##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1779806_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156758
Sequences flagged as poor quality	0
Sequence length	76
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.62408936067059	34.0	34.0	34.0	34.0	34.0
2	33.687269549241506	34.0	34.0	34.0	34.0	34.0
3	33.79458145676776	34.0	34.0	34.0	34.0	34.0
4	33.62151213973131	34.0	34.0	34.0	34.0	34.0
5	33.65335102514704	34.0	34.0	34.0	34.0	34.0
6	37.364185559907625	38.0	38.0	38.0	37.0	38.0
7	37.407366769160106	38.0	38.0	38.0	37.0	38.0
8	37.3282958445502	38.0	38.0	38.0	37.0	38.0
9	37.2492887125378	38.0	38.0	38.0	36.0	38.0
10-11	37.199744191684	38.0	38.0	38.0	36.5	38.0
12-13	37.34037816251802	38.0	38.0	38.0	36.5	38.0
14-15	37.35807741869633	38.0	38.0	38.0	37.0	38.0
16-17	37.29090381352148	38.0	38.0	38.0	36.0	38.0
18-19	37.16141121984205	38.0	38.0	38.0	36.0	38.0
20-21	37.17877556488345	38.0	38.0	38.0	36.0	38.0
22-23	37.18710687811786	38.0	38.0	38.0	36.0	38.0
24-25	37.34329348422409	38.0	38.0	38.0	36.5	38.0
26-27	37.2509441304431	38.0	38.0	38.0	36.0	38.0
28-29	36.80950573495451	38.0	37.5	38.0	35.0	38.0
30-31	37.08041694841731	38.0	38.0	38.0	36.0	38.0
32-33	37.15808762551194	38.0	38.0	38.0	36.0	38.0
34-35	37.07629594661836	38.0	38.0	38.0	36.0	38.0
36-37	37.04561489684737	38.0	38.0	38.0	36.0	38.0
38-39	36.49694433457941	38.0	37.0	38.0	34.0	38.0
40-41	36.90372102221258	38.0	37.5	38.0	35.5	38.0
42-43	37.24976715701909	38.0	38.0	38.0	36.5	38.0
44-45	37.041953201750474	38.0	38.0	38.0	35.5	38.0
46-47	37.156521517243135	38.0	38.0	38.0	36.0	38.0
48-49	36.829163423876295	38.0	37.5	38.0	35.0	38.0
50-51	36.97376529427525	38.0	37.5	38.0	35.5	38.0
52-53	36.900729149389505	38.0	38.0	38.0	35.5	38.0
54-55	36.90965373378073	38.0	38.0	38.0	35.5	38.0
56-57	36.787060309521685	38.0	37.0	38.0	35.0	38.0
58-59	36.86122239375343	38.0	37.5	38.0	35.5	38.0
60-61	36.54420508044247	38.0	37.0	38.0	34.0	38.0
62-63	36.41413197412572	38.0	37.0	38.0	34.0	38.0
64-65	36.78710815396981	38.0	37.0	38.0	34.5	38.0
66-67	36.568200665994716	38.0	37.0	38.0	34.5	38.0
68-69	36.590154888426746	38.0	37.0	38.0	34.0	38.0
70-71	35.46198599114559	38.0	36.5	38.0	28.5	38.0
72-73	33.33089539289861	38.0	34.0	38.0	20.0	38.0
74-75	32.961998749665085	38.0	34.0	38.0	11.0	38.0
76	32.76729098355427	38.0	33.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	3.0
23	12.0
24	33.0
25	62.0
26	129.0
27	236.0
28	436.0
29	808.0
30	1247.0
31	1995.0
32	3163.0
33	5201.0
34	9083.0
35	19705.0
36	27621.0
37	87023.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.536171299103295	15.107381051153586	11.332929125315122	39.02351852442799
2	17.126398652700342	16.25818140063027	43.85358323020197	22.76183671646742
3	16.01513160412866	18.08775309713061	41.53280853289784	24.36430676584289
4	11.465443549930466	29.391801375368402	33.34758034677656	25.795174727924568
5	11.545184296814199	29.92255578662652	45.552380101812986	12.979879814746297
6	23.949016956072416	46.50161395271692	16.915245154952217	12.634123936258437
