218432875355	27.5179051799462	26.769362018314542
48-49	19.7032032582971	27.731300566899662	25.21602729924597	27.34946887555727
50-51	23.987423201029262	27.443978891893195	21.85941230297151	26.709185604106032
52-53	16.53800107336003	29.873123340076233	24.750581403349432	28.838294183214302
54-55	17.724279807077053	25.99093179582092	25.39028367379234	30.89450472330969
56-57	19.881511859135355	27.92797132064047	23.352897867626314	28.837618952597865
58-59	19.503904771734266	27.49165720576599	23.251109505624935	29.753328516874806
60-61	17.239338531308277	25.542793831247035	24.782366340244852	32.435501297199835
62-63	19.529379386266683	26.16691894867208	23.172560891702215	31.13114077335902
64-65	19.918388692774027	25.65113574589019	22.786482525133668	31.64399303620212
66-67	17.814478392513074	31.225571153316817	20.514726121662537	30.44522433250757
68-69	19.24569321371567	33.098959766635474	19.50850349496395	28.1468435246849
70-71	19.478232153668337	34.30637349331278	18.773047498486434	27.442346854532445
72-73	20.620024905572105	33.33218666795093	19.25985042896752	26.78793799750944
74-75	22.540938489060135	32.61593504885097	18.53997523049401	26.303151231594878
76	20.112554350817327	32.87742858715394	20.336837470416643	26.673179591612083
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	1.0
14	2.0
15	6.0
16	15.0
17	21.0
18	21.5
19	22.0
20	28.5
21	35.0
22	48.5
23	84.0
24	137.5
25	169.0
26	205.0
27	261.5
28	339.5
29	397.0
30	429.5
31	532.0
32	685.0
33	768.0
34	908.0
35	1188.5
36	1474.0
37	1619.0
38	1870.0
39	2343.0
40	2740.5
41	3095.0
42	3274.0
43	3393.5
44	3772.5
45	4129.5
46	4227.0
47	4304.0
48	4352.0
49	4240.5
50	4158.0
51	4081.5
52	3852.5
53	3475.0
54	3250.0
55	3055.5
56	2720.5
57	2414.5
58	2249.0
59	2123.5
60	1771.5
61	1454.0
62	1363.0
63	1282.5
64	1080.5
65	871.0
66	688.0
67	593.0
68	580.0
69	510.0
70	407.5
71	362.0
72	353.5
73	280.0
74	196.0
75	177.0
76	163.0
77	148.0
78	124.0
79	101.0
80	97.5
81	81.5
82	56.0
83	43.0
84	46.5
85	38.5
86	21.5
87	16.0
88	15.0
89	14.5
90	9.5
91	6.0
92	8.0
93	5.5
94	4.5
95	3.5
96	1.0
97	1.5
98	4.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0055038802355660745
7	0.006879850294457592
8	0.0041279101766745555
9	0.008255820353349111
10-11	0.0020639550883372777
12-13	0.0
14-15	0.0
16-17	6.879850294457593E-4
18-19	0.0013759700588915186
20-21	0.0
22-23	0.0041279101766745555
24-25	0.0027519401177830373
26-27	0.0
28-29	0.00963179041224063
30-31	0.0
32-33	0.0
34-35	0.0027519401177830373
36-37	0.01582365567725246
38-39	0.00963179041224063
40-41	6.879850294457593E-4
42-43	0.0
44-45	0.0041279101766745555
46-47	0.0020639550883372777
48-49	0.0
50-51	0.003439925147228796
52-53	0.00963179041224063
54-55	0.006191865265011833
56-57	0.014447685618360944
58-59	0.011695745500577908
60-61	0.02683141614838461
62-63	0.008255820353349111
64-65	0.01995156585392702
66-67	0.022015520942264298
68-69	0.0
70-71	0.0
72-73	0.0020639550883372777
74-75	0.008255820353349111
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	72676.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.15234740492046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.04707912250431	68.02933568165557
2	6.005493151167294	9.38686774175794
3	3.304693827247438	7.7480874016181405
4	1.7394978696432972	5.4378336727392815
5	0.8591851825768514	3.357366943695305
6	0.4648050987710835	2.179536573284165
7	0.23768442550794042	1.300291705652485
8	0.15493503292369448	0.968682921459629
9	0.0669037642170499	0.4705817601408993
>10	0.11972252544103666	1.1214155979965876
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01788761076558974	0.0
2	0.0	0.0	0.0	0.01788761076558974	0.0
3	0.0	0.0	0.0	0.01788761076558974	0.0
