##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779806_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156758
Sequences flagged as poor quality	0
Sequence length	76
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.54468671455364	34.0	23.0	34.0	12.0	34.0
2	29.112364281248805	34.0	24.0	34.0	12.0	34.0
3	28.82950790390283	34.0	23.0	34.0	11.0	34.0
4	29.153548782199312	34.0	24.0	34.0	12.0	34.0
5	29.541107949833503	34.0	31.0	34.0	11.0	34.0
6	32.20520164840072	37.0	31.0	38.0	12.0	38.0
7	32.13541892598783	37.0	28.0	38.0	12.0	38.0
8	31.465437170670715	37.0	27.0	38.0	11.0	38.0
9	32.39329412215007	37.0	31.0	38.0	12.0	38.0
10-11	31.09707319562638	37.0	26.0	38.0	11.0	38.0
12-13	31.86592709781957	37.0	27.5	38.0	11.0	38.0
14-15	30.70025134283418	37.0	25.5	38.0	11.0	38.0
16-17	31.277092716161217	37.0	26.5	38.0	11.0	38.0
18-19	32.01239809132548	37.0	31.0	38.0	11.0	38.0
20-21	30.925152145345052	36.5	25.5	38.0	11.0	38.0
22-23	31.707488613021347	37.0	28.5	38.0	11.0	38.0
24-25	32.013252912132074	37.0	29.5	38.0	11.0	38.0
26-27	30.95793835083377	37.0	25.0	38.0	11.0	38.0
28-29	32.016975210196605	37.5	29.0	38.0	11.0	38.0
30-31	32.344409854680464	38.0	31.0	38.0	11.0	38.0
32-33	32.47551640107682	38.0	31.5	38.0	11.0	38.0
34-35	32.43683575957846	38.0	31.5	38.0	11.0	38.0
36-37	32.002695237244666	38.0	29.0	38.0	11.0	38.0
38-39	31.931831230304034	37.5	29.0	38.0	11.0	38.0
40-41	31.9244472371426	37.5	29.0	38.0	11.0	38.0
42-43	32.27576901976295	38.0	31.5	38.0	11.0	38.0
44-45	32.38181464422868	38.0	31.0	38.0	11.0	38.0
46-47	32.27936692226234	38.0	31.0	38.0	11.0	38.0
48-49	32.017077278352616	37.5	29.0	38.0	11.0	38.0
50-51	31.458174383444543	37.0	28.0	38.0	11.0	38.0
52-53	31.992408680896666	37.0	29.0	38.0	11.0	38.0
54-55	32.08071039436584	37.5	30.0	38.0	11.0	38.0
56-57	32.121971446433356	38.0	31.0	38.0	11.0	38.0
58-59	32.31827721711173	38.0	31.0	38.0	11.0	38.0
60-61	32.253438421005626	38.0	31.0	38.0	11.0	38.0
62-63	32.21141823702777	38.0	31.0	38.0	11.0	38.0
64-65	32.24349634468416	38.0	31.0	38.0	11.0	38.0
66-67	32.16583204684929	38.0	31.0	38.0	11.0	38.0
68-69	31.6241914288266	37.0	27.0	38.0	11.0	38.0
70-71	31.724811492874366	37.0	27.5	38.0	11.0	38.0
72-73	31.82697852741168	37.0	28.5	38.0	11.0	38.0
74-75	31.835616045114126	37.0	29.0	38.0	11.0	38.0
76	30.975854501843607	37.0	25.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	23.0
13	413.0
14	1739.0
15	3368.0
16	4598.0
17	5100.0
18	5214.0
19	4465.0
20	3420.0
21	2556.0
22	1848.0
23	1502.0
24	1275.0
25	1308.0
26	1410.0
27	1561.0
28	1785.0
29	2245.0
30	2814.0
31	3468.0
32	4500.0
33	6095.0
34	8445.0
35	12896.0
36	23656.0
37	51053.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.41627221577208	23.98665458860154	9.350081016598834	29.246992179027547
2	18.581507801834675	27.243904617308207	29.35926715063984	24.815320430217277
3	16.7972288495643	22.484976843287104	33.708008522691024	27.009785784457573
4	12.641779047959275	22.59151048112377	30.580257466923538	34.186453003993414