7	23.222419270467856	26.00887992957297	19.202209775577643	31.56649102438153
8	20.927799538141596	45.04522895163245	15.631738093111675	18.39523341711428
9	19.791653376542186	16.665178172724836	16.449559193151227	47.09360925758175
10-11	20.89143775756261	29.681419768050116	29.16501869123107	20.262123783156202
12-13	20.275201265645133	19.20284770155271	31.078477653453096	29.44347337934906
14-15	18.461577718521543	19.289605634162214	20.81743834445451	41.43137830286174
16-17	28.007502009466823	29.60263591012899	20.14410747776828	22.24575460263591
18-19	28.801719848428785	23.128325189145052	20.863050051672005	27.206904910754155
20-21	19.013064723969432	21.19413363273326	21.023488434402072	38.76931320889524
22-23	19.055486801311574	30.276923665777822	20.952040725194248	29.715548807716353
24-25	27.88743086433142	30.795434972600905	20.482019941693196	20.835114221374486
26-27	28.366973296418685	32.00857372510494	20.885058497811915	18.739394480664465
28-29	19.318312303040354	31.488026129447938	21.66619885428495	27.527462713226758
30-31	27.754883323339158	23.525434108626033	29.337577667487462	19.38210490054734
32-33	27.278352619961982	30.505939090827898	23.952844511922837	18.262863777287283
34-35	20.4346827594126	31.280062261575164	30.02940838745072	18.255846591561514
36-37	18.585335357685096	29.900866303474142	32.63342221768586	18.880376121154903
38-39	17.9853659781319	23.417305655851695	31.650697253090755	26.946631112925658
40-41	18.343242450146086	19.85257530716136	32.506474948646954	29.2977072940456
42-43	27.24422358029574	20.015565393791704	31.38436315849909	21.355847867413466
44-45	18.341328672220875	29.29324181222011	32.81842075045612	19.5470087651029
46-47	26.990966968193007	20.517932099159214	24.868906212123147	27.62219472052463
48-49	25.880018882608862	19.72467114915985	23.54393396190306	30.851376006328223
50-51	18.969339870928227	19.861613110068298	32.51134880961116	28.65769820939232
52-53	16.564252761346886	19.886483278776392	41.766368677297294	21.782895282579425
54-55	15.88475764064629	20.210494739227144	34.396550898815086	29.508196721311474
56-57	25.292242295430395	20.27234460815757	25.235756479114606	29.199656617297425
58-59	16.706604595361245	20.536497736141644	40.162346882448475	22.594550786048632
60-61	25.252699287847463	28.74677744595043	25.128902161982797	20.87162110421931
62-63	16.828372613893418	40.47200482405904	21.49513925003749	21.204483312010055
64-65	15.902841137475834	41.53050091556979	20.777053970765568	21.789603976188804
66-67	16.012661055130472	41.69564967677296	20.118250681233686	22.17343858686288
68-69	16.1439526367499	41.81446416495477	20.251553468669055	21.790029729626276
70-71	16.63226798434355	40.5019761963245	20.240780142975172	22.624975676356783
72-73	17.434567334073478	36.49075456529229	20.518293071985504	25.556385028648727
74-75	18.02997339526219	35.18811527443712	20.616757794805377	26.16515353549531
76	18.1774681315316	35.7067207696921	20.764588038637726	25.35122306013858
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.5
12	3.0
13	4.0
14	3.5
15	5.5
16	15.5
17	22.0