4	0.0	0.0	0.0	0.01926358082448126	0.0
5	0.0	0.0	0.0	0.02751940117783037	0.0
6	0.0	0.0	0.0	0.02751940117783037	0.0
7	0.0	0.0	0.0	0.02751940117783037	0.0
8	0.0	0.0	0.0	0.028895371236721888	0.0
9	0.0	0.0	0.0	0.028895371236721888	0.0
10	0.0	0.0	0.0	0.03164731135450492	0.0
11	0.0	0.0	0.0	0.034399251472287964	0.0
12	0.0	0.0	0.0	0.03577522153117948	0.0
13	0.0	0.0	0.0	0.03577522153117948	0.0
14	0.0	0.0	0.0	0.037151191590071	0.0
15	0.0	0.0	0.0	0.03852716164896252	0.0
16	0.0	0.0	0.0	0.03852716164896252	0.0
17	0.0	0.0	0.0	0.03852716164896252	0.0
18	0.0	0.0	0.0	0.041279101766745555	0.0
19	0.0	0.0	0.0	0.04540701194342011	0.0
20	0.0	0.0	0.0	0.050910892178986186	0.0
21	0.0	0.0	0.0	0.06742253288568441	0.0
22	0.0	0.0	0.0	0.08668611371016567	0.0
23	0.0	0.0	0.0	0.0963179041224063	0.0
24	0.0	0.0	0.0	0.11970939512356212	0.0
25	0.0	0.0	0.0	0.12521327535912818	0.0
26	0.0	0.0	0.0	0.12521327535912818	0.0
27	0.0	0.0	0.0	0.13484506577136882	0.0
28	0.0	0.0	0.0	0.15135670647806704	0.0
29	0.0	0.0	0.0	0.18713192800924652	0.0
30	0.0	0.0	0.0	0.21602729924596842	0.0
31	0.0	0.0	0.0	0.24354670042379878	0.0
32	0.0	0.0	0.0	0.2862017722494358	0.0
33	0.0	0.0	0.0	0.31784908360394076	0.0
34	0.0	0.0013759700588915184	0.0	0.39765534701964883	0.0
35	0.0	0.0013759700588915184	0.0	0.4733337002586824	0.0
36	0.0	0.0013759700588915184	0.0	0.6384501073256645	0.0
37	0.0	0.0013759700588915184	0.0	0.9246518795751004	0.0
38	0.0	0.0013759700588915184	0.0	1.2149815620012108	0.0
39	0.0	0.0013759700588915184	0.0	1.64015631019869	0.0
40	0.0	0.0013759700588915184	0.0	2.0240519566294237	0.0
41	0.0	0.0013759700588915184	0.0	2.4987616269469974	0.0
42	0.0	0.0013759700588915184	0.0	3.038141890032473	0.0
43	0.0	0.0013759700588915184	0.0	3.5114755902911554	0.0
44	0.0	0.0013759700588915184	0.0	4.05085585337663	0.0
45	0.0	0.0013759700588915184	0.0	4.5503329847542515	0.0
46	0.0	0.0013759700588915184	0.0	5.150255930430954	0.0
47	0.0	0.0013759700588915184	0.0	5.630469480984094	0.0
48	0.0	0.0013759700588915184	0.0	6.113434971655017	0.0
49	0.0	0.0013759700588915184	0.0	6.573008971324784	0.0
50	0.0	0.0013759700588915184	0.0	7.1165171445869335	0.0
51	0.0	0.0013759700588915184	0.0	7.53893995266663	0.0
52	0.0	0.0013759700588915184	0.0	8.065936485222082	0.0
53	0.0	0.0013759700588915184	0.0	8.564037646540811	0.0
54	0.0	0.0013759700588915184	0.0	9.026363586328362	0.0
55	0.0	0.0013759700588915184	0.0	9.513456987175958	0.0
56	0.0	0.0013759700588915184	0.0	10.01155814849469	0.0
57	0.0	0.0013759700588915184	0.0	10.377566184159832	0.0
58	0.0	0.0013759700588915184	0.0	10.790357201827288	0.0
59	0.0	0.0013759700588915184	0.0	11.203148219494743	0.0
60	0.0	0.0013759700588915184	0.0	11.648962518575596	0.0
61	0.0	0.0013759700588915184	0.0	11.992955033298475	0.0
62	0.0	0.0013759700588915184	0.0	12.441521272497111	0.0
63	0.0	0.0013759700588915184	0.0	12.822664978810062	0.0
64	0.0	0.0013759700588915184	0.0	13.133634212119544	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCAAT	15	0.0022165016	70.0	2
TCGATGA	15	0.0022165016	70.0	18
TGCCGCG	15	0.0022165016	70.0	19
CTCGGTG	45	3.765308E-10	62.222225	44
ACGATCT	30	8.301671E-6	58.333332	24
AGTGTAG	85	0.0	57.64706	35
GCGGTGA	25	2.3761067E-4	55.999996	20
TCGGCGG	25	2.3761067E-4	55.999996	45
CAGTGTA	25	2.3761067E-4	55.999996	34
CGGTCGT	25	2.3761067E-4	55.999996	10
CCTTGTG	25	2.3761067E-4	55.999996	32
TCGGTGG	70	0.0	55.000004	45
ATCTCGG	65	3.6379788E-12	53.846153	42
GGAGCGG	20	0.0069259834	52.5	4
TTAGCGA	20	0.0069259834	52.5	15
GGGTCGC	20	0.0069259834	52.5	49
TTCACCG	20	0.0069259834	52.5	62
GGTGGTC	40	7.3868614E-7	52.5	47
GGCGGTC	20	0.0069259834	52.5	47
CGGTGGT	40	7.3868614E-7	52.5	46
>>END_MODULE