5	12.955638627693641	32.70327511195601	35.901835950956254	18.439250309394097
6	24.705116836887665	41.922848740407126	16.09369916494957	17.278335257755632
7	24.97097288676236	28.626475279106856	19.051355661881978	27.351196172248805
8	21.928128967228698	36.19260875389945	20.238209157081524	21.641053121790332
9	22.779092689586538	18.897728215172027	19.08656514555117	39.236613949690266
10-11	21.91005680857647	26.949784535691162	24.605992172473517	26.534166483258854
12-13	21.98484287883234	23.43771928705393	25.84429502800495	28.73314280610878
14-15	24.266143565698613	25.520628997017685	22.414876481189097	27.798350956094602
16-17	24.973845035022137	26.34666173337246	22.792457163270775	25.88703606833463
18-19	21.43381156822343	26.566277741982812	25.098719674400506	26.90119101539325
20-21	23.670562267954427	25.784011023360847	24.352186172316564	26.19324053636816
22-23	25.321518793539006	25.203182015361453	23.228456964964657	26.246842226134888
24-25	21.32754936046697	28.917099933016488	23.108034831424835	26.647315875091703
26-27	22.99858061017814	28.792880723410363	24.24094540931056	23.967593257100937
28-29	22.006909157025426	27.767410852416454	25.853197957237228	24.372482033320892
30-31	22.68464767348397	27.04519067607395	25.51257352096863	24.757588129473458
32-33	21.009521075546626	28.63977800105258	25.034846817536643	25.315854105864155
34-35	20.77318107875347	29.3116646996906	24.89170999330165	25.02344422825428
36-37	23.72945158501533	25.947866920600895	24.786022133111302	25.53665936127248
38-39	23.33515153255055	25.57121758740913	26.506154763841437	24.58747611619888
40-41	21.592268312520932	26.891536290129658	26.064462625392725	25.451732771956685
42-43	19.726966811795286	26.236065260035407	26.88228633398721	27.154681594182094
44-45	19.334496518419048	25.056537812552833	28.688354645988767	26.92061102303936
46-47	20.578037632093498	25.894631414090185	27.336057338066848	26.191273615749466
48-49	20.369041353683237	26.392357622442308	27.097268073297933	26.14133295057653
50-51	22.85710640388118	26.18966358651794	25.601490209337413	25.351739800263466
52-53	18.265421347776993	27.56161202447256	26.806254665161056	27.366711962589395
54-55	18.91365995412976	26.27427087686168	26.618776176820536	28.193292992188024
56-57	19.691208370549955	27.18514737782315	25.767194079367105	27.356450172259795
58-59	19.450496518152498	27.054417623922188	25.484635530475337	28.010450327449977
60-61	18.344494035750404	25.98715588720406	26.385942300391445	29.282407776654086
62-63	19.338713793543448	26.370422355493172	25.811216026540766	28.47964782442261
64-65	20.009379396592152	25.385943028141376	25.182722768644737	29.421954806621724
66-67	18.99232472230552	28.598863063603364	23.415339071182935	28.993473142908183
68-69	19.859655837838698	29.657273176722004	22.635599572588234	27.84747141285106
70-71	20.33555013316747	30.13827089612937	22.300687367430587	27.225491603272573