18	36.5
19	44.0
20	51.5
21	66.0
22	96.0
23	165.5
24	248.0
25	291.0
26	405.0
27	523.0
28	700.0
29	873.0
30	962.0
31	1218.5
32	1709.0
33	2032.0
34	2194.0
35	2686.0
36	3395.0
37	3774.0
38	8052.5
39	14256.5
40	15232.5
41	12860.5
42	11438.0
43	10352.5
44	8793.0
45	8228.0
46	8137.0
47	8032.0
48	7643.5
49	6966.0
50	6572.0
51	6339.5
52	5438.0
53	4557.5
54	4346.0
55	3973.0
56	3347.0
57	2707.5
58	2321.0
59	2109.5
60	1789.0
61	1588.5
62	1497.0
63	1355.0
64	1079.5
65	921.5
66	864.0
67	831.0
68	810.0
69	755.5
70	692.0
71	662.0
72	646.0
73	559.0
74	459.5
75	431.0
76	409.0
77	338.5
78	271.5
79	253.0
80	247.0
81	201.5
82	143.5
83	125.0
84	97.5
85	59.5
86	40.0
87	31.0
88	27.0
89	18.0
90	12.0
91	8.0
92	5.0
93	6.5
94	5.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04656859618009926
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.379259750698529E-4
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.014991260414141543
52-53	0.026154964977863966
54-55	0.0507151150180533
56-57	0.051990966968193005
58-59	0.03604281759144669
60-61	0.03189629875349265
62-63	0.0277497799155386
64-65	0.015948149376746323
66-67	0.036361780578981616
68-69	0.007655111700838235
70-71	0.011163704563722425
72-73	0.020413631202235293
74-75	0.012120593526327205
76	0.012758519501397057
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	156758.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.571364778831065
#Duplication Level	Percentage of deduplicated	Percentage of total
1	38.15155615696887	8.992842470559715
2	14.760487144790257	6.958496536061956
3	12.72259810554804	8.996670026410134
4	10.108254397834912	9.530614067543603
5	7.631935047361299	8.994756248484926
6	5.177266576454668	7.322114341851771
7	3.3098782138024356	5.461284272573011
8	2.213802435723951	4.174587580857117
9	1.3504736129905277	2.8649255540387095
>10	4.495263870094723	16.30793962668572
>50	0.046008119079837616	0.7138391661031653
>100	0.021650879566982407	1.053853710815397
>500	0.002706359945872801	0.3891348447926103
>1k	0.005412719891745602	3.2706464741831356
>5k	0.0	0.0
>10k+	0.002706359945872801	14.968295079039029
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT	23464	14.968295079039029	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG	3156	2.0132943773204555	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	1971	1.25735209686268	No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT	610	0.3891348447926103	Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC	491	0.3132216537592978	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTACCTCGTATGCCGT	258	0.16458490156802202	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTAACTCGTATGCCGT	237	0.15118845609155515	No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC	209	0.13332652878959925	Illumina PCR Primer Index 10 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00446548182548897	0.0
2	0.0	0.0	0.0	0.007017185725768382	0.0
3	0.0	0.0	0.0	0.007017185725768382	0.0
4	0.0	0.0	0.0	0.00893096365097794	0.0
5	0.0	0.0	0.0	0.010844741576187499	0.0
