FastQCFastQC Report
Wed 25 May 2016
SRR1779806_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779806_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156758
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGTGC150.00222131270.028
AGTCCAT352.9592957E-760.00000421
TAGTCCA308.346518E-658.33333220
TGGTCGC553.45608E-1157.2727349
TCGCCGT252.3846503E-455.99999652
CTTCCGA452.683555E-854.44444718
GATCGCT200.006940898552.543
CGAATTT200.006940898552.518
GGCGGTC200.006940898552.547
GGCATGC200.006940898552.525
ATTGCGG200.006940898552.53
TCGAATT200.006940898552.517
GCCGTAT200.006940898552.554
AGGTCCA200.006940898552.511
ATCGTCA352.0791555E-550.0000047
TTACGCT352.0791555E-550.00000413
GATCTCG850.049.41176641
TTTACGG305.8637926E-446.66666436
TACGGAC305.8637926E-446.66666438
GTAGATC1000.045.49999638