72-73	21.167918136638832	30.05601311651111	21.945275567946208	26.83079317890385
74-75	22.22062731062817	29.810492604394938	21.66304621734817	26.30583386762872
76	21.28849932378984	29.984817168082877	22.37043558141315	26.356247926714126
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	1.5
15	2.5
16	12.5
17	23.0
18	20.5
19	22.5
20	33.5
21	40.0
22	62.0
23	123.0
24	235.5
25	309.0
26	360.5
27	496.5
28	676.5
29	772.0
30	935.5
31	1420.5
32	1902.5
33	2063.0
34	2389.5
35	3120.5
36	3932.5
37	4340.0
38	4844.5
39	5997.5
40	7045.0
41	8041.0
42	8638.0
43	8845.0
44	9487.0
45	10009.0
46	10096.0
47	10147.0
48	9928.5
49	9190.5
50	8722.0
51	8437.5
52	7633.5
53	6600.0
54	6086.0
55	5685.5
56	4856.0
57	3941.0
58	3455.0
59	3053.5
60	2520.5
61	2132.0
62	1875.0
63	1824.0
64	1584.0
65	1297.0
66	1112.5
67	1026.0
68	972.0
69	908.5
70	792.5
71	686.0
72	689.5
73	644.5
74	555.5
75	515.0
76	462.0
77	377.0
78	318.0
79	291.0
80	238.0
81	178.5
82	178.5
83	185.0
84	151.0
85	102.0
86	69.0
87	51.0
88	42.5
89	31.5
90	22.5
91	16.0
92	16.0
93	15.5
94	11.0
95	5.5
96	4.0
97	5.0
98	5.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	6.379259750698529E-4
7	0.005103407800558823
8	0.0031896298753492642
9	0.005741333775628676
10-11	0.002232740912744485
12-13	0.0
14-15	3.1896298753492645E-4
16-17	0.0
18-19	6.379259750698529E-4
20-21	0.0
22-23	0.0012758519501397058
24-25	0.0019137779252095587
26-27	3.1896298753492645E-4
28-29	0.006060296763163602
30-31	0.0
32-33	3.1896298753492645E-4
34-35	0.0019137779252095587
36-37	0.015629186389211396
38-39	0.006060296763163602
40-41	3.1896298753492645E-4
42-43	3.1896298753492645E-4
44-45	0.002870666887814338
46-47	0.002232740912744485
48-49	3.1896298753492645E-4
50-51	9.568889626047793E-4
52-53	0.007017185725768382
54-55	0.007336148713303308
56-57	0.011482667551257352
58-59	0.010525778588652572
60-61	0.020732594189770217
62-63	0.011482667551257352
64-65	0.020094668214700368
66-67	0.01339644547646691
68-69	3.1896298753492645E-4
70-71	3.1896298753492645E-4
72-73	0.005741333775628676
74-75	0.006060296763163602
76	0.0012758519501397058
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	156758.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.42095110539353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.65099908081355	51.95614068386227
2	7.18499991811193	9.257290568338902
3	4.566999947949504	8.826314410355693
4	2.8710010894362377	7.398104832244136
5	1.770999997112882	5.704475211083052
6	1.0289999885234693	3.977349476887255
7	0.5169999941108566	2.3313941939472964
8	0.35699999593129433	1.839862342601248
9	0.2509999971393321	1.4552692688850146
>10	0.8009999908709333	7.253799011795131
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0031896298753492647	0.0
2	0.0	0.0	0.0	0.005741333775628676	0.0
3	0.0	0.0	0.0	0.005741333775628676	0.0
4	0.0	0.0	0.0	0.007655111700838235	0.0
5	0.0	0.0	0.0	0.00893096365097794	0.0
6	0.0	0.0	0.0	0.00893096365097794	0.0
7	0.0	0.0	0.0	0.010844741576187499	0.0
