FastQCFastQC Report
Wed 25 May 2016
SRR1779807_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779807_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72676
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT1917726.38697781936265No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG24403.357366943695305No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC14291.9662612141559799No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT4690.6453299576201222RNA PCR Primer, Index 30 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCGT3930.5407562331443668No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC3790.5214926523198855No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAACTCGTATGCCGT3480.4788375804942484No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGAGCTCGTATGCCGT1770.24354670042379878No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC1730.23804282018823272No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATATCGTATGCCGT1560.21465132918707688No Hit
CTTTACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC1380.18988386812702956No Hit
CTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1140.15686058671363312No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGACCTCGTATGCCG1110.15273267653695854No Hit
CCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG800.11007760471132147No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCG150.002216588269.9993137
GGTCGCT150.002216588269.9993139
CCGATCT150.002216588269.9993134
GGGTCGC150.002216588269.9993138
GACGTAG150.002216588269.9993118
GGCAAGA150.002216588269.9993132
GCAAAAA207.879192E-569.9993160
TGACGTA150.002216588269.9993117
GCTTGAC455.456968E-1269.9993156
GTAGCGC150.002216588269.9993121
CGTAGCG150.002216588269.9993120
TAACAGT150.002216588269.999316
CTGCCGT252.8144404E-669.9993144
GCGGGTC150.002216588269.9993136
AGCGCTT150.002216588269.9993123
CTTGACA401.3278623E-1069.9993157
GTCCGTC150.002216588269.9993170
CGTAAGC150.002216588269.9993162
CAAGAGT150.002216588269.999316
TTGCCCG150.002216588269.9993130
Produced by FastQC (version 0.11.5)