FastQCFastQC Report
Wed 25 May 2016
SRR1779805_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779805_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189987
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTT207.912055E-569.9992114
CTTCCGA301.0216354E-769.9807818
GCCGTAT509.731593E-1056.0141254
CGTATCA452.6822818E-854.45816856
GACGAAT452.6877387E-854.4438323
ACGAATA452.6877387E-854.4438324
GAGCGAC200.006943458752.4994126
ATCTCGA305.8748934E-446.65385442
TCTCGGT1050.046.65385443
TAGATCT1600.045.9248939
TCGGTGG1150.045.6396445
GTGTAGA1950.044.87129236
CGCCGTA950.044.21002653
TACCGAC404.5805653E-543.74950819
AGATCTC1850.043.50156840
CGACGAG1050.043.33284422
GACGATC659.949872E-943.07643523
CGACGAT757.4942363E-1041.99952722
TCGCCGT1000.041.99952352
GATCTCG1500.041.98847241