FastQCFastQC Report
Wed 25 May 2016
SRR1779806_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779806_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156758
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2346414.968295079039029No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG31562.0132943773204555No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC19711.25735209686268No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT6100.3891348447926103Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC4910.3132216537592978No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT2580.16458490156802202No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT2370.15118845609155515No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC2090.13332652878959925Illumina PCR Primer Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAA150.00222167369.9971350
GCTGATA150.00222167369.9971317
CGCAAGC150.00222167369.9971370
TCGCGGA150.00222167369.9971318
AACTCGT553.45608E-1157.2703837
AATCCAT252.3851328E-455.99770768
ACGCCAA252.3851328E-455.99770761
CAACCGA252.3851328E-455.99770739
CCGTATG252.3851328E-455.9977072
TACGCCA252.3851328E-455.99770760
TACCTCG252.3851328E-455.99770736
GGAATTC200.006933267752.514656
TAACGAC200.006933267752.514654
TATGATC200.006933267752.514652
AACGTTA200.00694202452.4978570
GCAACCC200.00694202452.4978527
GACAACG200.00694202452.4978513
ACATTCG200.00694202452.4978510
CCGTCAC200.00694202452.4978513
GCAATCT200.00694202452.4978540