6	0.0	0.0	0.0	0.011482667551257352	0.0
7	0.0	0.0	0.0	0.013396445476466911	0.0
8	0.0	0.0	0.0	0.013396445476466911	0.0
9	0.0	0.0	0.0	0.013396445476466911	0.0
10	0.0	0.0	0.0	0.014034371451536763	0.0
11	0.0	0.0	0.0	0.017224001326886028	0.0
12	0.0	0.0	0.0	0.018499853277025732	0.0
13	0.0	0.0	0.0	0.01977570522716544	0.0
14	0.0	0.0	0.0	0.022327409127444853	0.0
15	0.0	0.0	0.0	0.022327409127444853	0.0
16	0.0	0.0	0.0	0.02424118705265441	0.0
17	0.0	0.0	0.0	0.02424118705265441	0.0
18	0.0	0.0	0.0	0.029344594853213234	0.0
19	0.0	0.0	0.0	0.029344594853213234	0.0
20	0.0	0.0	0.0	0.029344594853213234	0.0
21	0.0	0.0	0.0	0.029344594853213234	0.0
22	0.0	0.0	0.0	0.0338100766787022	0.0
23	0.0	0.0	0.0	0.03572385460391176	0.0
24	0.0	0.0	0.0	0.03572385460391176	0.0
25	0.0	0.0	0.0	0.038275558504191176	0.0
26	0.0	0.0	0.0	0.038913484479261025	0.0
27	0.0	0.0	0.0	0.0663443014072647	0.0
28	0.0	0.0	0.0	0.08930963650977941	0.0
29	0.0	0.0	0.0	0.13396445476466912	0.0
30	0.0	0.0	0.0	0.18627438472039703	0.0
31	0.0	0.0	0.0	0.23092920297528674	0.0
32	0.0	0.0	0.0	0.2685668355044081	0.0
33	0.0	0.0	0.0	0.32980772911111395	0.0
34	0.0	0.0	0.0	0.39997958636879777	0.0
35	0.0	0.0	0.0	0.5039615203051838	0.0
36	0.0	0.0	0.0	0.7151150180533051	0.0
37	0.0	0.0	0.0	1.0959568251700074	0.0
38	0.0	0.0	0.0	1.5342119700429961	0.0
39	0.0	0.0	0.0	2.0713456410518125	0.0
40	0.0	0.0	0.0	2.7111853940468746	0.0
41	0.0	0.0	0.0	3.483075823881397	0.0
42	0.0	0.0	0.0	4.2511386978655	0.0
43	0.0	0.0	0.0	5.062580538154353	0.0
44	0.0	0.0	0.0	5.849781191390551	0.0
45	0.0	0.0	0.0	6.622947473175213	0.0
46	0.0	0.0	0.0	7.4216307939626684	0.0
47	0.0	0.0	0.0	8.256037969354036	0.0
48	0.0	0.0	0.0	9.06110054989219	0.0
49	0.0	0.0	0.0	9.845111573253039	0.0
50	0.0	0.0	0.0	10.53534747827862	0.0
51	0.0	0.0	0.0	11.253652126207275	0.0
52	0.0	0.0	0.0	12.045956187244032	0.0
53	0.0	0.0	0.0	12.807001875502367	0.0
54	0.0	0.0	0.0	13.532961635131858	0.0
55	0.0	0.0	0.0	14.328455326043965	0.0
56	0.0	0.0	0.0	14.896209443856135	0.0
57	0.0	0.0	0.0	15.552635272203014	0.0
58	0.0	0.0	0.0	16.146544354993047	0.0
59	0.0	0.0	0.0	16.78574618201304	0.0
60	0.0	0.0	0.0	17.364982967376466	0.0
61	0.0	0.0	0.0	18.020770869748276	0.0
62	0.0	0.0	0.0	18.60383521096212	0.0
63	0.0	0.0	0.0	19.244312889932253	0.0
64	0.0	0.0	0.0	19.802498118118372	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGTAA	15	0.002221673	69.99713	50
GCTGATA	15	0.002221673	69.99713	17
CGCAAGC	15	0.002221673	69.99713	70
TCGCGGA	15	0.002221673	69.99713	18
AACTCGT	55	3.45608E-11	57.27038	37
AATCCAT	25	2.3851328E-4	55.997707	68
ACGCCAA	25	2.3851328E-4	55.997707	61
CAACCGA	25	2.3851328E-4	55.997707	39
CCGTATG	25	2.3851328E-4	55.997707	2
TACGCCA	25	2.3851328E-4	55.997707	60
TACCTCG	25	2.3851328E-4	55.997707	36
GGAATTC	20	0.0069332677	52.5146	56
TAACGAC	20	0.0069332677	52.5146	54
TATGATC	20	0.0069332677	52.5146	52
AACGTTA	20	0.006942024	52.49785	70
GCAACCC	20	0.006942024	52.49785	27
GACAACG	20	0.006942024	52.49785	13
ACATTCG	20	0.006942024	52.49785	10
CCGTCAC	20	0.006942024	52.49785	13
GCAATCT	20	0.006942024	52.49785	40
>>END_MODULE