8	0.0	0.0	0.0	0.010844741576187499	0.0
9	0.0	0.0	0.0	0.010844741576187499	0.0
10	0.0	0.0	0.0	0.010844741576187499	0.0
11	0.0	0.0	0.0	0.012758519501397059	0.0
12	0.0	0.0	0.0	0.013396445476466911	0.0
13	0.0	0.0	0.0	0.014672297426606617	0.0
14	0.0	0.0	0.0	0.016586075351816176	0.0
15	0.0	0.0	0.0	0.017224001326886028	0.0
16	0.0	0.0	0.0	0.019137779252095588	0.0
17	0.0	0.0	0.0	0.019137779252095588	0.0
18	0.0	0.0	0.0	0.023603261077584557	0.0
19	0.0	0.0	0.0	0.023603261077584557	0.0
20	0.0	0.0	0.0	0.02424118705265441	0.0
21	0.0	0.0	0.0	0.02424118705265441	0.0
22	0.0	0.0	0.0	0.04401689227981985	0.0
23	0.0	0.0	0.0	0.044654818254889705	0.0
24	0.0	0.0	0.0	0.051034078005588235	0.0
25	0.0	0.0	0.0	0.05230992995572794	0.0
26	0.0	0.0	0.0	0.05230992995572794	0.0
27	0.0	0.0	0.0	0.07655111700838235	0.0
28	0.0	0.0	0.0	0.0988785261358272	0.0
29	0.0	0.0	0.0	0.13970578854029778	0.0
30	0.0	0.0	0.0	0.19010194057081617	0.0
31	0.0	0.0	0.0	0.23220505492542645	0.0
32	0.0	0.0	0.0	0.2711185394046875	0.0
33	0.0	0.0	0.0	0.3291698031360441	0.0
34	0.0	0.0	0.0	0.3993416603937279	0.0
35	0.0	0.0	0.0	0.49439263067913597	0.0
36	0.0	0.0	0.0	0.6921496829507904	0.0
37	0.0	0.0	0.0	1.0449227471644191	0.0
38	0.0	0.0	0.0	1.4646780387603822	0.0
39	0.0	0.0	0.0	1.999260005868919	0.0
40	0.0	0.0	0.0	2.633358425088353	0.0
41	0.0	0.0	0.0	3.386749001645849	0.0
42	0.0	0.0	0.0	4.144605060028835	0.0
43	0.0	0.0	0.0	4.934357417165312	0.0
44	0.0	0.0	0.0	5.701782365174346	0.0
45	0.0	0.0	0.0	6.457086719657051	0.0
46	0.0	0.0	0.0	7.218770333890455	0.0
47	0.0	0.0	0.0	8.039143137830287	0.0
48	0.0	0.0	0.0	8.833360976792253	0.0
49	0.0	0.0	0.0	9.573355107873283	0.0
50	0.0	0.0	0.0	10.249556641447326	0.0
51	0.0	0.0	0.0	10.960206177675143	0.0
52	0.0	0.0	0.0	11.75569986858725	0.0
53	0.0	0.0	0.0	12.509090445144745	0.0
54	0.0	0.0	0.0	13.241429464524936	0.0
55	0.0	0.0	0.0	14.034371451536764	0.0
56	0.0	0.0	0.0	14.607866903124561	0.0
57	0.0	0.0	0.0	15.251534211970043	0.0
58	0.0	0.0	0.0	15.849908776585565	0.0
59	0.0	0.0	0.0	16.47380038020388	0.0
60	0.0	0.0	0.0	17.062606055193356	0.0
61	0.0	0.0	0.0	17.713290549764604	0.0
62	0.0	0.0	0.0	18.287423927327474	0.0
63	0.0	0.0	0.0	18.927901606297606	0.0
64	6.379259750698529E-4	0.0	0.0	19.4739662409574	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGTGC	15	0.002221312	70.0	28
AGTCCAT	35	2.9592957E-7	60.000004	21
TAGTCCA	30	8.346518E-6	58.333332	20
TGGTCGC	55	3.45608E-11	57.27273	49
TCGCCGT	25	2.3846503E-4	55.999996	52
CTTCCGA	45	2.683555E-8	54.444447	18
GATCGCT	20	0.0069408985	52.5	43
CGAATTT	20	0.0069408985	52.5	18
GGCGGTC	20	0.0069408985	52.5	47
GGCATGC	20	0.0069408985	52.5	25
ATTGCGG	20	0.0069408985	52.5	3
TCGAATT	20	0.0069408985	52.5	17
GCCGTAT	20	0.0069408985	52.5	54
AGGTCCA	20	0.0069408985	52.5	11
ATCGTCA	35	2.0791555E-5	50.000004	7
TTACGCT	35	2.0791555E-5	50.000004	13
GATCTCG	85	0.0	49.411766	41
TTTACGG	30	5.8637926E-4	46.666664	36
TACGGAC	30	5.8637926E-4	46.666664	38
GTAGATC	100	0.0	45.499996	38
>>END_MODULE